Cytokine gene polymorphisms in preterm infants with necrotising enterocolitis: genetic association study

BACKGROUND The inflammatory cytokine cascade is implicated in the pathogenesis of necrotising enterocolitis (NEC). Genetic association studies of cytokine polymorphisms may help to detect molecular mechanisms that are causally related to the disease process. AIM To examine associations between the common genetic variants in candidate inflammatory cytokine genes and NEC in preterm infants. METHODS Multi-centre case-control and genetic association study. DNA samples were collected from 50 preterm infants with NEC and 50 controls matched for gestational age and ethnic group recruited to a multi-centre case-control study. Ten candidate single-nucleotide polymorphisms in cytokines previously associated with infectious or inflammatory diseases were genotyped. The findings were included in random-effects meta-analyses with data from previous genetic association studies. RESULTS All allele distributions were in Hardy-Weinberg equilibrium. None of the studied cytokine polymorphisms was significantly associated with NEC. Four previous genetic association studies of cytokine polymorphisms and NEC in preterm infants were found. Meta-analyses were possible for several single-nucleotide polymorphisms. These increased the precision of the estimates of effect size but did not reveal any significant associations. CONCLUSIONS The available data are not consistent with more than modest associations between these candidate cytokine variant alleles and NEC in preterm infants. Data from future association studies of these polymorphisms may be added to the meta-analyses to obtain more precise estimates of effects sizes.The study was funded by Tenovus (Scotland)

Theoretical He I Emissivities in the Case B Approximation

We calculate the He I case B recombination cascade spectrum using improved radiative and collisional data. We present new emissivities over a range of electron temperatures and densities. The differences between our results and the current standard are large enough to have a significant effect not only on the interpretation of observed spectra of a wide variety of objects but also on determinations of the primordial helium abundance.Comment: Accepted to ApJ

Maternal and perinatal outcomes in women planning vaginal birth after caesarean (VBAC) at home in England: secondary analysis of the Birthplace national prospective cohort study

OBJECTIVE: To compare vaginal birth rates in women planning vaginal birth after caesarean (VBAC) at home versus in an obstetric unit (OU) and explore transfer rates in women planning home VBAC. DESIGN: Prospective cohort study. SETTING: OUs and planned home births in England. POPULATION: 1436 women planning VBAC in the Birthplace cohort, including 209 planning home VBAC. METHODS: We used Poisson regression to calculate relative risks adjusted for maternal characteristics. MAIN OUTCOME MEASURES: MAIN OUTCOMES: (i) vaginal birth and (ii) transfer from planned home birth to OU during labour or immediately after birth. SECONDARY OUTCOMES: (i) composite of maternal blood transfusion or admission to higher level care, (ii) stillbirth or Apgar score <7 at 5 minutes, (iii) neonatal unit admission. RESULTS: Planned VBAC at home was associated with a statistically significant increase in the chances of having a vaginal birth compared with planned VBAC in an OU (adjusted relative risk 1.15, 95% confidence interval 1.06-1.24). The risk of an adverse maternal outcome was around 2-3% in both settings, with a similar risk of an adverse neonatal outcome. Transfer rates were high (37%) and varied markedly by parity (para 1, 56.7% versus para 2+, 24.6%). CONCLUSION: Women in the cohort who planned VBAC at home had an increased chance of a vaginal birth compared with those planning VBAC in an OU, but transfer rates were high, particularly for women with only one previous birth, and the risk of an adverse maternal or perinatal outcome was around 2-3%. No change in guidance can be recommended. TWEETABLE ABSTRACT: Higher vaginal birth rates in planned VBAC at home versus in OU but 2-3% adverse outcomes and high transfer rate

