Weaning and Supplementation Increase Liveweight Gain of Bali (Bos javanicus) Cattle of Small-holder Farmers in Central Lombok, Indonesia

Abstract. One of the constraints for improving productivity of Bali cattle in west Nusa Tenggara Province is the low growth rate of weaned calves. Results of on-station experiments showed that Sesbania grandiflora (sesbania) supplementation can significantly increase live weight gain of weaned calves. The objective of this experiment was to investigate whether sesbania supplementation can increase live weight gain of weaned calves and improve farmer income. This experiment was carried out in Tandek, Labulia Village (treatment village) and in Kelebuh and Sukaraja villages (control villages) central Lombok District from April to October 2008. A total of 21 calves (9 males and 12 females) aged 237±7 days with initial live weight of 122.3±4.5 kg were monitored in Kelebuh and Sukaraja villages (control villages). During the same period, 25 calves (13 males and 12 females) aged 237±6 days with initial weight of  108.8±4.1 kg were monitored in Tandek Village (treatment village) and fed fresh sesbania at 1% dry matter of live weight. To ensure that sesbania was fed at the recommended level, regular weighing of sesbania offered by each farmer was conducted 2-3 times per week. All calves were weighed every month to determine live weight gain. Farmers from the control villages and other villages were invited to a field day conducted in the treatment village at the end of the experiment. The results showed that sesbania supplementation significantly increased live weight gain (0.36±0.01 kg/day for calves in the treatment village compared to 0.22±0.01 kg/day for those in the control villages), increased body condition and health of the calves and improved farmer income. Weaning management and supplementation with sesbania was very easy to implement so most of the farmers participated in this study stated that they were willing to continue this feeding management.Keywords: Bali calves, growth, early weaning, Sesbania grandiflora, LombokAbstrak. Salah satu permasalahan untuk meningkatkan produktivitas sapi Bali di NTB adalah rendahnya tingkat pertumbuhan pedet lepas sapih. Hasil kajian di pusat penelitian menunjukkan bahwa suplementasi dengan pakan lokal seperti daun turi terbukti dapat meningkatkan pertambahan bobot badan pedet lepas sapih secara nyata. Penelitian ini bertujuan untuk menguji apakah suplementasi dengan daun turi pada peternakan skala kecil dapat mempercepat pertumbuhan pedet lepas sapih dan meningkatkan pendapatan peternak. Penelitian dilakukan di Dusun Tandek, desa Labulia Lombok Tengah (lokasi intervensi) dan di Desa Kelebuh dan Sukaraja Kecamatan Praya Tengah (lokasi kontrol), Kabupaten Lombok Tengah mulai bulan April sampai Oktober 2008. Sejumlah 21 ekor pedet (9 jantan dan 12 betina) yang berumur 237±7 hari dengan bobot badan awal 122,3±4,5 kg diamati di Desa Kelebuh dan Sukaraja (lokasi kontrol). Pada saat yang sama, 25 ekor pedet (13 jantan dan 12 betina) berumur 237±6 hari dengan bobot badan awal 108,8±4,1 kg diamati di Tandek (lokasi intervensi) dan diberikan pakan tambahan berupa daun turi (sekitar 1% BK dari bobot badannya). Untuk memastikan jumlah daun turi diberikan sesuai dengan rekomendasi, dilakukan penimbangan daun turi yang diberikan secara acak dua sampai 3 kali seminggu. Penimbangan bobot badan dilakukan setiap bulan dan pada akhir penelitian dilakukan temu lapang dengan mengundang peternak dari lokasi kontrol dan lokasi lainnya di Lombok Tengah. Hasil penelitian menunjukkan bahwa penyapihan dan suplementasi daun turi secara nyata meningkatkan pertambahan bobot badan lepas sapih (0,36±0,01 kg/hari pada kelompok intervensi dibandingkan dengan 0,22±0,01 kg/hari pada lokasi kontrol), meningkatkan kondisi dan kesehatan pedet dan meningkatkan keuntungan peternak. Manajemen penyapihan dan suplementasi dengan daun turi sangat mudah dilakukan sehingga sebagian besar peternak yang berpartisipasi dalam temu lapang menyatakan bersedia menerapkannya.Kata kunci : Sapi Bali, pertumbuhan, penyapihan, Sesbania grandiflora, Lombok Dahlanuddin et al/Animal Production 14(3):173-179, September 201

