sj-tif-6-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-tif-6-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

sj-tiff-2-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-tiff-2-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

sj-doc-1-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-doc-1-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

sj-tiff-4-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-tiff-4-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

sj-tiff-5-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-tiff-5-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

sj-tiff-3-evb-10.1177_11769343231212078 – Supplemental material for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma

Supplemental material, sj-tiff-3-evb-10.1177_11769343231212078 for Multi-omics Analysis of Prognostic Significance and Immune Infiltration of FASTK Family Members in Kidney Renal Clear Cell Carcinoma by Guanghui Zhong, Dali Wu, Haiping Chen, Lingfei Yan, Qi Xiang, Yufeng Liu and Tao Wang in Evolutionary Bioinformatics</p

Scavenging Glyoxal and Methylglyoxal by Synephrine Alone or in Combination with Neohesperidin at High Temperatures

α-Dicarbonyl compounds, such as glyoxal (GO) and methylglyoxal (MGO), are a series of chemical hazards that exist in vivo and in vitro, posing a threat to human health. We aimed to explore the scavenging effects on GO/MGO by synephrine (SYN) alone or in combination with neohesperidin (NEO). First, through LC–MS/MS, we confirmed that both SYN and NEO could effectively remove GO and form GO adducts, while NEO could also clear MGO by forming MGO adducts, and its ability to clear MGO was stronger than that of GO. Second, a synergistic inhibitory effect on GO was found when SYN and NEO were used in combination by using the Chou–Talalay method; on the other hand, SYN could promote NEO to clear more MGO, although SYN could not capture MGO. Third, after synthesizing four GO/MGO-adducts (SYN-GO-1, SYN-GO-3, NEO-GO-7, and NEO-MGO-2) and identifying their structure through NMR, strict correlations between the GO/MGO-adducts and the GO/MGO-clearance rate were found when using SYN and NEO alone or in combination. Furthermore, it was inferred that the synergistic effect between SYN and NEO stems from their mutual promotion in capturing more GO by the quantitative analysis of the adducts in the combined model. Finally, a study was conducted on flowers of Citrus aurantium L. var. amara Engl. (FCAVA, an edible tea) rich in SYN and NEO, which could serve as an effective GO and MGO scavenger in the presence of both GO and MGO. Therefore, our study provided well-defined evidence that SYN and NEO, alone or in combination, could efficiently scavenge GO/MGO at high temperatures, whether in the pure form or located in FCAVA

Vision of Death in Ionesco's Bérenger Cycle

Additional file 6: Figure S6. Dox itself has no effect on the expression of miR-690. Real-time qPCR results of miR-690 in Dox-treated C2C12/vectorDox cells. Data are presented as mean ± SD (n = 3)

Groups designed for two-week daily treatment (n = 6/group).

<p>Groups designed for two-week daily treatment (n = 6/group).</p

Apomorphine-induced rotations after two-week daily SP treatment (n = 6/group).

<p>Apomorphine-induced rotations after two-week daily SP treatment (n = 6/group).</p