Effects of Bisphosphonates on Root Resorption during Experimental Tooth Movement

The aim of the present study was to investigate the effect of the local administration of three kinds of bisphosphonates -etidronate, alendronate, and pamidronate- on orthodontic root resorption. Both the right and left molars of 7-week-old male Sprague Dawley rats were experimentally moved by interproximal insertion of the elastic band according to Waldo's method. Each bisphosphonate solution was injected into the palatal subperiosteum area adjacent to the upper first molar every third day during experimental period. Rats were sacrificed after 1, 3, 7, and 14 days of elastic band insertion, and samples prepared from tissues were histomorphometrically analyzed by light or scanning electron microscopy (SEM). The SEM analysis revealed that the local injection caused a significant reduction in mechanically induced root pit formation in all bisphosphonate treatment groups after 14 days. The root pit formation was parallel with the reduction in the number of odontoclasts in histological examination for tartrate-resistent acid phosphatase activity. However, on day 3, treatment with etidronate and alendronate transiently increased the root pit formation due to the increase of the odontoclast number. Immunohistochemical study was performed to examine the effect of these bisphosphonates on the expression of proinflammatory cytokines, i.e., interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). On day 3, in etidronate- and alendronate-trearment groups, the strong immunoreactive signals of IL-1β and TNF-α were observed in odontoclasts, osteoclasts, and periodontal ligament cells compared with control groups. Pamidronate treatment did not affect the expression of IL-1β, TNF-α, and IL-6 during whole experimental period. Thus, topical application of bisphosphonates following experimental induction of tooth movement in rats reduced the root resorption and the number of odontoclasts. These results suggest that bisphosphonates may be useful for prevention of root resorption of teeth during orthodontic tooth movement. Moreover, the effect of etidronate and alendronate via IL-1β and TNF-α expression might be associated with odontoclast and osteoclast formation

COMPARISON FOR CARICA PAPAYA AND GENGIGEL LEAVES EXTRACTION FOR GINGIVITIS HEALING EFFECTIVENESS IN ORTHODONTIC APPLICATION

Background: Gingivitis is the infection disease with tooth loss effect. Using fixed orthodontic appliance in dentistry can give gingivitis impact with its’ designed way. To determine the effectiveness of papaya leaf extraction and gengigel in gingivitis treatment with fixed orthodontic application in letrature review explanation Methods: Literature searching with Science Direct, NCBI (PubMed), and Google Scholar databases and getting 530 articles. Articles are screened to be 67 articles and selected by inclusion and exclusion criteria getting 37 articles. 37 Articles are ready to be analysed. Results:There is effectiveness of papaya leaf extract and gengigel in patients using fixed orthodontic appliances for giving anti inflammation effect, wound healing and enhance blood circulation. Conclusion: The conclusion is papaya and gengigel leaf extraction giving gingivitis healing effect in fixed orthodontic treatmen

PERBEDAAN EFEKTIVITAS CHLORHEXIDINE GLUKONAT 0,2% DENGAN TEH HIJAU (CAMELLIA SINENSIS) TERHADAP JUMLAH PORPHYROMONAS GINGIVALIS

Background: Periodontitis is a periodontal inflammation caused by plaque that contains pathogens, Porphyromonas gingivalis is one of them. Chlorhexidine therapy used to reduce the number of pathogenic bacteria causing periodontitis. Green tea contains polyphenols such as epigallocatechin-3-gallic as antibacterial agent that can kill the bacteria Porphyromonas gingivalis. This study aims to determine differences in the effectiveness of chlorhexidine gluconate 0.2% with green tea (Camellia sinensis) various concentrations to decrease the amount of bacteria Porphyromonas gingivalis.Method: This research was experimental with post test only design. Method used in antibacterial test was a drop plate misra. The experimental group consisted of six groups: chlorhexidine gluconate 0.2% and green tea group with a concentration of 100%, 75%, 50%, 25% and 12.5%. minimum bactericidal concentrations againts Porphyromonas gingivalis was evidenced by counting the number of colonies that formed on agar. Analysis data was using One Way ANOVA continued by Post hoc tests Tamhane.Result: The results showed that chlorhexidine gluconate 0.2% with green tea effective to decrease the amount of bacteria Porphyromonas gingivalis(ANOVA p <0.05). The results between the groups showed green tea 100%, 75% and 25%, have same effect compared to chlorhexidine gluconate 0.2% in reducing Porphyromonas gingivalis.Conclusion: In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis

