Comparative analysis of the <i>in-vitro</i> antioxidant activity and bioactive compounds of flaxseed in China according to variety and geographical origin

<p>The present study aimed to use a chemometric study to compare the <i>in-vitro</i> antioxidant activities and bioactive compounds of flaxseed based on the variety and geographical origins, and further explore the specific components constructed the endogenous antioxidant system of flaxseed in China. The differential contents of α-linolenic acid (35.83–58.91%), lignans (11.37–33.31 mg/g), total phenolic acids (109.93–246.88 mg/100 g) and phytosterols (56.52–125.12 mg/g) of 32 flaxseed varieties were observed. Concomitantly, the <i>in-vitro</i> antioxidant activities varied from 32.56 to 46.22 mg TE/100 g, 0.58 to 1.08 mg TE/g, and 14.22 to 36.14 mmol TE/g as determined by the 2,2’-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing antioxidant power (FRAP) and 2,2′-azinobis-(3-ethylbenzthiazoline 6-sulfonic acid) (ABTS) assays, respectively. Significant correlations were found between the antioxidant activities and contents of free syringic acid, <i>p</i>-coumaric acid glucoside and secoisolariciresinol diglucoside (SDG) (<i>p </i>< 0.01). Principal component analysis showed a relatively dispersed distribution between samples, which was not effective enough to determine the geographical origins of the flaxseed samples. Hierarchical cluster analysis allowed flaxseed samples to be grouped based on their <i>in-vitro</i> antioxidant activities and bioactive component contents, primarily in relation to variety specificity.</p

Flaxseed Oil Alleviates Chronic HFD-Induced Insulin Resistance through Remodeling Lipid Homeostasis in Obese Adipose Tissue

Emerging evidence suggests that higher circulating long-chain n-3 polyunsaturated fatty acids (n-3PUFA) levels were intimately associated with lower prevalence of obesity and insulin resistance. However, the understanding of bioactivity and potential mechanism of α-linolenic acid-rich flaxseed oil (ALA-FO) against insulin resistance was still limited. This study evaluated the effect of FO on high-fat diet (HFD)-induced insulin resistance in C57BL/6J mice focused on adipose tissue lipolysis. Mice after HFD feeding for 16 weeks (60% fat-derived calories) exhibited systemic insulin resistance, which was greatly attenuated by medium dose of FO (M-FO), paralleling with differential accumulation of ALA and its n-3 derivatives across serum lipid fractions. Moreover, M-FO was sufficient to effectively block the metabolic activation of adipose tissue macrophages (ATMs), thereby improving adipose tissue insulin signaling. Importantly, suppression of hypoxia-inducible factors HIF-1α and HIF-2α were involved in FO-mediated modulation of adipose tissue lipolysis, accompanied by specific reconstitution of n-3PUFA within adipose tissue lipid fractions

data of article and supporting information

The files of SEM-PSP, IR and TG, data of performance, XPS and XRD are the data of articles. While the files of supporting information-IR and supporting information-data of recycle performance are the data of supporting informaton

The related XPS spectra of composites. from Novel amphiphilic polyvinylpyrrolidone functionalized silicone particles as carrier for low-cost lipase immobilization

Figure S1. XPS spectra of Si spectra of PVP-SP, PVP-SP@CRL. Table S1. The data of the each peak for the supporting materials. Figure S2. XPS spectra of C 1s spectra of PVP-SP, PVP-SP@CRL. Table S2. The data of the each peak for the supporting materials. Figure S3. XPS spectra of N 1s spectra of PVP-SP, PVP-SP@CRL. Table S3. The data of the each peak for the supporting materials. Fig S4. The FTIR spectra of SP/PVP, SP/Pst-b, SP/Pst-e, SP/Pst-b@CRL, SP/Pst-e@CRL and SP-PVP@CRL. Fig S5. Reusability of the three immobilization lipases