8 research outputs found
Brd4 and HEXIM1: Multiple roles in P-TEFb regulation and cancer
10.1155/2014/232870BioMed Research International2014
Treatment of HMBA differentiates hPSCs into mesoderm-ectoderm lineages.
<p>HES-3 cells were incubated with 1, 3, 5 or 10 mM HMBA for 7 PDs. RNAs prepared from the treated cells were analyzed by QRT-PCR to determine the expression of (A) pluripotent (NANOG and OCT3/4), (B) endodermal (AFP and GATA4), (C) mesodermal (Col2A1, IGF2, and ACTC1), and (D) ectodermal genes (MSX1, PAX6, and SOX1). Results were reproducible, and data from one experiment are presented. Error bars indicate the standard deviations between triplicates. *<i>P</i><0.05 versus control.</p
Treatment of HMBA increases expression of HEXIM1 and induces differentiation in HES-3 cells.
<p>(A) HES-3 cells were incubated with 1, 3, 5, or 10 mM HMBA for 7 PDs, followed by FACS analysis. Percentages of cells expressing pluripotent markers, including OCT3/4, PODXL, and Tra-1-60, were indicated (i.e. open histograms). Cells treated with 1% ethanol were used as the vehicle control. (B) HEXIM1 mRNA levels of the HMBA-treated HES-3 cells were measured by QRT-PCR. (C) HEXIM1 and OCT3/4 protein levels of the HMBA-treated HES-3 cells were examined by western blotting. Actin was used as a loading control.</p
High-level expression of HEXIM1 up-regulates the marker genes of three germ layers in hPSCs.
<p>HES-3 cells were transiently transfected with a HEXIM1 expression plasmid, followed by antibiotic selection for the HEXIM1-transfected cells. The differentiated and undifferentiated populations of the HEXIM1-transfected HES-3 cells were sorted by FACS analysis using an anti-TRA-1-60 antibody. The mRNAs prepared from the TRA-1-60 positive and negative HEXIM1-transfected HES-3 cells (i.e. HEXIM1 Tra pos. and HEXIM1 Tra neg, respectively) were analyzed by QRT-PCR to determine the expression of (A) pluripotent (NANOG and OCT3/4), (B) endodermal (AFP and GATA4), (C) mesodermal (Col2A1, IGF2, and ACTC1), and (D) ectodermal genes (MSX1, PAX6, and SOX1). Results were reproducible, and data from one experiment are presented. Error bars indicate the standard deviations between triplicates. *<i>P</i><0.05 versus control.</p
HEXIM1 Induces Differentiation of Human Pluripotent Stem Cells
10.1371/journal.pone.0072823PLoS ONE88-POLN