Intestinal epithelial responses to Salmonella enterica serovar Enteritidis: Effects on intestinal permeability and ion transport

Salmonella infection of chickens that leads to potential human foodborne salmonellosis continues to be a major concern. Chickens serve as carriers but, in contrast to humans, rarely show any clinical signs including diarrhea. The present investigations aimed to elucidate whether the absence of diarrhea during acute Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) infection may be linked to specific changes in the electrophysiological properties of the chicken gut. Immediately after slaughter, intestinal pieces of the mid-jejunum and cecum of either commercial broiler or specific pathogen-free (SPF) chickens were mounted in Ussing chambers in 2 separate experimental series. Living Salmonella Enteritidis (3 × 109) or Salmonella Enteritidis endotoxin (20 mg/L), or both, were added to the mucosal side for 1 h. In both experimental series, the Salmonella infection decreased the trans-epithelial ion conductance Gt (P < 0.05). In the jejunum of SPF chickens, there was also a marked decrease in net charge transfer across the epithelium, evidenced by decreased short-circuit current (Isc, P < 0.05). Interestingly, the mucosal application of Salmonella endotoxin to the epithelial preparations from jejunum and cecum of SPF chicken had an effect similar to living bacteria. However, the endotoxin had no additional effect on the intestinal function in the presence of bacteria. The decreasing effect of Salmonella and or its endotoxin on Gt could be partly reversed by serosal addition of histamine. To our knowledge, this is the first study to address the functional response of native intestinal epithelium of chicken to an in vitro Salmonella infection. For the first time, it can be reported that intestinal ion permeability of chicken decreases acutely by the presence of Salmonella. This type of response could counteract ion and fluid secretion and may thus, at least in part, explain why chickens do not develop overt diarrhea after Salmonella infection

Scale Invariance and the AdS/CFT Correspondence

Using the AdS/CFT correspondence, we show that the Anti-de Sitter (AdS) rotating (Kerr) black holes in five and seven dimensions provide us with examples of non-trivial field theories which are scale, but not conformally invariant. This is demonstrated by our computation of the actions and the stress-energy tensors of the four and six dimensional field theories residing on the boundary of these Kerr-AdS black holes spacetimes.Comment: 3 pages. LaTeX, IJMP style. Contribution to proceedings of DPF 2000, held at Ohio State

Higher Dimensional Taub-NUTs and Taub-Bolts in Einstein-Maxwell Gravity

We present a class of higher dimensional solutions to Einstein-Maxwell equations in d-dimensions. These solutions are asymptotically locally flat, de-Sitter, or anti-de Sitter space-times. The solutions we obtained depend on two extra parameters other than the mass and the nut charge. These two parameters are the electric charge, q and the electric potential at infinity, V, which has a non-trivial contribution. We Analyze the conditions one can impose to obtain Taub-Nut or Taub-Bolt space-times, including the four-dimensional case. We found that in the nut case these conditions coincide with that coming from the regularity of the one-form potential at the horizon. Furthermore, the mass parameter for the higher dimensional solutions depends on the nut charge and the electric charge or the potential at infinity.Comment: 11 pages, LaTe

Campylobacter jejuni colonization promotes the translocation of Escherichia coli to extra-intestinal organs and disturbs the short-chain fatty acids profiles in the chicken gut

