Pengaruh Pemberian Vitamin E Terhadap Kualitas Spermatozoa Mencit Jantan (Mus Musculus) Yang Diberi Paparan Suhu

: Vitamin E is an antioxidant for two classes of molecule, namely Tocopherol and Tocotrienol that have role in body nutrient. Purpose of this study was to determine the effect and mechanism of vitamin E on the quality of spermatozoa of mice. This study used a descriptive observational method. The subject of the study consist of 27 mice were divided into 3 groups: group P0 not given temperature exposure and vitamin E, P1 groups given exposure to temperatures of 40°C, P2 groups given exposure to temperatures of 40°C and vitamin E. Treatment carried out for 32 days. The results of research on the P2 group showed improvement in the quality of spermatozoa with a mean concentration of spermatozoa (68.7 x105mL), the mean percentage of normal spermatozoa morphology (61%), the mean percentage of abnormal spermatozoa morphology (39%), the mean percentage spermatozoa motility Ma (38.1%). Conclusion: sperm quality can be improved with vitamin E after exposure to hot temperatures

Perbedaan Motilitas Spermatozoa Sapi Janta (Frisian Holstein) Setelah Pemberian Cairan Kristaloid-ringer Laktat

:Spermatozoa motility is one of the benchmarks determining Fertilisation, where energy is used for movement is influenced by energy sources such as fructose, and liquid electrolytes such as Na +, Ca +, Cl-, and lactate.This study aimed to assess the motility of spermatozoa after crystalloid fluid penembahan given Ringer's lactate. The design of this study is the correlation study with a sample of 4 bull testiclesData collected by differentiating cauda epididymis of bull testicles and Ringer's lactate given crystalloid fluid is then observed under a microscope. From the research that has been done on the motility of the results obtained after administration of Ringer's lactate infusion at 56.35% while the non-provision of 52.5%. Conclusion: The bull sperm have a progressive motility after having given crystalloid fluid Ringer's lactate

Pengaruh Pemberian Ekstrak Kulit Buah Manggis (Garcinia Mangostana L.) Terhadap Kualitas Spermatozoa Wistar Jantan (Rattus Norvegicus) Yang Diberi Paparan Suhu Panas

: High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Mangosteen rind contains polyphenols inter alia xanthones which have antioxidant properties. As free radical scavengers, the xanthones provide fast hydrogen atom movement into the lipid radicals and turn them into more stable forms. This study aimed to determine the effect of extract of mangosteen rind on wistar male sperm quality exposed to heat. This was an experimental study with a posttest randomized control group design. Samples were 6 wistar rats divided into 2 groups: group P0 was exposured to 400C of temperature; group P1 was exposured to the same temperature however the rats were given extract of mangosteen rind. The treatment was done for 52 days. The results showed that P1 group showed some improvement in the quality of spermatozoa with an average concentration of spermatozoa 76.5, x 106 mL, average percentage of motility 82.2%, average percentage of morphologically normal spermatozoa 81.7%, and average percentage of abnormal morphology 18.3%. Conclusion: Extract of mangosteen rind can increase and improve the spermatozoa quality (concentration, motility, and morphology) of wistar rats exposed to heat

Uji Bioaktivitas Ekstrak Padina Australis Dari Pesisir Pantai Molas Sulawesi Utara Terhadap Bakteri Staphylococcus Epidermidis

