The Effect of Organic-cr Dietary Supplementation on Stress Response in Transport-stressed Beef Cattle

Transportation over long distances resulted in stress at animal. Under these circumstances, animalusually manifest depression and the impact on physiological condition changes and loss of body weight.The objectives of the research were to examine effect supplementation of organic-Cr type into diets intransport-stress beef cattle on physiological condition, haematochemical (included were haematologicalcondition and blood chemical) and body weight changes. The experiment was conducted using 16 beefcattle those were transported by truck for a distance of 400 km from Malangbong to Tangerang. Theexperiment was arranged by Completely Randomized Design with four treatments and four replications.The dietary treatments consisted of R0 (basal diet without Cr supplemented), R1 (R0+3ppm organic-Crresulted of alkali hydrolysis), R2 (R0+3 ppm organic-Cr resulted from bioprocess), R3 (R0+3 ppmorganic-Cr resulted from bioremediation). The result indicated that type of organic-Cr supplementationat 3 ppm in diet did not influence physiological condition, haematochemical and body weight at beefcattle transported for seven hours. There was indication that beef cattle fed on control diet (without Cr)showed a stress symptom, their loss of body weight were higher (5.41%) compared to beef given dietcontains organic-Cr (3.72%, 5.04% and 4.83%, respectively for R1, R2 and R3)

Enantioselective Desymmetrization of Bicyclic Hydrazines using a <i>C</i>₂‑Symmetric N‑Heterocyclic Carbene (NHC) Palladium Complex as Catalyst

The first example of palladium-catalyzed enantioselective desymmetrization of 2,3-bicyclic hydrazines with arylboronic acids through a ring-opening process is described by using a chiral <i>C</i>₂-symmetric N-heterocyclic carbene (NHC) palladium complex as the catalyst. The reaction can be performed under convenient conditions to give <i>trans</i>-1,2-disubstituted 3-cyclopentenes <b>3</b> with high regioselectivity in good to excellent yields (up to 95%) and moderate to good enantioselectivities (up to 88% ee)

Enantioselective Synthesis of Polycyclic Indole Derivatives Based on aza-Morita–Baylis–Hillman Reaction

A chiral phosphine-catalyzed asymmetric aza-Morita–Baylis–Hillman reaction between indole-derived sulfonyl imines and bis­(3-chlorophenyl)­methyl acrylate has been developed, giving the desired adducts in good yields and enantiomeric excess values along with the further transformations to polycyclic indoles such as dihydropyrido­[1,2-<i>a</i>]­indole and dihydropyrazino­[1,2-<i>a</i>]­indole skeleton

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-2

-ODN group and control group compared with other treatment groups. * < 0.01, one-way ANOVA. A significant interaction was observed between As-ODN-hTR and ATRA treatment. * * F = 4.507, = 0.041, two-way ANOVA.<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-0

Rs in each experimental group is shown at the end of treatment (L = left side, R = right side). Both As-ODN and ATRA treatment resulted in an inhibition of tumor volume and tumor weight as compared to control group. * < 0.01, one-way ANOVA. In addition, highly significant interactions exist between hTR As-ODN and ATRA. * * F = 10.743, = 0.002, two-way ANOVA. The combination of As-ODN and ATRA resulted in a significant enhancement of the reduction in tumor growth when compared with monotherapy of As-ODN or ATRA alone (< 0.01).<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-1

Control group, (B) S group, (C) As group, (D) ATRA group, (E)S/ATRA group and (F) As/ATRA group. Arrows indicate positive cells. Scale bar = 50 μm. The average Integrated Optical Density (IOD) of each group was performed by a computer-assisted quantitation. (G) IOD data is shown as the mean ± SE (n = 10). The average IODs in As-ODN and ATRA treated groups were significantly increased as compared to S-ODN group and control group. * < 0.01, one-way ANOVA. Significant interaction was observed between As-ODN and ATRA treatment. * * F = 45.918, < 0.01, two-way ANOVA. (H) Electron microscopy showed typical feature of apoptotic cell in As-ODN and ATRA treatment groups. Whereas, no ultrastructural changes were observed in S-ODN group and control group (data not shown).<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-3

Asmic staining in (C) As group, (D) ATRA group and (E) S/ATRA group, extremely weak Bcl-2 staining in (F) As/ATRA treated group. (G) Similar moderate Bax cytoplasmic staining of tumor cells in all treatment groups. Representative section of As/ATRA group is shown. Scale bar = 50 μm. Computer-assisted quantitation of immunohistochemical staining was performed. (H) The average IODs of Bcl-2 and Bax were shown as the mean ± SE (n = 10). The average IODs in As-ODN and ATRA treated groups were significantly decreased as compared to S-ODN group and control group. * < 0.01, one-way ANOVA. Significant interaction was observed between As-ODN-hTR and ATRA treatment. * * F = 35.836, < 0.01, two-way ANOVA. There was no significant difference of Bax expression in all treatment groups.<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma-4

Rs in each experimental group is shown at the end of treatment (L = left side, R = right side). Both As-ODN and ATRA treatment resulted in an inhibition of tumor volume and tumor weight as compared to control group. * < 0.01, one-way ANOVA. In addition, highly significant interactions exist between hTR As-ODN and ATRA. * * F = 10.743, = 0.002, two-way ANOVA. The combination of As-ODN and ATRA resulted in a significant enhancement of the reduction in tumor growth when compared with monotherapy of As-ODN or ATRA alone (< 0.01).<p><b>Copyright information:</b></p><p>Taken from "Antisense oligonucleotides and all-trans retinoic acid have a synergistic anti-tumor effect on oral squamous cell carcinoma"</p><p>http://www.biomedcentral.com/1471-2407/8/159</p><p>BMC Cancer 2008;8():159-159.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2427037.</p><p></p

Diastereo- and Enantioselective Construction of Oxindole-Fused Spirotetrahydrofuran Scaffolds through Palladium-Catalyzed Asymmetric [3+2] Cycloaddition of Vinyl Cyclopropanes and Isatins

A novel asymmetric formal [3+2] cycloaddition of vinyl cyclopropanes and isatins in the presence of Pd₂(dba)₃ and the chiral imidazoline-phosphine ligand (a<i>S,R,R</i>)-<b>L3</b> has been developed, affording the corresponding highly functionalized oxindole-fused spirotetrahydrofuran frameworks in good yields along with good diastereo- and high enantioselectivities under mild conditions

Palladium-Catalyzed Asymmetric Formal [3+2] Cycloaddition of Vinyl Cyclopropanes and β,γ-Unsaturated α‑Keto Esters: An Effective Route to Highly Functionalized Cyclopentanes

Palladium-catalyzed asymmetric formal [3+2] cycloaddition of vinyl cyclopropanes and β,γ-unsaturated α-keto esters proceeded smoothly in the presence of chiral imidazoline–phosphine ligands to give the corresponding highly functionalized cyclopentanes in good yields along with high diastereo- and enantioselectivities under mild conditions