Indolic Uremic Solutes Enhance Procoagulant Activity of Red Blood Cells through Phosphatidylserine Exposure and Microparticle Release

Increased accumulation of indolic uremic solutes in the blood of uremic patients contributes to the risk of thrombotic events. Red blood cells (RBCs), the most abundant blood cells in circulation, may be a privileged target of these solutes. However, the effect of uremic solutes indoxyl sulfate (IS) and indole-3-acetic acid (IAA) on procoagulant activity (PCA) of erythrocyte is unclear. Here, RBCs from healthy adults were treated with IS and IAA (mean and maximal concentrations reported in uremic patients). Phosphatidylserine (PS) exposure of RBCs and their microparticles (MPs) release were labeled with Alexa Fluor 488-lactadherin and detected by flow cytometer. Cytosolic Ca2+ ([Ca2+]) with Fluo 3/AM was analyzed by flow cytometer. PCA was assessed by clotting time and purified coagulation complex assays. We found that PS exposure, MPs generation, and consequent PCA of RBCs at mean concentrations of IS and IAA enhanced and peaked in maximal uremic concentrations. Moreover, 128 nM lactadherin, a PS inhibitor, inhibited over 90% PCA of RBCs and RMPs. Eryptosis or damage, by indolic uremic solutes was due to, at least partially, the increase of cytosolic [Ca2+]. Our results suggest that RBC eryptosis in uremic solutes IS and IAA plays an important role in thrombus formation through releasing RMPs and exposing PS. Lactadherin acts as an efficient anticoagulant in this process

Hydrochemical Characteristics and Quality Assessment of Groundwater under the Impact of Seawater Intrusion and Anthropogenic Activity in the Coastal Areas of Zhejiang and Fujian Provinces, China

AbstractCoastal groundwater is an important resource in the developed region associated with human health and sustainable economic development. To identify the origins of salinity and evaluate the impact of water-rock interactions, seawater intrusion (SWI), and evaporation on groundwater in the coastal areas of Zhejiang and Fujian provinces, a comprehensive investigation was performed. Meanwhile, nitrate and fluoride indicators resulting from the anthropogenic activity and SWI were also considered. At last, the water quality index (WQI) of coastal groundwater was evaluated with geochemical and multivariate statistical methods. The results indicated that (1) the groundwater in coastal areas of Zhejiang and Fujian provinces has been affected by SWI to varying degrees. The analysis of selected ion ratios (Na+/Cl− and Br−/Cl−) and isotopic compositions showed that SWI is the predominant cause of increasing salinity in the groundwater of Zhejiang Province, while the cause is water-rock interactions (ion exchange and mineral weathering) in Fujian Province. The hydrochemical evolution path of groundwater in Zhejiang Province is Ca/Mg-HCO3 to Na-Cl, while a different pattern of Ca/Mg-HCO3 to Na (Mg/Ca)-Cl occurs in Fujian Province. However, the trend of SWI development in both provinces was freshening. (2) Nitrification, sewage infiltration, and SWI increased the NO3− content in groundwater. Some of the NO3− concentration in Fujian Province exceeds the standard, and the nitrogen pollution was more serious than in Zhejiang Province. The F− content in coastal groundwater was affected by SWI and mineral dissolution; the F− content in Zhejiang Province was higher than in Fujian Province, which was close to the groundwater standard limit. The average WQI value of Zhejiang was 103.61, and the WQI of Fujian was 61.69, indicating that the coastal groundwater quality in Fujian Province was better than in Zhejiang Province. The results of the study revealed the impact of SWI and anthropogenic activity on groundwater in the southern coastal zone of China and will be valuable for sustainable groundwater resource management

Lattice Calculation of the Intrinsic Soft Function and the Collins-Soper Kernel

We calculate the soft function using lattice QCD in the framework of large momentum effective theory incorporating the one-loop perturbative contributions. The soft function is a crucial ingredient in the lattice determination of light cone objects using transverse-momentum-dependent (TMD) factorization. It consists of a rapidity-independent part called intrinsic soft function and a rapidity-dependent part called Collins-Soper kernel. We have adopted appropriate normalization when constructing the pseudo-scalar meson form factor that is needed in the determination of the intrinsic part and applied Fierz rearrangement to suppress the higher-twist effects. In the calculation of CS kernel we consider a CLS ensemble other than the MILC ensemble used in a previous study. We have also compared the applicability of determining the CS kernel using quasi TMDWFs and quasi TMDPDFs. As an example, the determined soft function is used to obtain the physical TMD wave functions (WFs) of pion and unpolarized iso-vector TMD parton distribution functions (PDFs) of proton.Comment: 24 pages, 19 figures, published versio

Nonperturbative determination of the Collins-Soper kernel from quasitransverse-momentum-dependent wave functions

