Understanding Members’ Active Participation in a DAO: An Empirical Study on Steemit

One of Blockchain’s great potential is to enable the decentralized autonomous organizations (DAOs), whose organizational structure and operational mechanism fundamentally distinct from traditional organizations. This revolutionary nature of DAOs will definitely influence peoples’ behavior. Steemit is an online community like Reddit, but it’s a DAO enabled by STEEM blockchain. This nature makes Steemit fans both users and owners. As users, people can enjoy social capital from using Steemit, just like using any other social media. As owners, people can get some economic incentives by earning and holding Steem Power, and Steem Power can be seen a kind of share capital. This paper attempts to explore the participation behavior of people in the Steemit community from two perspectives—social capital and share capital. Social feedback and economic feedback received by members also have an impact on participation behavior

Understanding the Determinants of Review Helpfulness in Online Review Sites: An Empirical Study

Online review websites play an important role in customer’s purchase decision-making process for the useful product knowledge contained in the customer-generated reviews. However, the increasing information volume also makes it difficult for customers to identify and consider those attributes relevant to their decision. Based on Information Processing Theory (IPT) and Multiple Pathway Anchoring and Adjustment Model (MPAAM), we proposed three characteristics of online reviews affecting review helpfulness (e.g., attractiveness, representational sufficiency and functional sufficiency) and examined the moderating influences of information volume on these relationships. A large-scale review dataset from Yelp.com are collected and text analysis technique are applied to validate our research model. Our work, which illustrates the disturbance effect of information volume, has implications for both online word-of-mouth and information processing research

Cause analysis of the extreme hourly precipitation and its relationship with the urban heat island intensity in Shenyang, China

Based on the hourly temperature and precipitation data from China national meteorological stations and regional automatic weather stations in Shenyang, the relationship between extreme hourly precipitation (ExHP) and urban heat island Intensity (UHII) is analyzed. Results show that the UHII is higher at night and in the early morning. The ExHP events mostly occur at night in summer when the UHII is relatively high. The spatial distribution of UHII in Shenyang is consistent with the economic development and the transportation density. Denser population and transportation, and high-rise buildings in the urban center contribute to higher UHII. There are three types of ExHP, namely the abrupt-type ExHP, the growing-type ExHP and the continuous-type ExHP. The overall variation characteristics of the three types of ExHP are relatively consistent. Their UHII values are positive and relatively stable in 6–12 h before the start of ExHP. The UHII begins to increase dramatically about 6 h before the ExHP, but decreases obviously and turn negative after the precipitation begins. Before the abrupt ExHP, the UHII is relatively high and can rapidly return to positive after the ending of ExHP. The UHII of the abrupt-type ExHP is remarkably larger than that of the growing-type and continuous-type ExHP. The UHII before and after the abrupt-type ExHP differs greatly. Before the abrupt ExHP, the UHII is high in the center and low at both ends, and the high-value areas of UHII are mainly located in the urban area. After the abrupt-type EXHP, the UHII drops and turn negative in the whole area. The UHII is obviously increasing with urbanization. The diurnal variation of UHII is enormous, which is higher at night than during the daytime. The increasing UHII can cause abnormal air pressure in cities and villages. The air in the lower atmospheric layer of the city can be heated and expanded, hence resulting in lower local air pressure. Then, the lower air pressure can promote the convergence and upward movement of air, hence facilitating the establishment of UHII circulation. This phenomenon is particularly distinct at night, which is conducive to the occurrence of ExHP events

Transcriptomic analysis reveals ethylene signal transduction genes involved in pistil development of pumpkin

Development of female flowers is an important process that directly affects the yield of Cucubits. Little information is available on the sex determination and development of female flowers in pumpkin, a typical monoecious plant. In the present study, we used aborted and normal pistils of pumpkin for RNA-Seq analysis and determined the differentially expressed genes (DEGs) to gain insights into the molecular mechanism underlying pistil development in pumpkin. A total of 3,817 DEGs were identified, among which 1,341 were upregulated and 2,476 were downregulated. The results of transcriptome analysis were confirmed by real-time quantitative RT-PCR. KEGG enrichment analysis showed that the DEGs were significantly enriched in plant hormone signal transduction and phenylpropanoid biosynthesis pathway. Eighty-four DEGs were enriched in the plant hormone signal transduction pathway, which accounted for 12.54% of the significant DEGs, and most of them were annotated as predicted ethylene responsive or insensitive transcription factor genes. Furthermore, the expression levels of four ethylene signal transduction genes in different flower structures (female calyx, pistil, male calyx, stamen, leaf, and ovary) were investigated. The ethyleneresponsive DNA binding factor, ERDBF3, and ethylene responsive transcription factor, ERTF10, showed the highest expression in pistils and the lowest expression in stamens, and their expression levels were 78- and 162-times more than that in stamens, respectively. These results suggest that plant hormone signal transduction genes, especially ethylene signal transduction genes, play an important role in the development of pistils in pumpkin. Our study provides a theoretical basis for further understanding of the mechanism of regulation of ethylene signal transduction genes in pistil development and sex determination in pumpkin

Regulation of Free Fatty Acid Receptor 4 on Inflammatory Gene Induced by LPS in Large Yellow Croaker (Larimichthys crocea)