Physical Conditions of Accreting Gas in T Tauri Star Systems

We present results from a low resolution (R~300) near-infrared spectroscopic variability survey of actively accreting T Tauri stars (TTS) in the Taurus-Auriga star forming region. Paschen and Brackett series H I recombination lines were detected in 73 spectra of 15 classical T Tauri systems. The values of the Pan/PaB, Brn/BrG, and BrG/Pan H I line ratios for all observations exhibit a scatter of < 20% about the weighted mean, not only from source to source, but also for epoch-to-epoch variations in the same source. A representative or `global' value was determined for each ratio in both the Paschen and Brackett series as well as the BrG/Pan line ratios. A comparison of observed line ratio values was made to those predicted by the temperature and electron density dependent models of Case B hydrogen recombination line theory. The measured line ratios are statistically well-fit by a tightly constrained range of temperatures (T < 2000 K) and electron densities 1e9 < n_e < 1e10 cm^-3. A comparison of the observed line ratio values to the values predicted by the optically thick and thin local thermodynamic equilibrium cases rules out these conditions for the emitting H I gas. Therefore, the emission is consistent with having an origin in a non-LTE recombining gas. While the range of electron densities is consistent with the gas densities predicted by existing magnetospheric accretion models, the temperature range constrained by the Case B comparison is considerably lower than that expected for accreting gas. The cooler gas temperatures will require a non-thermal excitation process (e.g., coronal/accretion-related X-rays and UV photons) to power the observed line emission.Comment: 12 pages, emulateapj format, Accepted for publication in Ap

The young age of the extremely metal-deficient blue compact dwarf galaxy SBS 1415+437

We use Multiple Mirror Telescope (MMT) spectrophotometry and Hubble Space Telescope (HST) Faint Object Spectrograph (FOS) spectra and Wide Field and Planetary Camera 2 (WFPC2) V and I images to study the properties and evolutionary status of the nearby (D = 11.4 Mpc) extremely metal-deficient blue compact dwarf (BCD) galaxy SBS 1415+437=CG 389. The oxygen abundance in the galaxy is 12+log(O/H)=7.60+/-0.01 or Zsun/21. The helium mass fraction in SBS 1415+437 is Y=0.246+/-0.004 which agrees with the primordial helium abundance determined by Izotov & Thuan using a much larger sample of BCDs. The alpha-elements-to-oxygen abundance ratios (Ne/O, S/O, Ar/O) are in very good agreement with the mean values for other metal-deficient BCDs and are consistent with the scenario that these elements are made in massive stars. The Fe/O abundance ratio is ~2 times smaller than the solar ratio. The Si/O ratio is close to the solar value, implying that silicon is not significantly depleted into dust grains. The values of the N/O and C/O ratios imply that intermediate-mass stars have not had time to evolve in SBS 1415+437 and release their nucleosynthesis products and that both N and C in the BCD have been made by massive stars only. This sets an upper limit of ~100 Myr on the age of SBS 1415+437. The (V-I) color of the low-surface-brightness component of the galaxy is blue (<0.4 mag) indicative of a very young underlying stellar population. The (V-I) - I color-magnitude diagrams of the resolved stellar populations in different regions of SBS 1415+437 suggest propagating star formation from the NE side of the galaxy to the SW. All regions in SBS 1415+437 possess very blue spectral energy distributions (SED). We find that the ages of the stellar populations in SBS 1415+437 to range from a few Myr to 100 Myr.Comment: 25 pages, 12 PS and 5 JPG figures, to appear in Ap

Improving Predictions for Helium Emission Lines

We have combined the detailed He I recombination model of Smits with the collisional transitions of Sawey & Berrington in order to produce new accurate helium emissivities that include the effects of collisional excitation from both the 2 (3)S and 2 (1) S levels. We present a grid of emissivities for a range of temperature and densities along with analytical fits and error estimates. Fits accurate to within 1% are given for the emissivities of the brightest lines over a restricted range for estimates of primordial helium abundance. We characterize the analysis uncertainties associated with uncertainties in temperature, density, fitting functions, and input atomic data. We estimate that atomic data uncertainties alone may limit abundance estimates to an accuracy of 1.5%; systematic errors may be greater than this. This analysis uncertainty must be incorporated when attempting to make high accuracy estimates of the helium abundance. For example, in recent determinations of the primordial helium abundance, uncertainties in the input atomic data have been neglected.Comment: ApJ, accepte