For a long time Campylobacter was only considered as a commensal microorganism in avian hosts restricted to the ceca, without any pathogenic features. The precise reasons for the symptomless chicken carriers are still unknown, but investigations of the gastrointestinal ecology of broiler chickens may improve our understanding of the microbial interactions with the host. Therefore, the current studies were conducted to investigate the effects of Campylobacter jejuni colonization on Escherichia coli translocation and on the metabolic end products (short-chain fatty acids, SCFAs). Following oral infection of 14 day old broiler chickens with 1 × 108 CFU of Campylobacter jejuni NCTC 12744 in two independent animal trials, it was found that C. jejuni heavily colonized the intestine and disseminate to extra-intestinal organs. Moreover, in both animal trials, the findings revealed that C. jejuni promoted the translocation of E. coli with a higher number encountered in the spleen and liver at 14 days post infection (dpi). In addition, Campylobacter affected the microbial fermentation in the gastrointestinal tract of broilers by reducing the amount of propionate, isovalerate, and isobutyrate in the cecal digesta of the infected birds at 2 dpi and, at 7 and 14 dpi, butyrate, isobutyrate, and isovalerate were also decreased. However, in the jejunum, the C. jejuni infection lowered only butyrate concentrations at 14 dpi. These data indicated that C. jejuni may utilize SCFAs as carbon sources to promote its colonization in the chicken gut, suggesting that Campylobacter cannot only alter gut colonization dynamics but might also influence physiological processes due to altered microbial metabolite profiles. Finally, the results demonstrated that C. jejuni can cross the intestinal epithelial barrier and facilitates the translocation of Campylobacter itself as well as of other enteric microorganisms such as E. coli to extra-intestinal organs of infected birds. Altogether, our findings suggest that the Campylobacter carrier state in chicken is characterised by multiple changes in the intestinal barrier function, which supports multiplication and survival within the host

Scale without Conformal Invariance at Three Loops

We carry out a three-loop computation that establishes the existence of scale without conformal invariance in dimensional regularization with the MS scheme in d=4-epsilon spacetime dimensions. We also comment on the effects of scheme changes in theories with many couplings, as well as in theories that live on non-conformal scale-invariant renormalization group trajectories. Stability properties of such trajectories are analyzed, revealing both attractive and repulsive directions in a specific example. We explain how our results are in accord with those of Jack & Osborn on a c-theorem in d=4 (and d=4-epsilon) dimensions. Finally, we point out that limit cycles with turning points are unlike limit cycles with continuous scale invariance.Comment: 21 pages, 3 figures, Erratum adde

Higher Dimensional Kerr-AdS Black Holes and the AdS/CFT Correspondence

Using the counterterm subtraction technique we calculatehe stress-energy tensor, action, and other physical quantities for Kerr-AdS black holes in various dimensions. For Kerr-AdS_5 with both rotation parameters non-zero, we demonstrate that stress-energy tensor, in the zero mass parameter limit, is equal to the stress tensor of the weakly coupled four dimensional dual field theory. As a result, the total energy of the generalKerr-AdS_5 black hole at zero mass parameter, exactly matches the Casimir energy of the dual field theory. We show that at high temperature, the general Kerr-AdS_5 and perturbative field theory stress-energy tensors are equal, up to the usual factor of 3/4. We also use the counterterm technique to calculate the stress tensors and actions for Kerr-AdS_6, and Kerr-AdS_7 black holes, with one rotation parameter, and we display the results. We discuss the conformal anomalies of the field theories dual to the Kerr-AdS_5 and Kerr-AdS_7 spacetimes. In these two field theories, we show that the rotation parameters break conformal invariance but not scale invariance, a novel result for a non-trivial field theory. For Kerr-AdS_7 the conformal anomalies calculated on the gravity side and the dual (0,2) tensor multiplet theory are equal up to 4/7 factor. We expect that the Casimir energy of the free field theory is the same as the energy of the Kerr-AdS_7 black hole (with zero mass parameter), up to that factor.Comment: 18 pages, LaTex (v3: references added. footnote added

Conserved Charges in Even Dimensional Asymptotically locally Anti-de Sitter Space-times

Based on the recent paper hep-th/0503045, we derive a formula of calculating conserved charges in even dimensional asymptotically {\it locally} anti-de Sitter space-times by using the definition of Wald and Zoupas. This formula generalizes the one proposed by Ashtekar {\it et al}. Using the new formula we compute the masses of Taub-Bolt-AdS space-times by treating Taub-Nut-AdS space-times as the reference solution. Our result agrees with those resulting from "background subtraction" method or "boundary counterterm" method. We also calculate the conserved charges of Kerr-Taub-Nut-AdS solutions in four dimensions and higher dimensional Kerr-AdS solutions with Nut charges. The mass of (un)wrapped brane solutions in any dimension is given.Comment: Latex, 28 pages, v2: minor changes, to appear in JHE