KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL

The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12)

Penerapan Teknik Citra Digital Sebagai Metode Pengukuran Morfometrik Ternak Pada Sapi Bali dan Peranakan Ongole

Morphometric measurements using digital image technique give a lot of benefits i.e., a safer method both for the researchers and the experimental animals, produce a more accurate morphometric data, reduce the stress in the experimental animals and produce a more detail and complete morphometric data. This experiment used 20 Ongole cross cattle and 20 Bali cattle with the average age of 3 years. Digital image data were collected by using DSLR camera with 18Mp resolution. The collected digital image data were analyzed by using Image J program. Parameters measured in this observation were body length, body height, hip height, pelvic height, loin height, the length of ossa vertebrae cervicales, the length of ossa vertebrae thoracicae, the length of ossa vertebrae lumbales, the length of os scapula, the length of os humerus, the length of ossa radius-ulna, the length of os metacarpale III, the length of os femoris, the length of ossa tibia-fibula, and the length of os metatarsale III. Digital image technique could replace the manual technique for morphometric measurement with a lower level of variance (<10%). Application of digital image technique for measurement of morphometric parameters in Ongole Cross and Bali cattle showed that Bali cattle has a potential for muscle deposition in body frame and body axis while Ongole Cross cattle has a potential for muscle deposition in fore and hind legs

KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL

The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12)

Distribusi Otot, Lemak dan Tulang pada Karkas Kambing Jantan Peranakan Etawah yang Diberi Suntikan Clenbuterol (DISTRIBUTION OF MUSCLE, FAT AND BONE IN MALE ETAWAH CROSS BREED BUCKS TREATED WITH CLENBUTEROL)

Twenty growing cross breed etawah buck had been treated with clenbuterol every two days. Threelevels of clenbuterol, 5, 10 and 20 ìg/kg BW were injected intramuscularly from the thigh side every twodays. The animals were slaughtered and cut into five pieces, thorax, fore limb, loin, flank and hind limb.The components of muscle and fat for each component were separated for fat, muscle and bones analysis.The purpose of the experiment was to investigate the claim that clenbuterol has an effect on carcassquality. It is expected that clenbuterol will affect fat mobilization through the mechanism of stimulusrespond . In this experiment clenbuterol does not affect fresh carcass weight and carcasss empty bodyweight. However clenbuterol has the effect on muscle carcass thigh, thorax and flank, indicating proteinformation in the carcass. Further studies showed that clenbuterol affected the reduction of subcutanousfat in thigh and flank. Intermuscular fat of the thorax was also affected by clenbuterol. Furthermoreclenbuterol reduced the bones weight. It can be concluded that clenbuterol works at specific area of thebody, mobilizing the fat and bones follow with protein synthesis

Pengukuran Morfometrik Sapi Peranakan Ongole dan Kerbau Jantan dengan Metode Citra Digital (MORPHOMETRIC MEASUREMENT OF MALE ONGOLE CROSSBRED CATTLE AND BUFFALO BY DIGITAL IMAGE ANALYSIS )

The objectives of this study were to compare the methods of animal morphometricmeasurementsusing manual and digital image analysis methods on the identify of ongole crossbred cattle and buffalo. Asmany as38 maleongole crossbred cattle and 44 male buffalos were used in this study. Fifteen bodyparameters were examined and analyzed and the data obtained were analyzed by t-student to determinethe differences between of two measurement methods. The morphometric measurement of ongole crossbredcattle and buffalo by manual was not significantly different (P>0.05) as compared to that of by digitalimaging. Therefore the digital imaging analysis method could be used as an alternative morphometricmeasurement forlarge ruminant animalal such as cattle and buffalos. Based on body morphometric bydigital image analysis showed that ongole crossbred cattle has ossa radius-ulna, os metacarpale, ossatibia fibulla, os metatarsale, hip height and body height longer than buffalo (P<0,05). Depth chest ofbuffalo deeper than ongole crossbred cattle (P<0,05). Body measurement of livestock animal by digitalanalysis method could be applied as it has similar accuracy with the manual method. Ongole crossbredcattle has good potency as beef cattle because it has higher body size proportion than buffalo

KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL

The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12)