24 research outputs found

    Application of the diode-array detector

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    THE USE OF BIOSENSORS IN DETERMINATION OF DIFFERENT SUBSTANCES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

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    A rapid, simple and economic method was developed, which combines the specificity of enzymes with the high sensitivity of HPLC. Therefore sample pretreatment is reduced to simple dilution or extraction steps. In this work oxidases for alcohol, glucose, oxalic acid, ascorbic acid and galactose were immobilized and used as biosensors. Food samples were fruit juices and vegetable products, soft drinks and wine. The detection limit e.g. for oxalic acid was in the picomol range
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