11 research outputs found
Blender file containing the complete virtual anatomical atlas of the Platynereis eye circuitry
Simplified 3D models of all neurons, synapses, muscles and ciliated cells are included. The file can be viewed with the Blender software http://www.blender.org/
Average full-body gene expression patterns of r-opsin1, hdc and VAChT
RGB tiff stack of average registered full-body gene expression patterns of eye marker gene r-opsin1 (red) and neurotransmitter marker genes hdc (green) and VAChT (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands, parapodia and chaetae of larvae is background
acetylated tubulin staining registered to the unbiased nuclear reference
Tiff stack of average acetylated tubulin staining registered to the unbiased nuclear reference of 3-day-old Platynereis larvae. The antibody labels cilia and the axonal scaffold (white)
Double in situ hybridization for r-opsin1 and hdc
RGB tiff stack of raw data from high-resolution apical scan of double RNA whole mount in situ hybridization with r-opsin1 (red) and hdc (green) in 3-day-old Platynereis larvae. Larva is counterstained with acetylated tubulin antibody (blue) to show cilia and axonal scaffold
Phototaxis videos of control and mecamylamine-treated larvae
Videos of phototaxis experiments. 3-day-old control larvae and larvae treated with 50 渭m mecamylamine were imaged with 15 frames per second in a split phototaxis assay cuvette. The length of the cuvette is 15 mm. Directional white stimulus light was provided from the left. Each video is 600 frames. The videos are in Zeiss zvi format. The files were compressed with tar -jcvf archive_name.tar.bz2 directory_with_files/*, use tar 鈥搄xvf to uncompress them
Average full-body gene expression patterns of gad, TyrH and dbh
RGB tiff stack of average registered full-body gene expression patterns of neurotransmitter marker genes gad (red), TyrH (green) and dbh (blue), created by RNA whole mount in situ hybridization in 3-day-old Platynereis larvae. Note that signal present in spinning glands and chaetae of larvae is background, however, signal in the parapodia in the TyrH (green) channel represents real gene expression
Phototaxis ImageJ Macro
ImageJ macro used to score phototaxis videos
Node ranking in the Platynereis eye circuitry
Node ranking in the Platynereis eye circuitry based on various centrality measures
Double in situ hybridization for r-opsin1 and hdc
RGB tiff stack of raw data from high-resolution apical scan of double RNA whole mount in situ hybridization with r-opsin1 (red) and hdc (green) in 3-day-old Platynereis larvae. Larva is counterstained with acetylated tubulin antibody (blue) to show cilia and axonal scaffold
High-resolution image stack of the Platynereis larval primary optic neuropil
High-resolution (1.13 nm/pixel) image stack (tiff series) of the Platynereis larval primary optic neuropil. Contains 4 layers of 22,304 x 5680 pixels