26 research outputs found
Concept for a Future Super Proton-Proton Collider
Following the discovery of the Higgs boson at LHC, new large colliders are
being studied by the international high-energy community to explore Higgs
physics in detail and new physics beyond the Standard Model. In China, a
two-stage circular collider project CEPC-SPPC is proposed, with the first stage
CEPC (Circular Electron Positron Collier, a so-called Higgs factory) focused on
Higgs physics, and the second stage SPPC (Super Proton-Proton Collider) focused
on new physics beyond the Standard Model. This paper discusses this second
stage.Comment: 34 pages, 8 figures, 5 table
STVNa Attenuates Isoproterenol-Induced Cardiac Hypertrophy Response through the HDAC4 and Prdx2/ROS/Trx1 Pathways
Recent data show that cardiac hypertrophy contributes substantially to the overall heart failure burden. Mitochondrial dysfunction is a common feature of cardiac hypertrophy. Recent studies have reported that isosteviol inhibits myocardial ischemia-reperfusion injury in guinea pigs and H9c2 cells. This work investigated the protective mechanisms of isosteviol sodium (STVNa) against isoproterenol (Iso)-induced cardiac hypertrophy. We found that STVNa significantly inhibited H9c2 cell and rat primary cardiomyocyte cell surface, restored mitochondrial membrane potential (MMP) and morphological integrity, and decreased the expression of mitochondrial function-related proteins Fis1 and Drp1. Furthermore, STVNa decreased reactive oxygen species (ROS) levels and upregulated the expression of antioxidant factors, Thioredoxin 1 (Trx1) and Peroxiredoxin 2 (Prdx2). Moreover, STVNa restored the activity of histone deacetylase 4 (HDAC4) in the nucleus. Together, our data show that STVNa confers protection against Iso-induced myocardial hypertrophy primarily through the Prdx2/ROS/Trx1 signaling pathway. Thus, STVNA is a potentially effective treatment for cardiac hypertrophy in humans
Magnetism-Resolved Separation and Fluorescence Quantification for Near-Simultaneous Detection of Multiple Pathogens
In the modern era
of molecular evidence-based medicine and advanced
biomedical technologies, the rapid, sensitive and specific assay of
multiple pathogens is critical to, but largely absent from, clinical
practice. Therefore, to improve the current ordinary separation and
collection method, we report herein a strategy of magnetism-resolved
separation and fluorescence quantification for near-simultaneous detection
of multiple pathogens, followed by the direct antimicrobial susceptibility
testing (AST). To accomplish this strategy, we utilized aptamer-modified
fluorescent-magnetic multifunctional nanoprobes (apt-FMNPs). FMNPs
with intriguing different magnetic responses and excellent fluorescence
quality were first self-assembled based on metal coordination interaction
using (3-mercaptopropyl) trimethoxysilane, magnetic γ-Fe<sub>2</sub>O<sub>3</sub>, and fluorescent quantum dots as matrix components.
Then, aptamers, which specific to target pathogens of <i>Escherichia
coli O157:H7</i> (<i>E. coli</i>) and <i>Salmonella
typhimurium</i> (<i>S. typ</i>), were conjugated with
FMNPs to yield apt-FMNPs nanoprobes for multiple pathogens assay.
Based on the discrepant magnetic response of pathogen@nanoprobes complex
under the identical external magnetic field, the model bacteria were
fished out by magnetic adsorption at different time points and subjected
to fluorescence quantification with good linear ranges and detection
limits within 1h. Multiple pathogens spiked in real samples were also
effectively detected by the apt-FMNPs and sequentially fished out
for AST assay, which showed similar results to that for pure pathogens.
The apt-FMNPs-based strategy of near-simultaneous detection of multiple
pathogens shows promise for the potential application in the diagnosis
and treatment of pathogen-related infectious diseases