Multi-objective sustainability optimization of CCHP systems considering the discreteness of equipment capabilities

The value of waste heat had led to an extensive study on Combined Cooling, Heating and Power (CCHP) system in recent decades, but the following three research gaps still need to be tackled to achieve a better economic and environmental performance. Firstly, the complete discreteness of equipment capabilities had not been considered. It means that multiple units with different capacities cannot be selected for a type of equipment. Then, the ambiguity and subjectivity existing in decision-makers/stakeholders’ judgments on the importance of objectives are usually ignored. Finally, an easily understood and comprehensive environmental indicator based on life cycle perspective for system optimization had not been established. Thus, the aim of this study is to establish a mathematical framework to help the stakeholders select the optimal configurations, capacities, and operation conditions of CCHP system while narrowing the above three research gaps to avoid the sub-optimal solutions. Subsequently, a hypothetical case was used to verify the validity of the proposed model, along with analysis of system performance. The results indicate that the CCHP system is superior to the conventional systems, and the proposed mathematical model in this paper can improve the performance of CCHP system in terms of economy, environment, and energy

Chromosome-level genome assembly of a high-altitude-adapted frog (Rana kukunoris) from the Tibetan plateau provides insight into amphibian genome evolution and adaptation

Background The high-altitude-adapted frog Rana kukunoris, occurring on the Tibetan plateau, is an excellent model to study life history evolution and adaptation to harsh high-altitude environments. However, genomic resources for this species are still underdeveloped constraining attempts to investigate the underpinnings of adaptation. Results The R. kukunoris genome was assembled to a size of 4.83 Gb and the contig N50 was 1.80 Mb. The 6555 contigs were clustered and ordered into 12 pseudo-chromosomes covering similar to 93.07% of the assembled genome. In total, 32,304 genes were functionally annotated. Synteny analysis between the genomes of R. kukunoris and a low latitude species Rana temporaria showed a high degree of chromosome level synteny with one fusion event between chr11 and chr13 forming pseudo-chromosome 11 in R. kukunoris. Characterization of features of the R. kukunoris genome identified that 61.5% consisted of transposable elements and expansions of gene families related to cell nucleus structure and taste sense were identified. Ninety-five single-copy orthologous genes were identified as being under positive selection and had functions associated with the positive regulation of proteins in the catabolic process and negative regulation of developmental growth. These gene family expansions and positively selected genes indicate regions for further interrogation to understand adaptation to high altitude. Conclusions Here, we reported a high-quality chromosome-level genome assembly of a high-altitude amphibian species using a combination of Illumina, PacBio and Hi-C sequencing technologies. This genome assembly provides a valuable resource for subsequent research on R. kukunoris genomics and amphibian genome evolution in general.Peer reviewe

AAV2-Mediated Subretinal Gene Transfer of hIFN-α Attenuates Experimental Autoimmune Uveoretinitis in Mice

BACKGROUND: Recent reports show that gene therapy may provide a long-term, safe and effective intervention for human diseases. In this study, we investigated the effectiveness of adeno-associated virus 2 (AAV2) based human interferon-alpha (hIFN-α) gene therapy in experimental autoimmune uveoretinitis (EAU), a classic model for human uveitis. METHODOLOGY/PRINCIPAL FINDINGS: An AAV2 vector harboring the hIFN-α gene (AAV2.hIFN-α) was subretinally injected into B10RIII mice at two doses (1.5×10(6) vg, 1.5×10(8) vg). AAV2 vector encoding green fluorescent protein (AAV2.GFP) was used as a control (5×10(8) vg). The expression of hIFN-α in homogenized eyes and serum was detected by ELISA three weeks after injection. The biodistribution of vector DNA in the injected eyes, contralateral eyes and distant organs was determined by PCR. EAU was induced by immunization with IRBP(161-180) three weeks following vector injections, and evaluated clinically and pathologically. IRBP-specific proliferation and IL-17 expression of lymphocytes from the spleen and lymph nodes were assayed to test the influence of the subretinal delivery of AAV2.hIFN-α on the systemic immune response. hIFN-α was effectively expressed in the eyes from three weeks to three months following subretinal injection of AAV2.hIFN-α vector. DNA of AAV2.GFP was observed only in the injected eyes, but not in the distant organs or contralateral eyes. Subretinal injection of both doses significantly attenuated EAU activity clinically and histologically. For the lower dose, there was no difference concerning lymphocyte proliferation and IL-17 production among the AAV2.hIFN-α, AAV2.GFP and PBS injected mice. However, the higher dose of AAV2.hIFN-α significantly suppressed lymphocyte proliferation and IL-17 production. CONCLUSIONS/SIGNIFICANCE: Subretinal delivery of AAV2.hIFN-α lead to an effective expression within the eye for at least three months and significantly attenuated EAU activity. AAV2.hIFN-α was shown to inhibit the systemic IRBP-specific immune response

