Motivations, managerial skills and economic performance in small business enterprises

SIGLEAvailable from British Library Document Supply Centre- DSC:D33291/80 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Extraction and purification of hyaluronoglucosidase (EC 3.2.1.35) from Norway lobster (Nephrops norvegicus)

The waste of Norway lobster (Nephrops norvegicus also known as Dublin Bay prawn and scampi) constitutes 75-80 of the whole animal by weight. This includes the hepatopancreas which is a good source of hyaluronoglucosidase (EC 3.2.1.35), commonly referred to as hyaluronidase. This enzyme was partially purified by acetone fractionation, ion-exchange column chromatography on a Type-I polystyrene-based anion-exchange resin, Amberlite(TM) IRA 420 and subsequently by gel filtration on Sephadex(TM) G-200. The anion-exchange step of the purification was optimised by including a wash with 10 mM sodium acetate buffer containing 12.5 mM NaCl after loading the column with the enzyme preparation. This was followed by gradient elution with 12.5-500 mM NaCl in 500 ml of the same buffer. The gel filtration step was optimised using Sephacryl(TM) S-200-HR gel filtration medium. As a result of these modifications to the purification process a 763-fold purification was achieved, with 32 of the enzyme from the original crude extract in 0.25 M sucrose solution being recovered. A loss of 41 of the enzyme activity was recorded during acetone fractionation and 34 during gel filtration. However, recovery from the anion-exchange step using Amberlite(TM) IRA 420 was as high as 81. A sample of the purified extract was subjected to native polyacrylamide gel electrophoresis with PhastGel(TM) Gradient 10-15 using PhastGel Native Buffer Strips which indicated the presence of three proteins. Copyright (C) 1999 Elsevier Science Ltd

Yours Faithfully Edna Welthorpe (Mrs) [the event]

Co-creator and co-curator of Yours Faithfully Edna Welthorpe (Mrs) with Dr Emma Parker of Leicester University. Yours Faithfully is a tribute project to the 60's author and playwright Joe Orton. To mark the 50th anniversary of the passing of Joe Orton. In collaboration with the Orton Archive in Leicester University we created a multi disciplined project. THE EVENT On 9 August 2017, actors, writers and comedians gathered at The Little Theatre, Leicester, to celebrate the life, work and legacy of Joe Orton 50 years after his death. The venue for the event has special significance since, in 1949, Joe (then John) Orton performed in Shakespeare’s Richard III at The Little Theatre as a member of Leicester Drama Society. Actor Frances Barber, who played Leonie Orton in the film Prick Up Your Ears (1987), read the original Edna Welthorpe letters; Chris Shepherd screened his new animation inspired by Orton’s work; authors read their new Edna letters; Leonie Orton shared memories of her brother’s pranks in Leicester; David Quantick and Graham Fellows (John Shuttleworth) discussed Orton’s impact on contemporary comedy. Winner of the Wildcard Award at the Saboteur Awards 201