A Telehealth Palliative Care Objective Structured Clinical Examination

Problem: Palliative care is a service focused on symptom management and improved quality of life for persons with serious or chronic illnesses and is widely underutilized. Although advanced practice registered nurses (APRNs) possess a unique skillset to contribute to palliative care, there is minimal reporting on palliative care training for this professional group. The purpose of this clinical scholarship project was to implement the End-of-Life Nursing Education Consortium (ELNEC) online training program for APRN students in the BSN-DNP program and evaluate its effect on student performance during a telehealth objective structured clinical examination (OSCE). Methods: An observational, descriptive design at a Midwestern public university’s college of nursing. This quality improvement initiative implemented the online ELNEC training program into a BSN-DNP curriculum. The BSN-DNP students who had completed at least one diagnosis and management course were evaluated during an OSCE for their ability to communicate and explain palliative care. Results: A total of 85 BSN-DNP students (N = 85) completed the telehealth palliative care OSCE. Scores for students’ explanation of palliative care were significantly higher among students who had completed two diagnosis and management courses when compared to those who had completed one course for their populations of focus (p = .048). Implications for Practice: The ELNEC online palliative care training resulted in BSN-DNP students successfully explaining and communicating the role of palliative care during simulation

Extreme limb profiles of the sun at far-infrared and submillimeter wavelengths

Thirty, 50, 100, and 200 microns solar limb intensity profiles determined with arcsecond resolution from airborne observations of the occultation of the solar limb during the total eclipse of 1981 July 31 are presented. Two points of particular importance emerge: (1) the longer-wavelength (100 and 200 micron) limbs are significantly brighter than disk center. At 200 microns the extreme limb is about 1.22 times the brightness of disk center. This is consistent with the 6000 K temperature-plateau structure of the model chromospheres of Vernazza, Avrett, and Loeser (1973, Ap. J., 184, 605; 1981; Ap. J. Suppl., 45, 635;) and (2) the longer wavelength limbs are extended significantly further above the visible limb than Vernazza, Avrett, and Loeser predict. These results provide a strong basis for modeling of the solar chromosphere free from the assumption of gravitational-hydrostatic equilibrium

Submillimeter extensions of the solar limb determined from observations of the total eclipse of 1981 July 31

First results are presented of observations of a lunar occultation of the solar limb made from the Kuiper Airborne Observatory in the 30 micrometr, 50 micrometer, 100 micrometer, and 200 micrometer continuum during the total solar eclipse of 1981 July 31. The solar limb was extended at the longer wavelengths up to 1000 km higher than predicted from smooth plane-parallel chromospheric models. Results at both second and third contact show the infrared limb extensions to be approximately 0".8, 1"5, 2".5 and 3".0 above the visible limb in the observed bands, respectively. A possible interpretation proposes chromospheric fine structure inhomogeneities of greater density than presently incorporated in models of the middle chromosphere

Multi‐omics analyses reveal epigenomics basis for cotton somatic embryogenesis through successive regeneration acclimation process

Plant regeneration via somatic embryogenesis is time‐consuming and highly genotype‐dependent. The plant somatic embryogenesis process provokes many epigenetics changes including DNA methylation and histone modification. Recently, an elite cotton Jin668, with an extremely high regeneration ability, was developed from its maternal inbred Y668 cultivar using a Successive Regeneration Acclimation (SRA) strategy. To reveal the underlying mechanism of SRA, we carried out a genome‐wide single‐base resolution methylation analysis for non‐embryogenic calluses (NECs), ECs, somatic embryos (SEs) during the somatic embryogenesis procedure and the leaves of regenerated offspring plants. Jin668 (R4) regenerated plants were CHH hypomethylated compared with the R0 regenerated plants of SRA process. The increase of CHH methylation from NEC to EC were demonstrated to be associated with the RNA‐dependent DNA methylation (RdDM) and the H3K9me2‐dependent pathway. Intriguingly, the hypomethylated CHH differentially methylated regions (DMRs) of promoter activated some hormone‐related and WUSCHEL‐related homeobox genes during the somatic embryogenesis process. Inhibiting DNA methylation using zebularine treatment in NEC increased the number of embryos. Our multi‐omics data provide new insights into the dynamics of DNA methylation during the plant tissue culture and regenerated offspring plants. This study also reveals that induced hypomethylation (SRA) may faciliate the higher plant regeneration ability and optimize maternal genetic cultivar

Cotton pan-genome retrieves the lost sequences and genes during domestication and selection

Background Millennia of directional human selection has reshaped the genomic architecture of cultivated cotton relative to wild counterparts, but we have limited understanding of the selective retention and fractionation of genomic components. Results We construct a comprehensive genomic variome based on 1961 cottons and identify 456 Mb and 357 Mb of sequence with domestication and improvement selection signals and 162 loci, 84 of which are novel, including 47 loci associated with 16 agronomic traits. Using pan-genome analyses, we identify 32,569 and 8851 non-reference genes lost from Gossypium hirsutum and Gossypium barbadense reference genomes respectively, of which 38.2% (39,278) and 14.2% (11,359) of genes exhibit presence/absence variation (PAV). We document the landscape of PAV selection accompanied by asymmetric gene gain and loss and identify 124 PAVs linked to favorable fiber quality and yield loci. Conclusions This variation repertoire points to genomic divergence during cotton domestication and improvement, which informs the characterization of favorable gene alleles for improved breeding practice using a pan-genome-based approach

Human Claspin Is a Ring-shaped DNA-binding Protein with High Affinity to Branched DNA Structures

Claspin is an essential protein for the ATR-dependent activation of the DNA replication checkpoint response in Xenopus and human cells. Here we describe the purification and characterization of human Claspin. The protein has a ring-like structure and binds with high affinity to branched DNA molecules. These findings suggest that Claspin may be a component of the replication ensemble and plays a role in the replication checkpoint by directly associating with replication forks and with the various branched DNA structures likely to form at stalled replication forks because of DNA damage