BAKTI SOSIAL PENINGKATAN PENGETAHUAN KESEHATAN GIGI DALAM MENYUKSESKAN PROGRAM USAHA KESEHATAN GIGI MASYARAKAT (UKGM) DI DESA DEMPET DEMAK

Background: Pemeliharaan kebersihan gigi dan mulut merupakan salah satu upaya meningkatkan Kesehatan. Di desa Dempet masih banyak dijumpai keadaan giginya yang karies dan kebersihan mulutnya masih rendah. Melakukan upaya promotif dan preventif sehingga dapat meningkatkan kesadaran dan pengetahuan tentang pentingnya perilaku menjaga kesehatan gigi dan mulut.Method: Metode yang digunakan untuk meningkatkan pengetahuan dan kesadaran masyarakat berupa penyuluhan, diskusi interaktif dan pemutaran video tentang bagaimana cara menjaga Kesehatan gigi mulut.Result: Pengetahuan masyarakat terhadap kesehatan gigi meningkat, terbentuknya sikap masyarakat yang perduli terhadap kesehatan gig Conclusion: Penyuluhan dan video edukasi sangat efektif dalam meningkatkan pemahaman masyarakat tentang kesehatan gigi

Psychological analysis of mothers with cleft children

Background: Parenting a child with cleft disorder required special attention, because this is related to the psychological condition of the mother. Mother's psychological problems can affect the growth and development of children. The purpose of this study was to analyze psychological of mothers with cleft children. Method: The research method used in this research is descriptive analytic method with purposive sampling approach. Methods of data collection using a questionnaire to measure parenting stress, the short form parenting stress index (PSI) had adopted from Adibin has been tested for validity and reliability. The number of samples in this study were 150 mothers with cleft lip, palatal and cleft lip/palatal children.Statistic analysis with ANOVA test and Spearman test Result: The results of this study indicate that there are differences in the stress levels of mothers of cleft children between cleft lips (CL), cleft palate (CP) and cleft lips and palate (CL/P) non syndromic (NS). The significance value is 0.000 (

THE INFLUENCE OF LEMON (Citrus Limon (L.)) IRON ION (Fe) REMOVAL ON STAINLESS STEEL ORTHODONTIC WIRE

Background: Lemons consist of 5-8% citric acid, have a pH of around 2.74. Drinks that have a critical pH of 5.5 can be said to be acidic drinks. Acidic drinks have the potential to cause corrosion of teeth and dental materials, one of which is stainless steel orthodontic wire. Stainless steel orthodontic wire is easily corroded, the wire has a composition of 71% Iron (Fe), 18% Chromium (Cr), 8% Nickel (Ni), and 0.2% Carbon (C). Corrosion is caused by the presence of inorganic components that act as electrolyte media that can trigger electrochemical reactions.Method: Each sample was placed on each uninsulated petridish and labeled as a marker. This is done by inserting orthodontic wire which is immersed into the incubator for 3.5 hours at 37℃. Then the sample is taken and the separation between the sample and the solution is carried out. After that, the measurement of Iron (Fe) ions was carried out using the Atomic Absorption Spectrophotomery tool.Result: The results showed the average release of Iron (Fe) ions in the experimental group of lemon juice with a concentration of 25% was 0.067mg/L, a concentration of 50% was 0.090mg/L and a concentration of 100% was 0.135mg/L. The test results obtained using the One Way Anova test showed that there was no significant difference (p>0.05).Conclusion: There was no significant difference in the release of Iron (Fe) ions in stainless steel orthodontic wires between experimental groups, and there was no effect of soaking lemon juice (Citrus Limon (L.)) on the release of Iron (Fe) ions in stainless steel orthodontic wires

