26 research outputs found
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Impact of Intermediate Hyperglycemia and Diabetes on Immune Dysfunction in Tuberculosis
Supplementary Data:
Supplementary materials are available at Clinical Infectious Diseases online at https://academic.oup.com/cid/article/72/1/69/5857148#274319223 . Consisting of data provided by the authors to benefit the reader, the posted materials are not copyedited and are the sole responsibility of the authors, so questions or comments should be addressed to the corresponding author.Copyright © The Author(s) 2020. Background:
People with diabetes have an increased risk of developing active tuberculosis (TB) and are more likely to have poor TB-treatment outcomes, which may impact on control of TB as the prevalence of diabetes is increasing worldwide. Blood transcriptomes are altered in patients with active TB relative to healthy individuals. The effects of diabetes and intermediate hyperglycemia (IH) on this transcriptomic signature were investigated to enhance understanding of immunological susceptibility in diabetes-TB comorbidity.
Methods:
Whole blood samples were collected from active TB patients with diabetes (glycated hemoglobin [HbA1c] ≥6.5%) or IH (HbA1c = 5.7% to <6.5%), TB-only patients, and healthy controls in 4 countries: South Africa, Romania, Indonesia, and Peru. Differential blood gene expression was determined by RNA-seq (n = 249).
Results:
Diabetes increased the magnitude of gene expression change in the host transcriptome in TB, notably showing an increase in genes associated with innate inflammatory and decrease in adaptive immune responses. Strikingly, patients with IH and TB exhibited blood transcriptomes much more similar to patients with diabetes-TB than to patients with only TB. Both diabetes-TB and IH-TB patients had a decreased type I interferon response relative to TB-only patients.
Conclusions:
Comorbidity in individuals with both TB and diabetes is associated with altered transcriptomes, with an expected enhanced inflammation in the presence of both conditions, but also reduced type I interferon responses in comorbid patients, suggesting an unexpected uncoupling of the TB transcriptome phenotype. These immunological dysfunctions are also present in individuals with IH, showing that altered immunity to TB may also be present in this group. The TB disease outcomes in individuals with IH diagnosed with TB should be investigated further.European Union’s Seventh Framework Programme (FP7 2007-2013 - Health) under grant agreement No 305279
Polymorphisms in autophagy genes and active pulmonary tuberculosis susceptibility in Romania
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Ontologies for supporting engineering design optimization
This paper presents an optimization ontology and its implementation into a prototype computational knowledge-based tool dubbed ONTOP (ontology for optimization). Salient feature of ONTOP include a knowledge base that incorporates both standardized optimization terminology, formal method definitions, and often unrecorded optimization details, such as any idealizations and assumptions that may be made when creating an optimization model, as well as the model developer’s rationale and justification behind these idealizations and assumptions. ONTOP was developed using Protégé, a Java-based, free open-source ontology development environment created by Stanford University. Two engineering design optimization case studies are presented. The first case study consists of the optimization of a structural beam element and demonstrates ONTOP ’s ability to address the variations in an optimal solution that may arise when different techniques and approaches are used. A second case study, a more complex design problem that deals with the optimization of an impeller of a pediatric left ventricular heart assist device, demonstrates the wealth of knowledge ONTOP is able to capture. Together, these test beds help illustrate the potential value of an ontology in representing application-specific knowledge while facilitating both the sharing and exchanging of this knowledge in engineering design optimization