570 research outputs found
Assembly of proteasome subunits into non-canonical complexes in vivo
Proteasomes exist in all domains of life. In general, they are comprised of a compartmentalized protease whose activity is modulated by one or more regulatory complexes with which it interacts. The quaternary structure of this compartmentalized protease, called the 20S proteasome, is absolutely conserved and consists of four heptameric rings stacked coaxially. The rings are made of structurally related α and β subunits. In eukaryotes, assembly factors chaperone the α and β subunits during 20S biogenesis. Here we demonstrate that proteasome subunits can assemble into structures other than the canonical 20S proteasome in vivo. Specifically, the yeast α4 subunit forms high molecular weight complexes whose abundance increases when proteasome function is compromised. Results from a disulfide crosslinking approach are consistent with these complexes being ring-shaped. Though several eukaryotic α subunits can form rings when expressed recombinantly in bacteria, this is the first evidence that such non-canonical complexes exist in vivo
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Using Helicopter Electromagnetic Surveys to Identify Potential Hazards at Mine Waste Impoundments
In July 2003, helicopter electromagnetic surveys were conducted at 14 coal waste impoundments in southern West Virginia. The purpose of the surveys was to detect conditions that could lead to impoundment failure either by structural failure of the embankment or by the flooding of adjacent or underlying mine works. Specifically, the surveys attempted to: 1) identify saturated zones within the mine waste, 2) delineate filtrate flow paths through the embankment or into adjacent strata and receiving streams, and 3) identify flooded mine workings underlying or adjacent to the waste impoundment. Data from the helicopter surveys were processed to generate conductivity/depth images. Conductivity/depth images were then spatially linked to georeferenced air photos or topographic maps for interpretation. Conductivity/depth images were found to provide a snapshot of the hydrologic conditions that exist within the impoundment. This information can be used to predict potential areas of failure within the embankment because of its ability to image the phreatic zone. Also, the electromagnetic survey can identify areas of unconsolidated slurry in the decant basin and beneath the embankment. Although shallow, flooded mineworks beneath the impoundment were identified by this survey, it cannot be assumed that electromagnetic surveys can detect all underlying mines. A preliminary evaluation of the data implies that helicopter electromagnetic surveys can provide a better understanding of the phreatic zone than the piezometer arrays that are typically used
Instrument manual for the retarding ion mass spectrometer on Dynamics Explorer-1
The retarding ion mass spectrometer (RIMS) for Dynamics Explorer-1 is an instrument designed to measure the details of the thermal plasma distribution. It combines the ion temperature determining capability of the retarding potential analyzer with the compositional capabilities of the mass spectrometer and adds multiple sensor heads to sample all directions relative to the spacecraft ram direction. This manual provides a functional description of the RIMS, the instrument calibration, and a description of the commands which can be stored in the instrument logic to control its operation
Molecular chaperones of the Hsp70 family assist in the assembly of 20S proteasomes
The eukaryotic 26S proteasome is a large protease comprised of two major sub assemblies, the 20S proteasome, or core particle (CP), and the 19S regulatory particle (RP). Assembly of the CP and RP is assisted by an expanding list of dedicated assembly factors. For the CP, this includes Ump1 and the heterodimeric Pba1–Pba2 and Pba3–Pba4 proteins. It is not known how many additional proteins that assist in proteasome biogenesis remain to be discovered. Here, we demonstrate that two members of the Hsp70 family in yeast, Ssa1 and Ssa2, play a direct role in CP assembly. Ssa1 and Ssa2 interact genetically and physically with proteasomal components. Specifically, they associate tightly with known CP assembly intermediates, but not with fully assembled CP, through an extensive purification protocol. And, in yeast lacking both Ssa1 and Ssa2, specific defects in CP assembly are observed
Pancharatnam-Berry phase in condensate of indirect excitons
We report on the observation of the Pancharatnam-Berry phase in a condensate
of indirect excitons (IXs) in a GaAs coupled quantum well structure. The
Pancharatnam-Berry phase leads to phase shifts of interference fringes in IX
interference patterns. Correlations are found between the phase shifts,
polarization pattern of IX emission, and onset of IX spontaneous coherence. The
Pancharatnam-Berry phase is acquired due to coherent spin precession in IX
condensate. The effect of the Pancharatnam-Berry phase on the IX phase pattern
is described in terms of an associated momentum.Comment: 6 pages, 5 figures + 2 pages supplemental material, 3 supplemental
figure
Space capsule Patent
Manned space capsule configuration for orbital flight and atmospheric reentr
The retarding ion mass spectrometer on dynamics Explorer-A
An instrument designed to measure the details of the thermal plasma distribution combines the ion temperature-determining capability of the retarding potential analyzer with the compositional capabilities of the mass spectrometer and adds multiple sensor heads to sample all directions relative to the spacecraft ram directions. The retarding ion mass spectrometer, its operational modes and calibration are described as well as the data reduction plan, and the anticipated results
Covering Partial Cubes with Zones
A partial cube is a graph having an isometric embedding in a hypercube.
Partial cubes are characterized by a natural equivalence relation on the edges,
whose classes are called zones. The number of zones determines the minimal
dimension of a hypercube in which the graph can be embedded. We consider the
problem of covering the vertices of a partial cube with the minimum number of
zones. The problem admits several special cases, among which are the problem of
covering the cells of a line arrangement with a minimum number of lines, and
the problem of finding a minimum-size fibre in a bipartite poset. For several
such special cases, we give upper and lower bounds on the minimum size of a
covering by zones. We also consider the computational complexity of those
problems, and establish some hardness results
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New Strategies for Finding Abandoned Wells at Proposed Geologic Storage Sites for CO2
Prior to the injection of CO2 into geological formations, either for enhanced oil recovery or for CO2 sequestration, it is necessary to locate wells that perforate the target formation and are within the radius of influence for planned injection wells. Locating and plugging wells is necessary because improperly plugged well bores provide the most rapid route for CO2 escape to the surface. This paper describes the implementation and evaluation of helicopter and ground-based well detection strategies at a 100+ year old oilfield in Wyoming where a CO2 flood is planned. This project was jointly funded by the U.S. Department of Energy’s National Energy Technology Laboratory and Fugro Airborne Surveys
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