Tramtrack Is Genetically Upstream of Genes Controlling Tracheal Tube Size in Drosophila

The Drosophila transcription factor Tramtrack (Ttk) is involved in a wide range of developmental decisions, ranging from early embryonic patterning to differentiation processes in organogenesis. Given the wide spectrum of functions and pleiotropic effects that hinder a comprehensive characterisation, many of the tissue specific functions of this transcription factor are only poorly understood. We recently discovered multiple roles of Ttk in the development of the tracheal system on the morphogenetic level. Here, we sought to identify some of the underlying genetic components that are responsible for the tracheal phenotypes of Ttk mutants. We therefore profiled gene expression changes after Ttk loss- and gain-of-function in whole embryos and cell populations enriched for tracheal cells. The analysis of the transcriptomes revealed widespread changes in gene expression. Interestingly, one of the most prominent gene classes that showed significant opposing responses to loss- and gain-of-function was annotated with functions in chitin metabolism, along with additional genes that are linked to cellular responses, which are impaired in ttk mutants. The expression changes of these genes were validated by quantitative real-time PCR and further functional analysis of these candidate genes and other genes also expected to control tracheal tube size revealed at least a partial explanation of Ttk's role in tube size regulation. The computational analysis of our tissue-specific gene expression data highlighted the sensitivity of the approach and revealed an interesting set of novel putatively tracheal genes

An algorithm for identifying career lines from job history data

A comparatively simple method of uncovering the deep structure of career lines from job history data is described. Key decisions in the construction of the programming algorithm are related to substantive and theoretical ideas. Illustrations of the use of the method are offered, based on blue collar job histories from an American and a Dutch firm. Advantages, drawbacks and other applications of the program are discussed

New skills for new classrooms: training tutors to teach languages online

While online teaching in post-compulsory education is the focus of much research today, the training of online tutors has been largely neglected. Most papers do not go beyond dealing with the technical skills that are needed to teach in an online environment. This article outlines a framework for tutor training, starting with a brief overview of benefits and challenges for online language tutors. On the basis of several years’ experience with teaching languages using a synchronous online environment and training tutors for online language courses, we suggest a pyramid of skills necessary for successful online teaching. These include the more general skills of dealing with the technology and using its advantages, the social skills of community building, language teaching skills, and the skills to teach creatively and develop a personal teaching style in an online medium. The article then suggests how these skills can be implemented in a training programme, which includes both pre-course training and on-going staff development