Paracrine-mediated neuroprotection and neuritogenesis of axotomised retinal ganglion cells by human dental pulp stem cells:Comparison with human bone marrow and adipose-derived mesenchymal stem cells

We have investigated and compared the neurotrophic activity of human dental pulp stem cells (hDPSC), human bone marrow-derived mesenchymal stem cells (hBMSC) and human adipose-derived stem cells (hAMSC) on axotomised adult rat retinal ganglion cells (RGC) in vitro in order to evaluate their therapeutic potential for neurodegenerative conditions of RGC. Using the transwell system, RGC survival and length/number of neurites were quantified in coculture with stem cells in the presence or absence of specific Fc-receptor inhibitors to determine the role of NGF, BDNF, NT-3, VEGF, GDNF, PDGF-AA and PDGF-AB/BB in stem cell-mediated RGC neuroprotection and neuritogenesis. Conditioned media, collected from cultured hDPSC/hBMSC/hAMSC, were assayed for the secreted growth factors detailed above using ELISA. PCR array determined the hDPSC, hBMSC and hAMSC expression of genes encoding 84 growth factors and receptors. The results demonstrated that hDPSC promoted significantly more neuroprotection and neuritogenesis of axotomised RGC than either hBMSC or hAMSC, an effect that was neutralized after the addition of specific Fc-receptor inhibitors. hDPSC secreted greater levels of various growth factors including NGF, BDNF and VEGF compared with hBMSC/hAMSC. The PCR array confirmed these findings and identified VGF as a novel potentially therapeutic hDPSC-derived neurotrophic factor (NTF) with significant RGC neuroprotective properties after coculture with axotomised RGC. In conclusion, hDPSC promoted significant multi-factorial paracrine-mediated RGC survival and neurite outgrowth and may be considered a potent and advantageous cell therapy for retinal nerve repair

Sexual practices among unmarried adolescents in Tanzania

\ud Sexual activities are increasingly changing from the cultural point of view what they used to be. Knowledge of these practices among adolescents may be a basis to create awareness among adolescents on practices that involve risks. This study aims to assess sexual practices among unmarried adolescents in Tanzania. A cross-sectional survey was conducted among in-school and out-of-school but unmarried adolescents aged 10 to 19 in five locations in Tanzania. A questionnaire was used to collect information and to characterize sexual practices among these adolescents. About 32% of adolescents reported being sexually active; a higher proportion being males than females. The only inquired and reported sexual practices include vaginal sex, masturbation, oral and anal sex. About 15% of sexually active adolescents reported having multiple sexual partners. Significantly more males reported having multiple partners than females. Nearly 42% of sexually active adolescents reported having used a condom during most recent sexual act. Females reported older partners at first sexual act. Adolescents experience several sexual practices that include penetrative and non-penetrative. More males reported being sexually active than females. Despite adolescents reporting having multiple sexual partners, reported condom use during the most recent sexual act was low. We advocate for a more enhanced approach of reproductive health education that includes safer sex to adolescents without forgetting those in-schools.\u

Effectiveness of cricoid pressure in preventing gastric aspiration during rapid sequence intubation in the emergency department: study protocol for a randomised controlled trial