Factors contributing to high performance of sows in free farrowing systems

\ua9 The Author(s) 2024. Background: Pressure to abolish farrowing crates is increasing, and producers are faced with decisions about which alternative system to adopt. For sow welfare, well designed free farrowing systems without close confinement are considered optimal but producers have concerns about increased piglet mortality, particularly crushing by the sow. Reporting accurate performance figures from commercial farms newly operating such systems could inform the transition process. This study investigated performance on three commercial farms operating four different zero-confinement systems, three of which were newly installed. A total of 3212 litters from 2920 sows were followed from farrowing to weaning over a three-year period with key performance indicators (KPIs) recorded. Mixed Models (LMMs, GLMMs) determined the influence of different factors (e.g. farrowing system, sow parity, management aspects) and litter characteristics on performance, including levels and causes of piglet mortality. Results: Piglet mortality was significantly influenced by farm/system. Live-born mortality ranged from 10.3 to 20.6% with stillbirths ranging from 2.5 to 5.9%. A larger litter size and higher parity resulted in higher levels of mortality regardless of system. In all systems, crushing was the main cause of piglet mortality (59%), but 31% of sows did not crush any piglets, whilst 26% crushed only one piglet and the remaining sows (43%) crushed two or more piglets. System significantly influenced crushing as a percentage of all deaths, with the system with the smallest spatial footprint (m2) compared to the other systems, recording the highest levels of crushing. Time from the start of the study influenced mortality, with significant reductions in crushing mortality (by ~ 4%) over the course of the three-year study. There was a highly significant effect of length of time (days) between moving sows into the farrowing accommodation and sows farrowing on piglet mortality (P &lt; 0.001). The less time between sows moving in and farrowing, the higher the levels of piglet mortality, with ~ 3% increase in total mortality every five days. System effects were highly significant after adjusting for parity, litter size, and days pre-farrowing. Conclusion: These results from commercial farms demonstrate that even sows that have not been specifically selected for free farrowing are able, in many cases, to perform well in these zero-confinement systems, but that a period of adaptation is to be expected for overall farm performance. There are performance differences between the farms/systems which can be attributed to individual farm/system characteristics (e.g. pen design and management, staff expertise, pig genotypes, etc.). Higher parity sows and those producing very large litters provide a greater challenge to piglet mortality in these free farrowing systems (just as they do in crate systems). Management significantly influences performance, and ensuring sows have plenty of time to acclimatise between moving in to farrowing accommodation and giving birth is a critical aspect of improving piglet survival

Planetary Nebula Abundances and Morphology: Probing the Chemical Evolution of the Milky Way

This paper presents a homogeneous study of abundances in a sample of 79 northern galactic planetary nebulae whose morphological classes have been uniformly determined. Ionic abundances and plasma diagnostics were derived from selected optical line strengths in the literature, and elemental abundances were estimated with the Ionization Correction Factor developed by Kingsbourgh & Barlow (1994). We compare the elemental abundances to the final yields obtained from stellar evolution models of low-and intermediate-mass stars, and we confirm that most Bipolar planetary nebulae have high nitrogen and helium abundance, and are the likely progeny of stars with main-sequence mass larger than 3 solar masses. We derive =0.27, and discuss the implication of such a high ratio in connection with the solar neon abundance. We determine the galactic gradients of oxygen and neon, and found Delta log (O/H)/Delta R=-0.01 dex/kpc$ and Delta log (Ne/H)/Delta R=-0.01 dex/kpc. These flat PN gradients do not reconcile with galactic metallicity gradients flattening with time.Comment: The Astrophysical Journal, in pres

The Early Evolution of Massive Stars: Radio Recombination Line Spectra

Velocity shifts and differential broadening of radio recombination lines are used to estimate the densities and velocities of the ionized gas in several hypercompact and ultracompact HII regions. These small HII regions are thought to be at their earliest evolutionary phase and associated with the youngest massive stars. The observations suggest that these HII regions are characterized by high densities, supersonic flows and steep density gradients, consistent with accretion and outflows that would be associated with the formation of massive stars.Comment: ApJ in pres