Penelitian ini bertujuan untuk mengetahui bioaktivitas ekstrak P. australis terhadap bakteri S. epidermidis. Metode yang digunakan dalam uji bioaktivitas adalah uji Minimum Inhibitory Concentration (MIC) dan uji Minimum Bactericidal Concentration (MBC). Pengujian bioaktivitas dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu seri konsentrasi ekstrak 30%, 60%, 90%, kontrol negatif (CMC 1%) dan kontrol positif (cotrimoksazole). Penentuan nilai MIC dengan analisis kekeruhan menggunakan spektrofotometer (ƛ630 nm) sedangkan nilai MBC dengan metode pour plate. Data hasil uji MBC dianalisis menggunakan ANOVA one way kemudian dilanjutkan dengan Uji Tukey. Hasil penelitian ini menunjukkan nilai MIC (Kadar Hambat Minimal) adalah konsentrasi 90%. Hasil analisa statistika ANOVA one way data uji MBC menunjukkan terdapat perbedaan yang signifikan antara pemberian ekstrak dengan total koloni yang tumbuh pada media NA. Uji Tukey menunjukkan bahwa ekstrak P. australis yang menunjukkan bioaktivitas terbaik terhadap S. epidermidis adalah konsentrasi 90% dengan selisih 631 koloni dengan konsentrasi 60% dan 658 koloni dengan konsentrasi 30%. Total koloni yang tumbuh pada media NA dengan perlakuan konsentrasi ekstrak 90% adalah 31 koloni. Ekstrak etanol Padina australis memiliki bioaktivitas dalam menghambat pertumbuhan bakteri S. epidermidis. Nilai MIC ekstrak P. australis terhadap bakteri S. epidermidis adalah konsentrasi 90%. Nilai MBC tidak diketahui dikarenakan pada pengujian lanjut (MBC) konsentrasi yang menunjukkan nilai MIC merupakan konsentrasi tertinggi yaitu 90% masih ditemukan koloni bakteri yang tumbuh pada media N

Pola Aktivitas Harian Tangkasi (Tarsius Spectrum) Di Taman Marga Satwa Naemundung Kota Bitung

Tangkasi (Tarsius spectrum) adalah primata primitif dari Famili Tarsidae dan merupakan primata endemik di Sulawesi. Tangkasi memiliki tubuh kecil, mempunyai mata bulat besar, dapat melompat dan dapat membalik 180˚. Karena keunikan yang dimiliki hewan ini menjadikannya disukai banyak orang sehingga diburu, diperdagangkan secara illegal dan dijadikan sebagai hewan peliharaan. Padahal tangkasi (T. spectrum) dilindungi, termasuk kategori rentan (Vulnerable) dan tercantum dalam CITES Appendix II. Tangkasi yang telah dikandangkan akan mengalami Perubahan perilaku dibanding yang ada di alam. Penelitian aktivitas harian tangkasi yang ada di dalam kandang belum pernah dilakukan di Sulawesi Utara, oleh karena itu, maka penelitian ini perlu dilakukan. Penelitian yang menganalisis aktivitas harian dan tingkah laku tangkasi (T. spectrum) di dalam kandang khususnya dilihat dari aktivitas makan, mencari makan, beristirahat, berpindah, dan aktivitas sosial. Metode pengambilan data secara Instantaneous sampling. Hasil pengamatan terhadap pola aktivitas harian di kandang yaitu : makan (1,5%), mencari makan (8,5%), berpindah (26,8%), instirahat (57,5%), dan sosial (5,5%). Aktivitas tertinggi adalah istirahat, diikuti dengan aktivitas berpindah, kemudian mencari makan, sosial dan yang terendah adalah aktivitas makan.Tangkasi (Tarsius spectrum) is a primitive primate from Family Tarsidae and a endemic primate in Sulawesi. Tangkasi have a small body, and ayeball, they can jump and their can head flipped until 180˚. Tangkasi already caged will experience a change in behavior campared to the wild. Many people hunt, trade illegally and use it as pet because of the uniqueness of this species. Even though tangkasi (T. spectrum) is protected, included in vulnerable category and listed in CITES Appendix II. Research on daily activity of tangkasi in the cage has never been done in North Sulawesi, therefore, this research needs to be done. Studies analyzing daily activities and behavior tangkasi (T. spectrum) in the cage in terms of the activity of eating, foraging, resting, moving, and social activities. Data were collected by using Instantaneous sampling method. The observation of the daily activity patterns in the cage namely: eating (1.5%), foraging (8.5%), moving (26.8%), resting (57.5%), and social (5.5%) . The highest activity is resting, followed by moving activity, foraging, social and respectively the lowest is feeding activity