Imaging Formation Algorithm of the Ground and Space-Borne Hybrid BiSAR Based on Parameters Estimation from Direct Signal

This paper proposes a novel image formation algorithm for the bistatic synthetic aperture radar (BiSAR) with the configuration of a noncooperative transmitter and a stationary receiver in which the traditional imaging algorithm failed because the necessary imaging parameters cannot be estimated from the limited information from the noncooperative data provider. In the new algorithm, the essential parameters for imaging, such as squint angle, Doppler centroid, and Doppler chirp-rate, will be estimated by full exploration of the recorded direct signal (direct signal is the echo from satellite to stationary receiver directly) from the transmitter. The Doppler chirp-rate is retrieved by modeling the peak phase of direct signal as a quadratic polynomial. The Doppler centroid frequency and the squint angle can be derived from the image contrast optimization. Then the range focusing, the range cell migration correction (RCMC), and the azimuth focusing are implemented by secondary range compression (SRC) and the range cell migration, respectively. At last, the proposed algorithm is validated by imaging of the BiSAR experiment configured with china YAOGAN 10 SAR as the transmitter and the receiver platform located on a building at a height of 109 m in Jiangsu province. The experiment image with geometric correction shows good accordance with local Google images

Genome-Wide Analysis of ATP-Binding Cassette (ABC) Transporters in the Sweetpotato Whitefly, \u3cem\u3eBemisia tabaci\u3c/em\u3e

Background: ABC transporter superfamily is one of the largest and ubiquitous groups of proteins. Because of their role in detoxification, insect ABC transporters have gained more attention in recent years. In this study, we annotated ABC transporters from a newly sequenced sweetpotato whitefly genome. Bemisia tabaci Q biotype is an emerging global invasive species that has caused extensive damages to field crops as well as ornamental plants. Results: A total of 55 ABC transporters containing all eight described subfamilies (A to H) were identified in the B. tabaci Q genome, including 8 ABCAs, 3 ABCBs, 6 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 23 ABCGs and 9 ABCHs. In comparison to other species, subfamilies G and H in both phloem- and blood-sucking arthropods are expanded. The temporal expression profiles of these 55 ABC transporters throughout B. tabaci developmental stages and their responses to imidacloprid, a neonicotinoid insecticide, were investigated using RNA-seq analysis. Furthermore, the mRNA expression of 24 ABC transporters (44% of the total) representing all eight subfamilies was confirmed by the quantitative real-time PCR (RT-qPCR). Furthermore, mRNA expression levels estimated by RT-qPCR and RNA-seq analyses were significantly correlated (r = 0.684, p \u3c 0.01). Conclusions: It is the first genome-wide analysis of the entire repertoire of ABC transporters in B. tabaci. The identification of these ABC transporters, their temporal expression profiles during B. tabaci development, and their response to a neonicotinoid insecticide lay the foundation for functional genomic understanding of their contribution to the invasiveness of B. tabaci

Long Non-Coding RNA XLOC_006753 Promotes the Development of Multidrug Resistance in Gastric Cancer Cells Through the PI3K/AKT/mTOR Signaling Pathway

Background/Aims: The development of multidrug resistance (MDR), which results in disease recurrence and metastasis, is a crucial obstacle to successful chemotherapy for patients with gastric cancer (GC). Long non-coding RNAs (lncRNAs) have been found to play various roles in cancer. This study aimed to investigate the effect of XLOC_006753 on the development of MDR in GC cells. Methods: The expression levels of XLOC_006753 in GC patients and MDR GC cell lines (SGC-7901/5-FU and SGC-7901/DDP cell line) were assessed by qRT-PCR. Statistical analyses were conducted to determine the relationship between XLOC_006753 expression and clinical features and to assess the prognostic value of XLOC_006753 for overall survival and progression-free survival. Then, a CCK-8 assay was used to detect cell proliferation ability and chemosensitivity. Flow cytometry was used to detect cell cycle and cell apoptosis. A wound-healing assay and transwell assay were used to detect cell migration. The expression of markers for MDR, G1/S transition, epithelial–mesenchymal transition (EMT) and PI3K/ AKT/mTOR signaling pathway were examined by western blot. Results: XLOC_006753 was highly expressed in GC patients and MDR GC cell lines (SGC-7901/5-FU and SGC-7901/DDP cell lines), and its high expression was positively associated with metastasis, TNM stage, tumor size, and poor survival in GC patients. Moreover, XLOC_006753 was an independent prognostic biomarker of overall survival and progression-free survival for gastric cancer patients. Knocking down XLOC_006753 in the two MDR GC cell lines significantly inhibited cell proliferation, cell viability, cell cycle G1/S transition, and migration. XLOC_006753 knockdown also promoted apoptosis. Furthermore, western blots showed that XLOC_006753 knockdown decreased some markers of MDR, G1/S transition, and EMT expression, while increasing caspase9 expression and inhibiting the PI3K/AKT/mTOR signaling pathway in SGC-7901/5-FU and SGC-7901/DDP cells. Conclusion: High expression of XLOC_006753 promoted the development of MDR, which was activated by the PI3K/AKT/mTOR pathway in GC cells

