Arbetsplatser i Kortedala och Gårdsten : branschstruktur och lokaliseringsmönster i två bostadsområden i Göteborg /

<p><b>Shoot (A-C) and root (D-F) ion content for <i>HKT1;1</i> native overexpression lines.</b> Statistical significance was determined using Tukey’s HSD test between each line within treatments. Bars with the same letters indicate no significant difference (<i>p</i> < 0.05). Error bars represent standard error of the mean where n = 12–18 plants.</p

Aggregation-Induced Enhancement Effect of Gold Nanoparticles on Triplet Excited State

Remarkable optical properties are posed with gold nanoparticles (AuNPs) due to the excitation of localized surface plasmon resonances, which makes AuNPs affect strongly both the ground state and the excited state of adjacent organic molecules. Compared with the ground state, the effect of AuNPs on excited state of organic molecules is not always fully understood. Here, we performed transient UV–vis absorption experiments to monitor the triplet excited state formation of three cationic dyes and one anionic dye in the presence of two types of gold nanoparticles: the citrate-stabilized AuNPs and ATP-protected AuNPs. It is found that the three cationic dyes can cause efficient aggregation of citrate-stabilized AuNPs, leading to AuNPs aggregates with varied size, whereas the ATP-protected AuNPs can be sustained in the monodispersed state. By comparing the circumstances of aggregated AuNPs and monodispersed AuNPs, we demonstrate that the enhancement effect on triplet excited state formation results from the aggregation of gold nanoparticles and depends on the aggregation size. These findings reveal the aggregation induced plasmon field interaction of AuNPs with excited state population dynamics and may enable new applications of aggregated metal nanoparticles, where aggregates can serve as stronger plasmonic nanoantennas

Analysis of the sensitivity and reproducibility of UNDP-PCR.

<p>(A) Serial dilutions of PCV2 plasmid DNA were detected by conventional PCR. (B) Serial dilutions of PCV2 plasmid DNA were detected by UNDP-PCR. (C) Serial dilutions of PCV2 serum samples were detected by UNDP-PCR. (D) Serial dilutions of PCV2 serum samples were detected by conventional PCR. (E) Indicated three concentration of PCV2 serum samples were detected by UNDP-PCR in triplicates and in three independent runs. Agarose gel electrophoresis data in upper, middle and lower panels are representative three independent experiments. Lane M: Trans 2000 Plus DNA Marker; lane 1–3∶5×10³; lane 4–6∶5×10²; lane 7–9∶5×10¹; lane10: negative samples.</p

Different serotypes and genotypes of porcine circovirus type 2 used in this study.

<p>Different serotypes and genotypes of porcine circovirus type 2 used in this study.</p

Reduced Graphene Oxide Coating with Anticorrosion and Electrochemical Property-Enhancing Effects Applied in Hydrogen Storage System

Low-capacity retention is the most prominent problem of the magnesium nickel alloy (Mg₂Ni), which prevents it from being commercially applied. Here, we propose a practical method for enhancing the cycle stability of the Mg₂Ni alloy. Reduced graphene oxide (rGO) possesses a graphene-based structure, which could provide high-quality barriers that block the hydroxyl in the aqueous electrolyte; it also possesses good hydrophilicity. rGO has been successfully coated on the amorphous-structured Mg₂Ni alloy via electrostatic assembly to form the rGO-encapsulated Mg₂Ni alloy composite (rGO/Mg₂Ni). The experimental results show that ζ potentials of rGO and the modified Mg₂Ni alloy are totally opposite in water, with values of −11.0 and +22.4 mV, respectively. The crumpled structure of rGO sheets and the contents of the carbon element on the surface of the alloy are measured using scanning electron microscopy, transmission electron microscopy, and energy dispersive spectrometry. The Tafel polarization test indicates that the rGO/Mg₂Ni system exhibits a much higher anticorrosion ability against the alkaline solution during charging/discharging. As a result, high-capacity retentions of 94% (557 mAh g^–1) at the 10th cycle and 60% (358 mAh g^–1) at the 50th cycle have been achieved, which are much higher than the results on Mg₂Ni capacity retention combined with the absolute value reported so far to our knowledge. In addition, both the charge-transfer reaction rate and the hydrogen diffusion rate are proven to be boosted with the rGO encapsulation. Overall, this work demonstrates the effective anticorrosion and electrochemical property-enhancing effects of rGO coating and shows its applicability in the Mg-based hydrogen storage system

Analysis of the detection breadth of UNDP-PCR.