Free fatty acid receptor 4 (FFAR4) plays a key role in regulating the inflammatory response in mammals. The present study aimed to investigate the function of large yellow croaker FFAR4 on inflammation. In the present study, ffar4 was widely expressed in 10 tissues of large yellow croaker including gill, head kidney and spleen. Further studies showed that treatment of head kidney macrophages with agonists (TUG891 or GSK137647A) or overexpression of ffar4 reduced the mRNA expression of pro-inflammatory genes induced by LPS, and increased the expression of pparγ. Treatment of macrophages with antagonist AH7614 increased the mRNA expression of pro-inflammatory genes induced by LPS, and decreased the mRNA expression of pparγ. In order to verify the immunomodulatory effect of PPARγ, PPARγ was overexpressed in macrophages which significantly reduced the mRNA expression of pro-inflammatory genes il6, il1β, il8, tnfα and cox2. Moreover, results of dual-luciferase assays showed that PPARγ downregulated the transcriptional activity of il6 and il1β promoters. In conclusion, FFAR4 showed anti-inflammatory effects on LPS-induced inflammation in large yellow croaker

Dynamic Responses of Continuous Girder Bridges with Uniform Cross-Section under Moving Vehicular Loads

To address the drawback of traditional method of investigating dynamic responses of the continuous girder bridge with uniform cross-section under moving vehicular loads, the orthogonal experimental design method is proposed in this paper. Firstly, some empirical formulas of natural frequencies are obtained by theoretical derivation and numerical simulation. The effects of different parameters on dynamic responses of the vehicle-bridge coupled vibration system are discussed using our own program. Finally, the orthogonal experimental design method is proposed for the dynamic responses analysis. The results show that the effects of factors on dynamic responses are dependent on both the selected position and the type of the responses. In addition, the interaction effects between different factors cannot be ignored. To efficiently reduce experimental runs, the conventional orthogonal design is divided into two phases. It has been proved that the proposed method of the orthogonal experimental design greatly reduces calculation cost, and it is efficient and rational enough to study multifactor problems. Furthermore, it provides a good way to obtain more rational empirical formulas of the DLA and other dynamic responses, which may be adopted in the codes of design and evaluation

Toll‐like receptor‐mediated IRE1α activation as a therapeutic target for inflammatory arthritis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/1/embj2013183-sup-0004-SourceData-S4.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/2/embj2013183-sup-0001.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/3/embj2013183-sup-0008-SourceData-S8.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/4/embj2013183-sup-0005-SourceData-S5.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/5/embj2013183-sup-0001-SourceData-S1.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/6/embj2013183-sup-0009-SourceData-S9.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/7/embj2013183-sup-0006-SourceData-S6.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/8/embj2013183-sup-0002-SourceData-S2.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/9/embj2013183-sup-0010-SourceData-S10.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/10/embj2013183-sup-0007-SourceData-S7.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/11/embj2013183-sup-0003-SourceData-S3.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/12/embj2013183.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/13/embj2013183.reviewer_comments.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/102185/14/embj2013183-sup-0011-SourceData-S11.pd

CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10

Brain metastasis (br-met) develops in an immunologically unique br-met niche. Central nervous system-native myeloid cells (CNS-myeloids) and bone-marrow-derived myeloid cells (BMDMs) cooperatively regulate brain immunity. The phenotypic heterogeneity and specific roles of these myeloid subsets in shaping the br-met niche to regulate br-met outgrowth have not been fully revealed. Applying multimodal single-cell analyses, we elucidated a heterogeneous but spatially defined CNS-myeloid response during br-met outgrowth. We found Ccr2+ BMDMs minimally influenced br-met while CNS-myeloid promoted br-met outgrowth. Additionally, br-met-associated CNS-myeloid exhibited downregulation of Cx3cr1. Cx3cr1 knockout in CNS-myeloid increased br-met incidence, leading to an enriched interferon response signature and Cxcl10 upregulation. Significantly, neutralization of Cxcl10 reduced br-met, while rCxcl10 increased br-met and recruited VISTAHi PD-L1+ CNS-myeloid to br-met lesions. Inhibiting VISTA- and PD-L1-signaling relieved immune suppression and reduced br-met burden. Our results demonstrate that loss of Cx3cr1 in CNS-myeloid triggers a Cxcl10-mediated vicious cycle, cultivating a br-met-promoting, immune-suppressive niche

NOTCH1 Signaling Promotes Human T-Cell Acute Lymphoblastic Leukemia Initiating Cell Regeneration in Supportive Niches

Leukemia initiating cells (LIC) contribute to therapeutic resistance through acquisition of mutations in signaling pathways, such as NOTCH1, that promote self-renewal and survival within supportive niches. Activating mutations in NOTCH1 occur commonly in T cell acute lymphoblastic leukemia (T-ALL) and have been implicated in therapeutic resistance. However, the cell type and context specific consequences of NOTCH1 activation, its role in human LIC regeneration, and sensitivity to NOTCH1 inhibition in hematopoietic microenvironments had not been elucidated.We established humanized bioluminescent T-ALL LIC mouse models transplanted with pediatric T-ALL samples that were sequenced for NOTCH1 and other common T-ALL mutations. In this study, CD34(+) cells from NOTCH1(Mutated) T-ALL samples had higher leukemic engraftment and serial transplantation capacity than NOTCH1(Wild-type) CD34(+) cells in hematopoietic niches, suggesting that self-renewing LIC were enriched within the NOTCH1(Mutated) CD34(+) fraction. Humanized NOTCH1 monoclonal antibody treatment reduced LIC survival and self-renewal in NOTCH1(Mutated) T-ALL LIC-engrafted mice and resulted in depletion of CD34(+)CD2(+)CD7(+) cells that harbor serial transplantation capacity.These results reveal a functional hierarchy within the LIC population based on NOTCH1 activation, which renders LIC susceptible to targeted NOTCH1 inhibition and highlights the utility of NOTCH1 antibody targeting as a key component of malignant stem cell eradication strategies