Genome Sequencing of the Sweetpotato Whitefly \u3cem\u3eBemisia tabaci\u3c/em\u3e MED/Q

The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future \u27pan-genomic\u27 comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management

Carboxylate-Assisted Carboxylation of Thiophene with CO₂ in the Solvent-Free Carbonate Medium

Direct carboxylation of thiophene with CO2 has been achieved under a relatively mild solvent-free carbonate and carboxylate medium. This base-mediated medium can cleave the very weakly acidic C–H bond without using other limiting reagents, which is one indispensable step in the carboxylation reaction. Product yield varies with different carboxylate salts, and cesium pivalate is the most suitable base additive among targeted simple carboxylate salts. Furthermore, the detailed mechanism of this carboxylation reaction is studied, which involves initial proton abstraction, rendered by carbonate and C–C bond formation, by inserting CO2. The activation energy barrier of the C–H activation step is higher than the following CO2 insertion step, whether for the formation of the mono- and/or di-carboxylate, which indicates that the C–H deprotonation induced by the base is slow and the resulting carbon-centered nucleophile reacts rapidly with CO2

Carboxylate-Assisted Carboxylation of Thiophene with CO2 in the Solvent-Free Carbonate Medium

Direct carboxylation of thiophene with CO2 has been achieved under a relatively mild solvent-free carbonate and carboxylate medium. This base-mediated medium can cleave the very weakly acidic C–H bond without using other limiting reagents, which is one indispensable step in the carboxylation reaction. Product yield varies with different carboxylate salts, and cesium pivalate is the most suitable base additive among targeted simple carboxylate salts. Furthermore, the detailed mechanism of this carboxylation reaction is studied, which involves initial proton abstraction, rendered by carbonate and C–C bond formation, by inserting CO2. The activation energy barrier of the C–H activation step is higher than the following CO2 insertion step, whether for the formation of the mono- and/or di-carboxylate, which indicates that the C–H deprotonation induced by the base is slow and the resulting carbon-centered nucleophile reacts rapidly with CO2

An empirical research on economic growth from industrial structure optimisation in the Three Gorges Reservoir area

Using the panel data of industrial structure and economic growth (EG) in eight key districts and counties in the Three Gorges Reservoir area from 2011 to 2019, EG was used as the explained variable, and industrial structure optimisation was used as the explanatory variable; technological innovation (TI), FTL, FCI and budgetary expenditure (BE) were used as the control variables. Empirical research on whether the industrial structure can have an impact on the EG of the reservoir area was carried out. The research results showed that optimisation of the industrial structure in the reservoir area plays an important role in promoting EG, and the investment of fixed capital and BE has a significant positive impact on the EG of the reservoir area, whereas the level of TI and foreign trade have no important role in promoting EG

Mechanistic Insights into Palladium(II)-Catalyzed Carboxylation of Thiophene and Carbon Dioxide

The mechanism in palladium-catalyzed carboxylation of thiophene and CO2 is investigated using the density functional theory (DFT) calculations, including three consecutive steps of the formation of carbanion through breaking the C–H bond(s) via the palladium acetate, the elimination of acetic acid and the nucleophile attacking the weak electrophile CO2 to form C–C bond. Results show that the C–C bond is formed through taking the three-membered cyclic conformation arrangement involving the interaction of the transition metal and the CO2, and the CO2 insertion step is the rate-determining step for this entire reaction process. Aiming to precisely disclose what factor determine the origin of the activation energy barrier in this carboxylation reaction, the distortion/interaction analysis is performed along with the entire reaction coordinate

Comparison of the Measurement of Long-Term Care Costs between China and Other Countries: A Systematic Review of the Last Decade

Background: The rapid aging of populations in some countries has led to a growing number of the disabled elderly, creating a huge need for Long-Term Care (LTC) and meeting its costs, which is a heavy economic burden on the families of the disabled elderly and governments. Therefore, the measurement of Long-Term Care (LTC) costs has become an important basis for the government to formulate Long-Term Care (LTC) policies, and academic research on Long-Term Care (LTC) costs is also in the process of continuous development and deepening. Methods: This is a systematic review that aims to examine the evidence published in the last decade (2010–2019) regarding the comparison of the measurement of Long-Term Care (LTC) costs between China and other countries. Results: Eighteen Chinese studies and 17 other countries’ studies were included in this review. Most Chinese scholars estimated long-term care costs based on the degree of disability among the disabled elderly. However, the studies of European and American countries are more and more in-depth and comprehensive, and more detailed regarding the post-care cost of specific diseases, such as Parkinson’s disease, Alzheimer’s disease, and epilepsy. Conclusion: In future academic research, we should fully consider the human value of long-term care providers and further study the differences in the long-term care costs of different chronic diseases. In China’s future policymaking, according to the experience of Germany, Sweden, and other countries, it may be an effective way to develop private long-term care insurance and realize the effective complementarity between private long-term care insurance and public long-term care insurance (LTCI)