<p>(A) Functionalized MMP-p5 were incubated with the DNA derived from different PCV2 representative strains in hybridization buffer at 40°C for 30 min, then oligo4 functionalized Au-NPs were added and incubated at 50°C for 30 min with stirring. After washing and magnetic separation, oligo4 were then detected by PCR as described in Methods. (B) PCV2 poly antibodies functionalized magnetic microparticles incubated with different PCV2 representative strains in assay buffer (1% BSA, 1X PBS, 0.2% Tween20 and 2% sheep serum). Antigen-antibody immune complexes were separated magnetically and were washed twice with 1 ml of magnetic-probe solution that contained PBS pH 7.4, 0.1% BSA and 0.05% Tween20. Then viral DNA were extracted by Viral DNA purification Kit, followed by PCV2-specific PCR detection. Lane: M: Trans 2000 Plus DNA Marker; 1: AF381176; 2: DQ104423; 3: AF112862; 4: EU366323; 5: AY579893; 6: AY391729; 7: FJ644927; 8: AY291317; 9: AY484410; 10: AY181947; 11: KC800634; 12: KC800636; 13: KC800639; 14: KC800644; 15: KC800646; 16: AY193712 (PCV1).</p

Comparison of the detection rate of PCV2 infected preclinical samples by conventional PCR, real-time PCR and UNDP-PCR methods.

<p>Comparison of the detection rate of PCV2 infected preclinical samples by conventional PCR, real-time PCR and UNDP-PCR methods.</p

Agarose gel electrophoresis of the relative viral load levels of 11 positive preclinical specimens identified by UNDP-PCR.

<p>Lane M: Trans 2000 Plus DNA Marker; lane 1–11: preclinical specimens; lane 12∶5×10³ Standards; lane 13∶5×10² Standards; lane 14∶5×10¹ Standards; lane15: negative samples.</p

Analysis of the specificity of UNDP-PCR for detection of PCV2. PRRSV, PRV, PPV, CSFV, PCV1 and the blood DNA of healthy swine were tested by UNDP-PCR as control.

<p>Lane M: DL2000 DNA Marker; lane 1: PCV2; lane 2: the blood DNA of healthy swine; lane 3: PCV1; lane 4: PPV; lane 5: PRV; lane 6: PRRSV; lane 7: CSFV.</p

Comparison of different hybridization probes functionalized magnetic microparticles.

<p>(<b>A</b>) Functionalized magnetic microparticles MMP-p1, -p2, -p3, -p4, -p5, -p6, -p7, -p8 and -p9 were incubated with the DNA derived from different PCV2 representative strains in hybridization buffer at 40°C for 30 min, followed by washing and magnetic separation. MMP-DNA complex were detected by PCV2-specific PCR and PCV1-specific PCR. M: Trans 2000 Plus DNA Marker; 1: AF381176; 2: DQ104423; 3: AF112862; 4: EU366323; 5: AY579893; 6: AY391729; 7: FJ644927; 8: AY291317; 9: AY484410; 10: AY181947; 11: KC800634; 12: KC800636; 13: KC800639; 14: KC800644; 15: KC800646; 16: AY193712 (PCV1). (B) Identification of different hybridization probes functionalized MMPs. The indicated two PCV2 representative strains were incubated with MMP-p1, -p2, -p3, -p4, -p5, -p6, -p7, -p8 and -p9, respectively, followed by PCV2 specific PCR detection. M: Trans 2000 Plus DNA Marker; 1: MMP1; 2: MMP2; 3: MMP3; 4: MMP4; 5: MMP5; 6: MMP6; 7: MMP7; 8: MMP8; 9: MMP9.</p