<p>Abstract</p> <p>Background</p> <p>Cricoid pressure is considered to be the gold standard means of preventing aspiration of gastric content during Rapid Sequence Intubation (RSI). Its effectiveness has only been demonstrated in cadaveric studies and case reports. No randomised controlled trials comparing the incidence of gastric aspiration following emergent RSI, with or without cricoid pressure, have been performed. If improperly applied, cricoid pressure increases risk to the patient. The clinical significance of aspiration in the emergency department is unknown. This randomised controlled trial aims to; 1. Compare the application of the 'ideal" amount of force (30 - 40 newtons) to standard, unmeasured cricoid pressure and 2. Determine the incidence of clinically defined aspiration syndromes following RSI using a fibrinogen degradation assay previously described.</p> <p>Methods/design</p> <p>212 patients requiring emergency intubation will be randomly allocated to either control (unmeasured cricoid pressure) or intervention groups (30 - 40 newtons cricoid pressure). The primary outcome is the rate of aspiration of gastric contents (determined by pepsin detection in the oropharyngeal/tracheal aspirates or treatment for aspiration pneumonitis up to 28 days post-intubation). Secondary outcomes are; correlation between aspiration and lowest pre-intubation Glasgow Coma Score, the relationship between detection of pepsin in trachea and development of aspiration syndromes, complications associated with intubation and grade of the view on direct largyngoscopy.</p> <p>Discussion</p> <p>The benefits and risks of cricoid pressure application will be scrutinised by comparison of the incidence of aspiration and difficult or failed intubations in each group. The role of cricoid pressure in RSI in the emergency department and the use of a pepsin detection as a predictor of clinical aspiration will be evaluated.</p> <p>Trial registration</p> <p>Australian New Zealand Clinical Trials Registry (ANZCTR): <a href="http://www.anzctr.org.au/ACTRN12611000587909.aspx">ACTRN12611000587909</a></p

Two Coregulated Efflux Transporters Modulate Intracellular Heme and Protoporphyrin IX Availability in Streptococcus agalactiae

Streptococcus agalactiae is a major neonatal pathogen whose infectious route involves septicemia. This pathogen does not synthesize heme, but scavenges it from blood to activate a respiration metabolism, which increases bacterial cell density and is required for full virulence. Factors that regulate heme pools in S. agalactiae are unknown. Here we report that one main strategy of heme and protoporphyrin IX (PPIX) homeostasis in S. agalactiae is based on a regulated system of efflux using two newly characterized operons, gbs1753 gbs1752 (called pefA pefB), and gbs1402 gbs1401 gbs1400 (called pefR pefC pefD), where pef stands for ‘porphyrin-regulated efflux’. In vitro and in vivo data show that PefR, a MarR-superfamily protein, is a repressor of both operons. Heme or PPIX both alleviate PefR-mediated repression. We show that bacteria inactivated for both Pef efflux systems display accrued sensitivity to these porphyrins, and give evidence that they accumulate intracellularly. The ΔpefR mutant, in which both pef operons are up-regulated, is defective for heme-dependent respiration, and attenuated for virulence. We conclude that this new efflux regulon controls intracellular heme and PPIX availability in S. agalactiae, and is needed for its capacity to undergo respiration metabolism, and to infect the host

The Kolumbo submarine volcano of Santorini island is a large pool of bacterial strains with antimicrobial activity

Microbes in hydrothermal vents with their unique secondary metabolism may represent an untapped potential source of new natural products. In this study, samples were collected from the hydrothermal field of Kolumbo submarine volcano in the Aegean Sea, in order to isolate bacteria with antimicrobial activity. Eight hundred and thirty-two aerobic heterotrophic bacteria were isolated and then differentiated through BOX-PCR analysis at the strain level into 230 genomic fingerprints, which were screened against 13 different type strains (pathogenic and nonpathogenic) of Gram-positive, Gram-negative bacteria and fungi. Forty-two out of 176 bioactive-producing genotypes (76 %) exhibited antimicrobial activity against at least four different type strains and were selected for 16S rDNA sequencing and screening for nonribosomal peptide (NRPS) and polyketide (PKS) synthases genes. The isolates were assigned to genus Bacillus and Proteobacteria, and 20 strains harbored either NRPS, PKS type I or both genes. This is the first report on the diversity of culturable mesophilic bacteria associated with antimicrobial activity from Kolumbo area; the extremely high proportion of antimicrobial-producing strains suggested that this unique environment may represent a potential reservoir of novel bioactive compounds