A pharmacological cocktail for arresting actin dynamics in living cells.

The actin cytoskeleton is regulated by factors that influence polymer assembly, disassembly, and network rearrangement. Drugs that inhibit these events have been used to test the role of actin dynamics in a wide range of cellular processes. Previous methods of arresting actin rearrangements take minutes to act and work well in some contexts, but can lead to significant actin reorganization in cells with rapid actin dynamics, such as neutrophils. In this paper, we report a pharmacological cocktail that not only arrests actin dynamics but also preserves the structure of the existing actin network in neutrophil-like HL-60 cells, human fibrosarcoma HT1080 cells, and mouse NIH 3T3 fibroblast cells. Our cocktail induces an arrest of actin dynamics that initiates within seconds and persists for longer than 10 min, during which time cells maintain their responsivity to external stimuli. With this cocktail, we demonstrate that actin dynamics, and not simply morphological polarity or actin accumulation at the leading edge, are required for the spatial persistence of Rac activation in HL-60 cells. Our drug combination preserves the structure of the existing cytoskeleton while blocking actin assembly, disassembly, and rearrangement, and should prove useful for investigating the role of actin dynamics in a wide range of cellular signaling contexts

Iron redox pathway revealed in ferritin via electron transfer analysis.

Ferritin protein is involved in biological tissues in the storage and management of iron - an essential micro-nutrient in the majority of living systems. While there are extensive studies on iron-loaded ferritin, its functionality in iron delivery is not completely clear. Here, for the first time, differential pulse voltammetry (DPV) has been successfully adapted to address the challenge of resolving a cascade of fast and co-occurring redox steps in enzymatic systems such as ferritin. Using DPV, comparative analysis of ferritins from two evolutionary-distant organisms has allowed us to propose a stepwise resolution for the complex mix of concurrent redox steps that is inherent to ferritins and to fine-tune the structure-function relationship of each redox step. Indeed, the cyclic conversion between Fe3+ and Fe2+ as well as the different oxidative steps of the various ferroxidase centers already known in ferritins were successfully discriminated, bringing new evidence that both the 3-fold and 4-fold channels can be functional in ferritin

Status of Spin Physics - Experimental Summary

The current status of spin physics experiments, based on talks presented at the Third Circum-Pan-Pacific Symposium on High Energy Spin Physics held in Beijing, 2001, is summarized in this article. Highlights of recent experimental results at SLAC, JLab, and DESY, as well as future plans at these facilities and at RHIC-spin are discussed.Comment: 18 pages, 7 figures, Invited talk presented at the Third Circum-Pan-Pacific Symposium on High Energy Spin Physics held in Beijing, October, 200

SN 1997bs in M66: Another Extragalactic Eta Carinae Analog?

We report on SN 1997bs in NGC 3627 (M66), the first supernova discovered by the Lick Observatory Supernova Search using the 0.75-m Katzman Automatic Imaging Telescope (KAIT). Based on its early-time optical spectrum, SN 1997bs was classified as Type IIn. However, from the BVRI light curves obtained by KAIT early in the supernova's evolution, and F555W and F814W light curves obtained from Hubble Space Telescope archival WFPC2 images at late times, we question the identification of SN 1997bs as a bona fide supernova. We believe that it is more likely a super-outburst of a very massive luminous blue variable star, analogous to Eta Carinae, and similar to SN 1961V in NGC 1058 (Filippenko et al. 1995 [AJ, 110, 2261]) and SN 1954J (``Variable 12'') in NGC 2403 (Humphreys & Davidson 1994 [PASP, 106, 1025]). The progenitor may have survived the outburst, since the SN is seen in early 1998 at m_F555W=23.4, about 0.5 mag fainter than the progenitor identified by Van Dyk et al. (1999, [AJ, 118, 2331]) in a pre-discovery image. Based on analysis of its environment in the Hubble Space Telescope images, the progenitor was not in an H II region or association of massive stars. The recent discovery of additional objects with properties similar to those of SN 1997bs suggests that the heterogeneous class of Type IIn supernovae consists in part of ``impostors.''Comment: 15 pages, 8 figures, to appear in the PASP (2000 Dec issue

Constructions for cyclic sieving phenomena

We show how to derive new instances of the cyclic sieving phenomenon from old ones via elementary representation theory. Examples are given involving objects such as words, parking functions, finite fields, and graphs.Comment: 18 pages, typos fixed, to appear in SIAM J. Discrete Mat

Formation and kinetics of transient metastable states in mixtures under coupled phase ordering and chemical demixing

We present theory and simulation of simultaneous chemical demixing and phase ordering in a polymer-liquid crystal mixture in conditions where isotropic- isotropic phase separation is metastable with respect to isotropic-nematic phase transition. It is found that mesophase formation proceeds by a transient metastable phase that surround the ordered phase, and whose lifetime is a function of the ratio of diffusional to orientational mobilities. It is shown that kinetic phase ordering in polymer-mesogen mixtures is analogous to kinetic crystallization in polymer solutions.Comment: 17 pages, 5 figures accepted for publication in EP

Strong laws of large numbers for sub-linear expectations

We investigate three kinds of strong laws of large numbers for capacities with a new notion of independently and identically distributed (IID) random variables for sub-linear expectations initiated by Peng. It turns out that these theorems are natural and fairly neat extensions of the classical Kolmogorov's strong law of large numbers to the case where probability measures are no longer additive. An important feature of these strong laws of large numbers is to provide a frequentist perspective on capacities.Comment: 10 page

Fully automated urban traffic system

The replacement of the driver with an automatic system which could perform the functions of guiding and routing a vehicle with a human's capability of responding to changing traffic demands was discussed. The problem was divided into four technological areas; guidance, routing, computing, and communications. It was determined that the latter three areas being developed independent of any need for fully automated urban traffic. A guidance system that would meet system requirements was not being developed but was technically feasible