In the framework of large-momentum effective theory at one-loop matching accuracy, we perform a lattice calculation of the Collins-Soper kernel, which governs the rapidity evolution of transversemomentum-dependent (TMD) distributions. We first obtain the quasi-TMD wave functions at three different meson momenta, on a lattice with valence clover quarks on a dynamical highly improved staggered quark sea and lattice spacing a ¼ 0.12 fm from the MILC Collaboration, and renormalize the pertinent linear divergences using Wilson loops. Through one-loop matching to the light-cone wave functions, we determine the Collins-Soper kernel with transverse separation up to 0.6 fm. We study the systematic uncertainties from operator mixing and scale dependence, as well as the impact from higher power corrections. Our results potentially allow for a determination of the soft function and other transverse-momentum-dependent quantities at one-loop accuracy

Pion and Kaon Distribution Amplitudes from Lattice QCD

We present a state-of-the-art lattice QCD calculation of the pion and kaon light-cone distribution amplitudes (DAs) using large-momentum effective theory. The calculation is done at three lattice spacings a≈{0.06,0.09,0.12}  fm and physical pion and kaon masses, with the meson momenta Pz={1.29,1.72,2.15}  GeV. The result is nonperturbatively renormalized in a recently proposed hybrid scheme with self-renormalization, and extrapolated reliably to the continuum as well as the infinite momentum limit. We find a significant deviation of the pion and kaon DAs from the asymptotic form, and a large SU(3) flavor breaking effect in the kaon D

The Impact of the Quality of Digital Economy Development on Manufacturing in the Yangtze River Delta City Cluster

Yangtze River is the most dynamic, open and innovative region in China, as well as an important region for modernization and comprehensive opening. In this paper, under the strategic background of the integration of the Yangtze River Delta region, this paper systematically studies the transformation and upgrading of digitalization and manufacturing from the perspective of urban agglomeration, establishes a measurement model of the digital economy, constructs a comprehensive evaluation system for the quality of digital economy, and intuitively displays and studies the impact mechanism and realization path of digital economy and manufacturing transformation and upgrading. The objective of the study is to examine whether the development of the digital economy is a driver of regional development. The results of the study revealed that the quality of digital economy development in the Yangtze River Delta urban agglomeration has a positive impact on the transformation and upgrading of the local manufacturing industry

Using heat as a tracer to quantify surface water and groundwater interactions in the Baiyangdian wetland

Surface water and groundwater interactions play an important role in hydrological cycle in a wetland ecosystem. Understanding its mechanism and interaction magnitude imposes significant effects in wetland protection and management. In this study, a heat tracer method was taken in the Baiyangdian wetland as a case and temperature time-series data were collected for surface water and wetland sediment of 0.2 m, 0.6 m and 1 m respectively in 3 monitor sites. Then, a one-dimensional vertical steady heat transport model was applied to estimate surface water inflows to groundwater. The results showed that the leakage rates were 0.04-0.29 mm/d and had a spatial and temporal variation. In the same monitor site, the leakage rate was higher in winter than it in summer. A total leakage amount for the Baiyangdian wetland was evaluated as 8.61 million m3/d. The surface water leakage resulting from the model was subject to several uncertainties including sediments inhomogeneity and the impact of non-ideal conditions. Despite the uncertainties of the model, when correctly applied, heat tracer method is able to provide detailed information on the spatial and temporal distribution of surface water leakage

Energy-Saving Depth Control of an Autonomous Underwater Vehicle Using an Event-Triggered Sliding Mode Controller

In order to improve the endurance of underwater vehicles and make it possible for the underwater vehicle to inspect long-distance water tunnels, a sliding mode control method based on event triggering is proposed for the depth control of underwater vehicles from the perspective of energy saving. Firstly, the kinematics and dynamics models of underwater vehicle dive surface are established. Secondly, an event-triggered sliding mode controller is designed. According to the Lyapunov function, the stability of the designed controller is proved by theoretical analysis, and Zeno phenomena will not appear in the closed-loop control system. Compared with other controllers, the simulation results show that this controller can effectively realize the depth control of AUV, has strong adaptability and robustness to unmodeled nonlinear dynamics and bounded disturbances, and has the effect of saving computing resources

Response characteristics of water level dynamic of the Baiyangdian Wetland to climate change and human activities

Water level dynamics of Wetlands are influenced by both climate change and human activities. Understanding the influence characteristics is important for the management of wetland water resources and ecology. Based on the water level dynamic and precipitation of the Baiyangdian Wetland, and the NPI index, the paper analyzes the response characteristics of the water level dynamic to the precipitation, and the teleconnections between the water level dynamic and the NPI by adopting method of wavelet analysis. Results show that climate change plays an important role on the response of the water level dynamic to the precipitation and also to the NPI, and human activities can significantly change the response characteristics. The response time lags of the water level dynamics to the NPI is longer than that of the water level dynamics to the precipitations, which indicates that the wetland precipitation might be partially influenced by the NPI. The knowledge of the response characteristics obtained in the paper is beneficial for water conservancy and control of the wetland, and is favorable for the sustainable development of its eco-environment