Разработка Android-приложения с функциями заказа услуг в многофункциональном комплексе

This article represents advantages of mobile applications and describes some useful elements of Android app implementation such as Navigation Drawer and ViewPager

Structural Characterization of LRRK2 Inhibitors

Kinase inhibition is considered to be an important therapeutic target for LRRK2 mediated Parkinson's disease (PD). Many LRRK2 kinase inhibitors have been reported but have yet to be optimized in order to qualify as drug candidates for the treatment of the disease. In order to start a structure-function analysis of such inhibitors, we mutated the active site of Dictyostelium Roco4 kinase to resemble LRRK2. Here, we show saturation transfer difference (STD) NMR and the first cocrystal structures of two potent in vitro inhibitors, LRRK2-IN-1 and compound 19, with mutated Roco4. Our data demonstrate that this system can serve as an excellent tool for the structural characterization and optimization of LRRK2 inhibitors using X-ray crystallography and NMR spectroscopy

Photocatalytic degradation of diclofenac in wastewater using ironceramic composites

The photocatalytic degradation of diclofenac in model aqueous solution is investigated. The surface of composites were correlated with photocatalytic activity. Adsorption activity of the composites is related to the presence of surface active sites. The degradation under UV irradiation and photo-Fenton process using ironceramic composites is more effective than direct photolysis

Построение расчётной модели для анализа режимов Акмолинской энергосистемы в перспективе её развития до 2020 года.

Объектом исследования является энергосистема Акмолинской области Республики Казахстан. Цель работы – составить расчетную модель для решения режимных проблем энергосистем с нетрадиционными источниками электроэнергии. В процессе исследования проводилась апробация цифровых модели на примере перспективной схемы развития Макинской ВЭС. В результате исследования построено цифровое моделирование Буландинского энергорайона Акмолинской в ПК "RastrWin"; анализ статической устойчивости энергосистемы;The object of research is the energy system of Akmola Oblast of the Republic of Kazakhstan. The purpose of the work is to make a design model for solving the regime problems of power systems with non-traditional sources of electricity. In the course of the research the approbation of the digital model on the example of the prospective development scheme of MAKINSKOJ WEC was conducted

Проектирование теплообменного аппарата подогрева гидроксида натрия

Объектом исследования является кожухотрубчатый теплообменник. Цель работы – Разработка теплообменного аппарата подогрева гидроксида натрия. В ходе выполнения работы были произведены все необходимые расчеты для конструирования аппарата, определены его размеры и основные механические характеристики. Основные конструктивные, технологические и технико-эксплуатационные характеристики: Аппарат диаметром 600 мм, толщина стенки 8 мм, длина труб 3000 мм. Степень внедрения: подогрев гидроксида натрия. Область применения: химическая промышленность.The object of the study is a shell-and-tube heat exchanger. The purpose of the work is to develop a heat exchanger for heating sodium hydroxide. In the course of the work, all the necessary calculations were made for the design of the device, its dimensions and basic mechanical characteristics were determined. The main design, technological and technical-operational characteristics: The device with a diameter of 600 mm, wall thickness of 8 mm, pipe length of 3000 mm. Degree of introduction: heating of sodium hydroxide. Scope of application: chemical industry

Identification of the protein kinases Pyk3 and Phg2 as regulators of the STATc-mediated response to hyperosmolarity

Cellular adaptation to changes in environmental osmolarity is crucial for cell survival. In Dictyostelium, STATc is a key regulator of the transcriptional response to hyperosmotic stress. Its phosphorylation and consequent activation is controlled by two signaling branches, one cGMP- and the other Ca(2+)-dependent, of which many signaling components have yet to be identified. The STATc stress signalling pathway feeds back on itself by upregulating the expression of STATc and STATc-regulated genes. Based on microarray studies we chose two tyrosine-kinase like proteins, Pyk3 and Phg2, as possible modulators of STATc phosphorylation and generated single and double knock-out mutants to them. Transcriptional regulation of STATc and STATc dependent genes was disturbed in pyk3(-), phg2(-), and pyk3(-)/phg2(-) cells. The absence of Pyk3 and/or Phg2 resulted in diminished or completely abolished increased transcription of STATc dependent genes in response to sorbitol, 8-Br-cGMP and the Ca(2+) liberator BHQ. Also, phospho-STATc levels were significantly reduced in pyk3(-) and phg2(-) cells and even further decreased in pyk3(-)/phg2(-) cells. The reduced phosphorylation was mirrored by a significant delay in nuclear translocation of GFP-STATc. The protein tyrosine phosphatase 3 (PTP3), which dephosphorylates and inhibits STATc, is inhibited by stress-induced phosphorylation on S448 and S747. Use of phosphoserine specific antibodies showed that Phg2 but not Pyk3 is involved in the phosphorylation of PTP3 on S747. In pull-down assays Phg2 and PTP3 interact directly, suggesting that Phg2 phosphorylates PTP3 on S747 in vivo. Phosphorylation of S448 was unchanged in phg2(-) cells. We show that Phg2 and an, as yet unknown, S448 protein kinase are responsible for PTP3 phosphorylation and hence its inhibition, and that Pyk3 is involved in the regulation of STATc by either directly or indirectly activating it. Our results add further complexities to the regulation of STATc, which presumably ensure its optimal activation in response to different environmental cues

Subcoronary versus supracoronary aortic stenosis. an experimental evaluation

<p>Abstract</p> <p>Background</p> <p>Valvular aortic stenosis is the most common cause of left ventricular hypertrophy due to gradually increasing pressure work. As the stenosis develop the left ventricular hypertrophy may lead to congestive heart failure, increased risk of perioperative complications and also increased risk of sudden death. A functional porcine model imitating the pathophysiological nature of valvular aortic stenosis is very much sought after in order to study the geometrical and pathophysiological changes of the left ventricle, timing of surgery and also pharmacological therapy in this patient group.</p> <p>Earlier we developed a porcine model for aortic stenosis based on supracoronary aortic banding, this model may not completely imitate the pathophysiological changes that occurs when valvular aortic stenosis is present including the coronary blood flow. It would therefore be desirable to optimize this model according to the localization of the stenosis.</p> <p>Methods</p> <p>In 20 kg pigs subcoronary (n = 8), supracoronary aortic banding (n = 8) or sham operation (n = 4) was preformed via a left lateral thoracotomy. The primary endpoint was left ventricular wall thickness; secondary endpoints were heart/body weight ratio and the systolic/diastolic blood flow ratio in the left anterior descending coronary. Statistical evaluation by oneway anova and unpaired t-test.</p> <p>Results</p> <p>Sub- and supracoronary banding induce an equal degree of left ventricular hypertrophy compared with the control group. The coronary blood flow ratio was slightly but not significantly higher in the supracoronary group (ratio = 0.45) compared with the two other groups (subcoronary ratio = 0.36, control ratio = 0.34).</p> <p>Conclusions</p> <p>A human pathophysiologically compatible porcine model for valvular aortic stenosis was developed by performing subcoronary aortic banding. Sub- and supracoronary aortic banding induce an equal degree of left ventricular hypertrophy. This model may be valid for experimental investigations of aortic valve stenosis but studies of left ventricular hypertrophy can be studied equally well by graduated constriction of the ascending aorta.</p