Extraction of Campomanesia xanthocarpa fruit using supercritical Co2 and bioactivity assessments

This study reports extraction of Campomanesia xanthocarpa fruit using supercritical CO2 and its chemical composition and biological activity analysis. The experiments were carried out at 15.0, 20.0 and 25.0 MPa and 313.15, 333.15 and 353.15 K. The maximum yield observed was 3.90 wt% at 313.15 K and 25 MPa. The pressure had a significant and positive effect on extraction yields, whereas the temperature effect was significant and negative. Gas chromatography analysis showed that the main compounds identified were alpha-eudesmol, beta-eudesmol, gamma-eudesmol, caryophyllene (E), alpha-sabinene, beta- sabinene, germacrene B, delta-cadinene, humulene and selina-3,7(11)-diene. The highest values of antioxidant activity were found for the extracts obtained at (313.15 K and 25 MPa) and at (353.15K and 15.0 MPa), determined by the DPPH method and the phosphomolybdenum reduction method, respectively. The extract obtained at 353.15K and 25 MPa presented the highest phenolic content (39.12 mg GAE/g of extract). The extract obtained at 313.15 K and 15 MPa presented the maximum antibacterial activity against Staphylococcus aureus. The Gram-negative bacteria tested (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimuruim) and Listeria monocytogenes were completely resistant toward the C. xanthocarpa fruit extracts987985CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES485689/2012-0; 303573/2012-0sem informaçãoFundacao Araucari

Seasonal variation of the (E)-nerolidol and other volatile compounds within ten different cultivated populations of baccharis dracunculifolia DC (asteraceae)

Initially, the seeds of Baccharis dracunculifolia were collected from populations of 10 different regions, and the cultivation experiment was carried out in an experimental area of 1,800 m2 by cultivating 100 individuals of each population. The essential oil analyses were performed on both GC-FID and GC/MS, which allowed the identification of 14 compounds. The oil yield varied from 0.31% to 0.70% among populations and season. The major oxygenated sesquiterpenes in the cultivated experiment were (E)-nerolidol (32%) and spathulenol (17%). The mean concentration in the plant of (E)-nerolidol was five times higher in March (136.53 mg/100 g of plant) than it was in July (25.03 mg/100 g of plant). The mean concentration of spathulenol increased about three fold from July (16.25 mg/100 g of plant) to April (47.50 mg/100 g of plant)214308314FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP04/13005-1; 04/07331-3; 01/14219-

Seasonal Variation of the (E)-Nerolidol and Other Volatile Compounds Within Ten Different Cultivated Populations of Baccharis dracunculifolia DC (Asteraceae)

Initially, the seeds of Baccharis dracunculifolia were collected from populations of 10 different regions, and the cultivation experiment was carried out in an experimental area of 1,800 m(2) by cultivating 100 individuals of each population. The essential oil analyses were performed on both GC-FID and GUMS, which allowed the identification of 14 compounds. The oil yield varied from 0.31% to 0.70% among populations and season. The major oxygenated sesquiterpenes in the cultivated experiment were (E)-nerolidol (32%) and spathulenol (17%). The mean concentration in the plant of (E)-nerolidol was five times higher in March (136.53 mg/100 g of plant) than it was in July (25.03 mg/100 g of plant). The mean concentration of spathulenol increased about three fold from July (16.25 mg/100 g of plant) to April (47.50 mg/100 g of plant).Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Brazil[04/13005-1]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Brazil[04/07331-3]Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) Brazil[01/14219-7