Determination of Resistance Reference Parameters of Equine Strongyls to Anthelmintic

The research undertaken during November 2008 - April 2009 was aimed to find the required pharmacological reference parameters in order to diagnosis the resistance phenomenon of equine strongyls. Strongyls eggs originated from a total of 126 faecal samples collected during the 6 months of study on three different areas of the Danube Delta, where it is estimated that there are a number of over 4,000 wild untreated equine. Testing effectiveness of Mebendazole (MBZ), Fenbendazole (FBZ) and Ivermectine (IVM) was performed in vitro by larva development assay (LDA). To Fenbendazole the reference lethal concentrations were LC50 0.0089µg/ml; LC90 -0.7430µg/ml and LC100 -0.9265µg/ml with a MIC of -18.6031µg/ml. To Mebendazole the reference lethal concentrations were LC50 -0.0078µg/ml, LC90 -0.4566µg/ml and LC100 -0.5688µg/ml with a MIC of 21.4542µg/ml. To Ivermectine the reference parameters were LC50 -0.00028µg/ml, LC90 0.0011µg/ml and LC100 0.0013µg/ml with a MIC of -250.004µg/ml

Testing some etracts of plants from the Danube Delta with potential antiparasitic effect on equines

The study was done during november 2016 on strongyls eggs from feces from a total of 28 horses in Tulcea county, C.A. Rosetti locality in the Danube Delta. To determine the degree of infestation was used the method McMaster and Stoll (adapted for quantitative larvohelmintology). The tests of hatching eggs (egg hatching assay - EHA) and larval development (larvae development assay - LDA) were made, using as active substances herbal decoctions and hydro-alcoholic solutions made from well known anthelmintic plants. One decoction and two hydro-alcoholic extracts were tested, Hippophae rhamnoides (sea buckthorn) respectively Thymus serpyllum (creeping thyme) and Arthemisia absinthium (wormwood). In order to achieve the two tests for therapeutic efficacy of the tested products were obtained six serial dilutions with concentrations of 50%, 25%, 12.5%, 6.25%, 3.12%, and 1, 56%. For decoctions were made control samples with distilled water, and with alcohol for hydroalcoholic solutions. The data obtained in both tests were analyzed using Anthelmintic Rezistance Program (ARP), determining the parameters "a" and "b" to trace the line prediction and lethal concentrations (CL). We can be concluded that for the decoction was observed an emphatic decrease of the percentage of larval development. For the two hydro-alcoholic extracts the hatching percentage of Strongyls eggs was different depending on the concentration used, the maximum value being registered at 25% dilution, in which the percentage of hatching was 82.35%, and the minimum concentration of 12.50%. LDA test showed a significant reduction in the percentage of larval Strongyls development, stage 3, at all dilutions, which is less than 50%. Ke