Differential basolateral–apical distribution of scavenger receptor, class B, type I in cultured cells and the liver

The high-density lipoprotein (HDL) receptor, scavenger receptor class B, type I (SR-BI), mediates selective cholesteryl ester uptake into the liver, which finally results in cholesterol secretion into the bile. Despite several reports, the distribution of hepatic SR-BI between the sinusoidal and canalicular membranes is still under debate. We present immunohistological data using specific markers showing that the bulk of SR-BI is present in sinusoidal membranes and, to a lesser extent, in canalicular membranes in murine and human liver sections. In addition, SR-BI was detected in preparations of rat liver canalicular membranes. We also compared the in vivo findings to HepG2 cells, a widely used in vitro hepatocyte model. Interestingly, SR-BI was enriched in bile canalicular-like (BC-like) structures in polarized HepG2 cells, which were cultivated either conventionally to form a monolayer or in Matrigel to form three-dimensional structures. Fluorescently labeled HDL was transported into close proximity of BC-like structures, whereas HDL labeled with the fluorescent cholesterol analog BODIPY-cholesterol was clearly detected within these structures. Importantly, similarly to human and mouse liver, SR-BI was localized in basolateral membranes in three-dimensional liver microtissues from primary human liver cells. Our results demonstrate that SR-BI is highly enriched in sinusoidal membranes and is also found in canalicular membranes. There was no significant basolateral–apical redistribution of hepatic SR-BI in fasting and refeeding experiments in mice. Furthermore, in vitro studies in polarized HepG2 cells showed explicit differences as SR-BI was highly enriched in BC-like structures. These structures are, however, functional and accumulated HDL-derived cholesterol. Thus, biological relevant model systems should be employed when investigating SR-BI distribution in vitro. Electronic supplementary material The online version of this article (doi:10.1007/s00418-014-1251-9) contains supplementary material, which is available to authorized users

Spatial fragmentation of a Bose-Einstein condensate in a double-well potential

We present a theoretical study of the ground state of a Bose-Einstein condensate with repulsive inter-particle interactions in a double-well potential, using a restricted variational principle. Within such an approach, there is a transition from a single condensate to a fragmented condensate as the strength of the central barrier of the potential is increased. We determine the nature of this transition through approximate analytic as well as numerical solutions of our model in the regime where the inter-particle interactions can be treated perturbatively. The degree of fragmentation of the condensate is characterized by the degrees of first-order and second-order spatial coherence across the barrier.Comment: 10 pages, 2 figures, submitted to Phys. Rev.

Variety of idempotents in nonassociative algebras

In this paper, we study the variety of all nonassociative (NA) algebras from the idempotent point of view. We are interested, in particular, in the spectral properties of idempotents when algebra is generic, i.e. idempotents are in general position. Our main result states that in this case, there exist at least $n-1$ nontrivial obstructions (syzygies) on the Peirce spectrum of a generic NA algebra of dimension $n$. We also discuss the exceptionality of the eigenvalue $\lambda=\frac12$ which appears in the spectrum of idempotents in many classical examples of NA algebras and characterize its extremal properties in metrised algebras.Comment: 27 pages, 1 figure, submitte

Phospho-regulation of the Shugoshin - Condensin interaction at the centromere in budding yeast.

Correct bioriented attachment of sister chromatids to the mitotic spindle is essential for chromosome segregation. In budding yeast, the conserved protein shugoshin (Sgo1) contributes to biorientation by recruiting the protein phosphatase PP2A-Rts1 and the condensin complex to centromeres. Using peptide prints, we identified a Serine-Rich Motif (SRM) of Sgo1 that mediates the interaction with condensin and is essential for centromeric condensin recruitment and the establishment of biorientation. We show that the interaction is regulated via phosphorylation within the SRM and we determined the phospho-sites using mass spectrometry. Analysis of the phosphomimic and phosphoresistant mutants revealed that SRM phosphorylation disrupts the shugoshin-condensin interaction. We present evidence that Mps1, a central kinase in the spindle assembly checkpoint, directly phosphorylates Sgo1 within the SRM to regulate the interaction with condensin and thereby condensin localization to centromeres. Our findings identify novel mechanisms that control shugoshin activity at the centromere in budding yeast

Output of a pulsed atom laser

We study the output properties of a pulsed atom laser consisting of an interacting Bose-Einstein condensate (BEC) in a magnetic trap and an additional rf field transferring atoms to an untrapped Zeeman sublevel. For weak output coupling we calculate the dynamics of the decaying condensate population, of its chemical potential and the velocity of the output atoms analytically.Comment: 4 pages, RevTeX. Full ps file available on http://mpqibmr1.mpq.mpg.de:5000/~man

Dynamics of Macroscopic Wave Packet Passing through Double Slits: Role of Gravity and Nonlinearity

Using the nonlinear Schroedinger equation (Gross-Pitaevskii equation), the dynamics of a macroscopic wave packet for Bose-Einstein condensates falling through double slits is analyzed. This problem is identified with a search for the fate of a soliton showing a head-on collision with a hard-walled obstacle of finite size. We explore the splitting of the wave packet and its reorganization to form an interference pattern. Particular attention is paid to the role of gravity (g) and repulsive nonlinearity (u_0) in the fringe pattern. The peak-to-peak distance in the fringe pattern and the number of interference peaks are found to be proportional to g^(-1/2) and u_0^(1/2)g^(1/4), respectively. We suggest a way of designing an experiment under controlled gravity and nonlinearity.Comment: 10 pages, 4 figures and 1 tabl

Interference of Bose-Einstein condensates in momentum space

We suggest an experiment to investigate the linear superposition of two spatially separated Bose-Einstein condensates. Due to the coherent combination of the two wave functions, the dynamic structure factor, measurable through inelastic photon scattering at high momentum transfer $q$, is predicted to exhibit interference fringes with frequency period $\Delta\nu = q/md$ where $d$ is the distance between the condensates. We show that the coherent configuration corresponds to an eigenstate of the physical observable measured in the experiment and that the relative phase of the condensates is hence created through the measurement process.Comment: 4 pages and 2 eps figure

Raman Topography and Strain Uniformity of Large-Area Epitaxial Graphene

We report results from two-dimensional Raman spectroscopy studies of large-area epitaxial graphene grown on SiC. Our work reveals unexpectedly large variation in Raman peak position across the sample resulting from inhomogeneity in the strain of the graphene film, which we show to be correlated with physical topography by coupling Raman spectroscopy with atomic force microscopy. We report that essentially strain free graphene is possible even for epitaxial graphene.Comment: 10 pages, 3 figure

Spatial coherence and density correlations of trapped Bose gases

We study first and second order coherence of trapped dilute Bose gases using appropriate correlation functions. Special attention is given to the discussion of second order or density correlations. Except for a small region around the surface of a Bose-Einstein condensate the correlations can be accurately described as those of a locally homogeneous gas with a spatially varying chemical potential. The degrees of first and second order coherence are therefore functions of temperature, chemical potential, and position. The second order correlation function is governed both by the tendency of bosonic atoms to cluster and by a strong repulsion at small distances due to atomic interactions. In present experiments both effects are of comparable magnitude. Below the critical temperature the range of the bosonic correlation is affected by the presence of collective quasi-particle excitations. The results of some recent experiments on second and third order coherence are discussed. It is shown that the relation between the measured quantities and the correlation functions is much weaker than previously assumed.Comment: RevTeX, 25 pages with 7 Postscript figure