NHS reforms and the working lives of midwives and physiotherapists

From 2000 the NHS was subjected to a series of far reaching reforms, the purposes of which were to increase the role of the primary care sector in commissioning and providing services, promote healthier life styles, reduce health inequality, and improve service standards. These were seen as requiring a greater leadership role from health professionals, closer and more cooperative working between health professionals, and between health professionals, social services, and community and other service providers. The project surveyed a random sample of midwives and physiotherapists to investigate their perceptions of the effectiveness of the reforms, and their effects on working lives. The predominant perception was that NHS reforms had negatively affected the funding of their services; and had done little to improve service quality, delivery or organisation. Although the potential existed for the reforms to improve services, the necessary resources and required staffing were not made available and the objectives of the reforms were only partially secured by intensifying of work. The downside of this was a deterioration of the sociopsychological wellbeing of midwives and physiotherapists, especially the former, exacerbating the shortage of skilled and experienced. Shortage of staff and the associated increased work burdens were demoralising and demotivating; morale and job satisfaction declined, and job insecurity and labour turnover increased

What is energy know-how and how can it be shared and acquired by householders?

Our aim in this short paper is to contribute to conceptual, practical and policy discussions about the role of householder knowledge in the context of policy ambitions to reduce domestic energy consumption. More specifically, we are interested in the characteristics of this knowledge, the ways in which householders acquire such knowledge, and the kinds of activities and policies that might support this. Within this context, literacy approaches emphasise factual knowledge, cognitive reasoning, and ideal attitudes and behaviours; within this mainstream approach, education and communications are key policy recommendations. In contrast, know-how approaches are critical of literacy approaches and emphasise practical skills, experience and guidance. Key policy recommendations focus on tailored guidance delivered through activities such as demonstration homes and home audits. Smart Communities was a community action and action research project on energy demand reduction. The activities in the project drew on both literacy and know-how approaches, and the research methods focussed on in-depth interviews, a survey and informal interactions with project participants and partners. The project strongly supports the ideas that are expressed in the know-how literature, but also highlights the practical challenge of scaling-up activities such as home visits. Meanwhile, approaches that drew on literacy approaches produced less change, but were easier to implement at scale. In our discussion, we raise the need for know-how approaches to be more adequately supported in policy, and the need to investigate and experiment with novel approaches that would allow these activities to be scaled-up. In support of these objectives, we present a concise expression of the concept of energy know-how. In addition, we suggest that the know-how literature is perhaps overly critical of the literacy approach, and we discuss some ways in which literacy approaches can be more effective

The diet of Cape clawless otters at two sites along the Bloukrans River, Eastern Cape Province, South Africa

The diet of Cape clawless otters was assessed at two sites along the Bloukrans River using faecal analysis. Spraints (n = 78) were collected during 2000 and 2001 and analysed using the relative frequency of occurrence and the reconstituted wet weight methods. Both methods found crab (Potamonautes perlatus) to be the most important component (>50%) of the diet. Frog, Xenopus and Rana spp., (11–42%) was the second most important component while fish (Micropteris salmoides) was relatively unimportant (<14%). Although the diet of Cape clawless otters in the Eastern Cape Province was similar to that reported elsewhere, the fact that they were preying on an alien invasive fish and not the indigenous endemic Sandelia bainsii is significant. The validity of faecal analysis methods is also discussed

Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. coli cytoplasm

<p>Abstract</p> <p>Background</p> <p>Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events. However they suffer in some cases from batch to batch variation, partial loss of binding and susceptibility to photo-bleaching. In theory, these issues can all be addressed by using recombinant antibody fused directly to genetically encoded fluorescent reporters. However, single-chain fragment variable domains linked by long flexible linkers are themselves prone to disassociation and aggregation, and in some cases with isoelectric points incompatible with use in physiologically relevant milieu. Here we describe a general approach that permits fully functional intracellular production of a range of coloured fluorescent recombinant antibodies with optimally orientated V_H/V_Linterfaces and isoelectric points compatible for use in physiological solutions at pH 7.4 with a binding site to fluorophore stoichiometry of 1:1.</p> <p>Results</p> <p>Here we report the design, assembly, intracellular bacterial production and purification of a panel of novel antibody fluorescent protein fusion constructs. The insertion of monomeric fluorescent protein derived from either <it>Discosoma </it>or <it>Aequorea </it>in-between the variable regions of anti-p185^HER2-ECDantibody 4D5-8 resulted in optimal V_H/V_Linterface interactions to create soluble coloured antibodies each with a single binding site, with isoelectric points of 6.5- 6. The fluorescent antibodies used in cell staining studies with SK-BR-3 cells retained the fluorophore properties and antibody specificity functions, whereas the conventional 4D5-8 single chain antibody with a (Gly₄Ser)₃linker precipitated at physiological pH 7.4.</p> <p>Conclusions</p> <p>This modular monomeric recombinant fluorescent antibody platform may be used to create a range of recombinant coloured antibody molecules for quantitative <it>in situ, in vivo </it>and <it>ex vivo </it>imaging, cell sorting and cell trafficking studies. Assembling the single chain antibody with monomeric fluorescent protein linker facilitates optimal variable domain pairing and alters the isoelectric point of the recombinant 4D5-8 protein conferring solubility at physiological pH 7.4. The efficient intracellular expression of these functional molecules opens up the possibility of developing an alternative approach for tagging intracellular targets with fluorescent proteins for a range of molecular cell biology imaging studies.</p

Correction: Messer, T.L.; Burchell, M.R.; Birgand, F. Comparison of Four Nitrate Removal Kinetic Models in Two Distinct Wetland Restoration Mesocosm Systems. Water 2017, 9, 517

The authors wish to make the following corrections to this paper [1]: (1): The author name “François Bírgand” should be François Birgand. (2): Reference 38 should be the following: Birgand, F.; Aveni-Deforge, K.; Smith, B.; Maxwell, B.M.; Horstman, M.; Gerling, A.B.; Carey, C.C. First report of a novel multiplexer pumping system coupled to a water quality probe to collect high temporal frequency in situ water chemistry measurements at multiple sites. Limnol. Oceanogr. Methods 2016, 14, 767–783, doi:10.1002/lom3.10122. (3): Reference 49 should be the following: Christensen, P.B.; Nielsen, L.P.; Sørensen, J.; Revsbech, N.P. Denitrification in Nitrate-Rich Streams: Diurnal and Seasonal Variation Related to Benthic Oxygen Metabolism. Limnol. Oceanogr. 1990, 35, 640–651

‘Younger People Have Like More of an Imagination, No Offence’: Participant Perspectives on Public Engagement

© 2012, Copyright Taylor & Francis Group, LLC. A wide range of work has reported on the outcomes of public engagement activities and the views expressed by public participants towards specific areas of science and technology. Such work has rarely gone on to explore with public participants their attitudes to the engagement experienced itself, often focusing instead on more practical or quantifiable aspects. This article draws on public participants’ reactions to 11 ‘engagement’ events, occurring across the UK in 2007–2008. Reporting on 33 semi-structured interviews, we focus on their views of participation and engagement in terms of motivations, expectations and expertise. The results suggest that participants have considerable expectations in terms of information and interaction, operate with critical but respectful notions of other ‘publics’ and expertise, and may develop habitual tendencies regarding engagement