CARIES STATUS EARLY CHILDHOOD CARIES IN INDONESIAN CHILDREN WITH SPECIAL NEEDS : Study In SDLB Central Java

Background: The prevalence of ECC (Early Childhood Caries) is still problem in Indonesian, especially in children with special needs. Unstable economic development leads to a poorer economic situation and may have an impact on increasing the prevalence of ECC. This study aimed to investigate the Caries Status of ECC in children with special needs and its association with sociodemografi and parental socioeconomic circumstances in Semarang, Central Java, Indonesian.Method: This research was conducted in SDLB using questionnaire and Using World Health Organization for diagnosis of caries. Informed consent from parents was obtained. The study sample was 21 children aged 3-6 years which consisted of 12 boys and 9 girls. Data were tested by Spearman correlation. Result: The result showed that 85.7% of the children had ECC. The result also showed significant association between ECC in the child and the age of the mother (r= 0.453, p=0.018), education level of the mother (r=0.741, p=0.0001), social status (r=0.807, p=0.0061) and other income (r=0.527, p=0.050). Conclusion: The conclusion of this study is that there was a significant association between caries status of a young child and the age of the mother, the socio-economic status and other income

METRONIDAZOLE GEL EFFECT ON RATS WITH BACTERIA-INDUCED PERIODONTITIS

Background: Periodontitis is an inflammatory disease in the periodontal tissue and causes progressive tissue damage. Tissue Inhibitor of Matrix Metalloproteinases-1 (TIMP-1) is directly responsible for the pathogenesis of periodontal diseases. Topical application of the metronidazole gel as a therapy for periodontal diseases can be used for additional therapy. This study aimed to analyze the effect of Metronidazole gel on periodontitis based on TIMP-1 levels in bacteria-induced periodontitis in rats. Methods: Twenty Sprague Dawley rats weighing 170-200 gram aged two months. Rats were divided into two groups: the control group and Metronidazole gel (Ti-es Metronidazole gel, 0.45 mg). Metronidazole gel was topically applied to the gingival sulcus. The gingival crevicular fluid sample was taken on day 3 with periopaper subjected to the Tissue Inhibitor of Matrix Metalloproteinase-1 level evaluation using the ELISA Method. Results: Normal reference Tissue Inhibitor of Matrix Metalloproteinase 1 levels gingival crevicular fluid 0.156-10 ng/ml. Tissue Inhibitor of Matrix Metalloproteinase 1 gingiva crevicular fluid in control and metronidazole gel was 1.34 ng/ ml and 3.57 ng /ml. There was a significant difference between control and metronidazole gel group (p<0.005)Conclusion: The Application of Metronidazole gel affects TIMP-1 levels in periodontitis

EFEKTIVITAS MENYIKAT GIGI DENGAN METODE BASS DAN HORIZONTAL TERHADAP PERUBAHAN INDEKS PLAK PADA ANAK TUNAGRAHITA

Background: The important aspect for child especially children with mental retardation is oral health. Children with mental retardation have limitations in motoric activities that relate to the actions of dental hygiene in normally, that activities is toothbrushing and it can do by bass and horizontal method. The aim of this study is to know the difference effectiveness toothbrushing with bass and horizontal method on the changed of plaque index in children with mental retardation. Method: The quasi experimental methods with non-equivalent group design with post test design was used in this research and have done on 24 children with mild mental retardation at SDLB Purwosari Kudus which divided two groups, 12 students brushing with bass method and 12 students brushing with horizontal method. Result: The data were tested with paired t-test to compare plaque index before and after toothbrushing with bass and horizontal method, showed that there were meaningful differences (p0,05). Conclusion: It concluded that both bass and horizontal method effectively on the changed of plaque index in children with mental retardation. There was no difference effectiveness between both methods. Keywords: bass method, horizontal method, plaque index, children with mental retardatio

ANALYSIS STUDENT CONSELOR KNOWLEDGES ABOUT TOOTH IMPACTION IN THE HIGH SCHOOL TEGAL

Background: The problem of impacted teeth is often asked by high school students. A student conselor must be provided with knowledge related to impacted teeth and their impact. The purpose of this study was to determine the level of knowledge of student conselors about impacted teeth.Method: Questionnaires were distributed to 40 student conselors, before and after counseling, then the differences were analyzed using SPSS.Result: Based on statistical calculations, the p value of 0.000 (p <0.05) means that there was a significant difference between the knowledge of student conselors before and after counseling. Conclusion: there was a difference in tooth impaction knowledges between before counseling and after counselin