The Effects of Pore Geometry on Late Time Solute Transport with the Presence of Recirculation Zone

The solute transport process in porous media is central to understanding many geophysical processes and determines the success of engineered applications. However, fundamental understanding of solute transport in heterogeneous porous media remains challenging especially when inertial effects are significant. To address this challenge, we employed direct numerical simulations in a variety of intrapore geometries at a high Reynolds number (Re = 10) flow regime, where recirculation zones (RZs) are present with significant inertial effects. We find that the volume of RZs depends on pore geometries. Moreover, RZs serve as an immobile domain that can trap and release solutes that lead to non-Fickian transport, characterized by the early arrival and heavy tailing of breakthrough curves and bimodal residence time distributions (RTDs). Lastly, the late time portion of RTDs is fitted to the power law function with determined exponent n, where n depends on the pore geometries and consequently the volume of RZs. Our study sheds light on the mechanisms of an immobile zone on the solute transport, especially improving our understanding of late time transport tailing in pressurized heterogeneous porous media

A Bayesian displacement field approach to accurate registration of SAR images

Precise registration of synthetic aperture radar (SAR) images is a nontrivial task since a change in radar-acquisition geometry generates image shifts. In existing system, either the transformation functions are oversimplified, or external measures such as digital elevation model and flight track are required to be precise. In this paper, we proposed a generative Bayesian approach to modelling the displacement vectors that map the position of each pixel in the image, thus avoiding degradation of the transformation function. Rather than providing a point estimate for the transformation function, the proposed method yields a full posterior density function of the transformation function. Especially, the Bayesian model learns all the parameters adaptively, and the procedure is fully automatic. The proposed model is comparable in accuracy to state-of-the-art optical flow methods on the challenging Sintel benchmarks, and outperforms currently published SAR image registration methods on some real SAR data with critical scenes

Mechanism of self-recovery of hydrophobicity after surface damage of lotus leaf

Abstract The surfaces of lotus leaves with micro- and nano-waxy cuticle structures are superhydrophobic and possess a self-healing ability to regain hydrophobicity after damage. Inspired by this phenomenon, the problem of water-repellent coatings used in natural environments failing to perform after damage can be solved if these coatings are endowed with rapid self-repair and self-growth functions. However, there has been almost no exploration into the hydrophobicity self-repair process in lotus leaves. The changes in surface morphology during the hydrophobicity recovery process are not understood. There is a lack of research on the hydrophobicity recovery in lotus leaves. In this study, the damage and recovery experiments on lotus leaf surfaces were carried out in an artificial climate chamber, and the water repellency recovery process and typical water repellency roughness parameters regained time were obtained. Upon analyzing the differences in the recovery process of different damage types, the recovery mechanism after lotus leaf surface damage was obtained. Finally, it was found that the microscopic roughness determined the static contact angle (WCA) of the lotus leaf surface, and the nanoscopic roughness determined the rolling angle (SA). The dual factors of the recovery of the extruded epidermal tissue and the regeneration of the epidermal wax crystals determined the hydrophobicity recovery process in damaged lotus leaves