Canine \u3b2-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs

Canine leishmaniasis caused by Leishmania infantum is a parasitic disease of great veterinary significance. Some dogs infected by L. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the parasite and develop an overt disease, which may be fatal, if left untreated. The initial factors triggering the polarization of the immune response towards a predominantly T-helper 1 or T-helper 2 cytokines, as well as the markers of resistance and susceptibility to L. infantum infection and disease development in dogs, are not fully understood. Herein, we assessed the association between single nucleotide polymorphisms (SNPs) in the canine \u3b2-defensin-1 (CBD1) gene and the infection by L. infantum in two dog populations from Brazil (Sobral in Cear\ue1 State and S\ue3o Raimundo Nonato in Piau\ued State) and one dog population from Italy

The pgm locus and pigmentation phenotype in Yersinia pestis

The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus

Flebotomíneos (Diptera: Psychodidae: Phlebotominae) no Estado de Pernambuco

Submitted by Nuzia Santos ([email protected]) on 2012-09-27T12:39:37Z No. of bitstreams: 1 32.2010.pdf: 551686 bytes, checksum: 7193f3528ec325d9659494c9e95bdd97 (MD5)Made available in DSpace on 2012-09-27T12:39:37Z (GMT). No. of bitstreams: 1 32.2010.pdf: 551686 bytes, checksum: 7193f3528ec325d9659494c9e95bdd97 (MD5) Previous issue date: 2010University of Bari . Department of Public Health and Zootechny, , Valenzano, Bari, Italy/ Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Departamento de Imunologia. Recife, PE, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Pernambuco. Departamento de Genética, Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Referência Nacional e Internacional de Flebotomíneos. Belo Horizonte, MG, BrasilUniversidade Federal de Pernambuco. Departamento de Genética, Recife, PE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhaes. Departamento de Imunologia. Recife, PE, BrasilIntrodução: Flebotomíneos são pequenos insetos de grande importância médica e veterinária. Este artigo apresenta uma lista atualizada dos flebotomíneos que ocorrem no Estado de Pernambuco, Brasil. Métodos: Uma revisão da literatura publicada desde a década de 40 até maio de 2010 foi realizada e material testemunho depositado em coleções de referência foi estudado. Resultados: Um total de 37 espécies de flebotomíneos foram previamente relatadas como presentes em Pernambuco, mas o registro de seis delas necessita de confirmação. Conclusões: Esse trabalho fornece uma lista atualizada de flebotomíneos de Pernambuco, com algumas notas sobre a sua taxonomia, ecologia, distribuição e relevância epidemiológica.Introduction: Phlebotomine sand flies are small insects of great medical relevance. This article presents an updated list of the phlebotomine sand flies occurring in the State of Pernambuco, Brazil. Methods: A review of literature published since the 1940s up to May 2010 was conducted and voucher material deposited in reference collections were studied. Results: A total of 37 phlebotomine species have previously been reported as occurring in Pernambuco, but the record of six species needs confirmation. Conclusions: This work provides an updated list of phlebotomine sand flies of Pernambuco, with some notes on their taxonomy, ecology, distribution and epidemiological relevanc

Analysis of the genetic structure of allopatric populations of Lutzomyia umbratilis using the period clock gene

Submitted by Adagilson Silva ([email protected]) on 2017-09-13T13:47:34Z No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-13T17:23:45Z (GMT) No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5)Made available in DSpace on 2017-09-13T17:23:45Z (GMT). No. of bitstreams: 1 26655040 2015 fre-ana.oa.pdf: 1632788 bytes, checksum: 112764016653875cf14c2bbb5487d1aa (MD5) Previous issue date: 2016-02Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Instituto Leônidas e Maria Deane. Laboratory of Infectious Disease Ecology in the Amazon. Manaus, AM, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Research Center Aggeu Magalhaes. Departament of Microbiology. Recife, PE, Brazil.Federal University of Pernambuco. Departament of Genetic. Recife, PE, Brazil.Oswaldo Cruz Foundation. Instituto Leônidas e Maria Deane. Laboratory of Infectious Disease Ecology in the Amazon. Manaus, AM, Brazil.In South America, Lutzomyia umbratilis is the main vector of Leishmania guyanensis, one of the species involved in the transmission of American tegumentary leishmaniasis. In Brazil, L. umbratilis has been recorded in the Amazon region, and an isolated population has been identified in the state of Pernambuco, Northeastern region. This study assessed the phylogeographic structure of three allopatric Brazilian populations of L. umbratilis. Samples of L. umbratilis were collected from Rio Preto da Eva (north of the Amazon River, Amazonas), from Manacapuru (south of the Amazon River), and from the isolated population in Recife, Pernambuco state. These samples were processed to obtain sequences of the period gene. Phylogenetic analysis revealed the presence of two distinct monophyletic clades: one clade comprised of the Recife and Rio Preto da Eva samples, and one clade comprised of the Manacapuru samples. Comparing the Manacapuru population with the Recife and Rio Preto da Eva populations revealed high indices of interpopulational divergence. Phylogenetic analysis indicated that geographical distance and environmental differences have not modified the ancestral relationship shared by the Recife and Rio Preto da Eva populations. Genetic similarities suggest that, in evolutionary terms, these populations are more closely related to each other than to the Manacapuru population. These results confirm the existence of an L. umbratilis species complex composed of at least two incipient species

MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL

In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra- and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil

Phenotypic and genotypic variations among three allopatric populations of Lutzomyia umbratilis, main vector of Leishmania guyanensis

Submitted by Adagilson Silva ([email protected]) on 2017-09-20T14:04:18Z No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5)Approved for entry into archive by Adagilson Silva ([email protected]) on 2017-09-20T14:12:14Z (GMT) No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5)Made available in DSpace on 2017-09-20T14:12:14Z (GMT). No. of bitstreams: 1 26338469 2015 fre-phe.oa.pdf: 2163638 bytes, checksum: d8623ba27d611eec48f95113299aba1e (MD5) Previous issue date: 2015-09-04Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, BrasilIn South America, Lutzomyia umbratilis is the main vector of Leishmania guyanensis, one of the species involved in the transmission of American tegumentary leishmaniasis. In Brazil, L. umbratilis has been recorded in the Amazon region, and in the state of Pernambuco, Northeastern region, where an isolated population has been identified. This study assessed the phylogeographic structure and size and shape differences of the wing of three Brazilian populations

Allele Frequencies and Forensic Data of 25 STR Markers for Individuals in Northeast Brazil

Identifying DNA markers such as Short Tandem Repeats (STR) can be used to investigate genetic diversity based on levels of heterozygosity within and between populations. Allele frequencies and forensic data for STRs were obtained from a sample of 384 unrelated individuals living in Bahia, Northeastern Brazil. Thus, the present study aimed to identify the allele frequency distribution, in addition to the forensic and genetic data, of 25 STR loci in the population of Bahia. Buccal swabs or fingertip punctures were utilized to amplify and detect 25 DNA markers. The most polymorphic loci were SE33 (43), D21S11, and FGA (21). The least polymorphic were TH01 (6), TPOX, and D3S1358 (7). Forensic and statistical data were obtained through data analysis, which revealed a large genetic diversity, with an average value of 0.813 for the analyzed population. The present study was more robust than previous STR marker studies and will contribute to future research on population genetics in Brazil and worldwide. The results of this study allowed the establishment of haplotypes found in the forensic samples of Bahia State to serve as a reference in the elucidation of criminal cases and paternity tests, as well as population and evolutionary investigations