AMELIORATIVE EFFECTS OF DIFFERENT PARTS OF GEMOR (NOTHAPHOEBE CORIACEA) ON CADMIUM INDUCED GLUCOSE METABOLISM ALTERATION IN VITRO

Objective: The present study was undertaken to investigate the ameliorative effect of the bark and leaves of Nothaphoebe coriacea (gemor) on Cadmium (Cd) induced glucose metabolism alteration in liver homogenate in vitro. Methods: Glucose metabolism alteration in liver homogenate was induced by the administration of Cadmium Sulphate (CdSO4) at a dose 3 mg/l. Ameliorative effect of the leaves and bark extracts was determined by assessing the concentration of glycogen, glucose and Methylglyoxal (MG). Dubois hydrolytic method was used for liver glycogen and glucose concentration estimation. Modified Dinitro Phenyl Hydrazine (DNPH) method was used for MG concentration estimation. Results: The results of this present studies showed that treatment with CdSO4 significantly decreased the levels of glycogen and MG concentration, and increased the level of glucose in liver homogenate compared to control. The aqueous extracts of bark and leaves of gemor significantly increased the levels of glycogen and MG concentration, and decreased the level of glucose in liver homogenate compared to control. The aqueous extracts of the bark of gemor in comparison with CdSO4 treatment group showed the significant effect to maintain the glycogen, glucose, and MG concentration in liver homogenate. However, when compared to the aqueous extracts of leaves of gemor the result was not significant. The results suggest aqueous extracts of the bark of gemor was more effective to prevent the glucose metabolism alteration induced by CdSO4 than the aqueous extracts of leaves of gemor. Conclusion: The present study demonstrated Cd could induced the glucose metabolism alteration in liver homogenate, and the aqueous extracts of bark and leaves of gemor showed the ameliorative effect to prevent this alteration. In addition, the bark was more effective than leaves of gemor to prevent the glucose metabolism alteration induced by Cd

The effect of usability, information quality, and accessibility on the intensity of the madrasa website use at Madrasah Aliyah level

The majority of academic communities in madrasa access the madrasa website infrequently. It is related to the user’s perception of the madrasa website’s quality. This study seeks to determine how usability, information quality, and accessibility effect the frequency with which madrasa websites are visited. This study proposes a correlational quantitative research methodology. The research cohort consisted of 2401 MAN Surakarta students, and the sample size was 96. The method of sampling is proportional random sampling. Data collection techniques using a questionnaire. Techniques of data analysis utilizing correlation analysis and single regression to test the hypotheses of single correlation, correlation, and multiple regression. The results of the study indicate that: 1) Usability has a positive effect on the intensity of website use; 2) Information quality has a positive effect on the intensity of website use; 3) Accessibility has a positive effect on the intensity of website use; and 4) Usability, information quality, and accessibility all have a positive effect on the intensity of website use. Usability, information quality, and accessibility collectively contribute 51.1% to the website's intensity, while the remaining 48.9% is influenced by unmeasured variables

Studi Tingkat Keanekaragaman Hayati Lahan Bekas Terbakar di Taman Nasional Sebangau & Kawasan Hutan Dengan Tujuan Khusus (KHDTK) Tumbang Nusa

The forest and land fires in 2015 caused severe damage to forest areas at Sebangau National Park and KHDTK Tumbang Nusa, where the condition has not to be able to recover as before. This research objective is knowing the effect of forest and land fires on vegetation and the succession process that is happened at TN. Sebangau and KHDTK Tumbang Nusa with methodology field survey and making plots (PU) on the burnt and un-burnt forest for the comparison. Data observation and collection were done appropriately with the vegetation strata, namely the level of seedlings, saplings, poles, and trees. The data analysis used quantitative with calculating the Species Dominance, Species Diversity, Species Richness, and Species Evenness.The research result shows the un-burnt land was observed in TN. Sebangau there are 29 vegetation species, meanwhile the burnt land there are found only rejuvenation level plants, namely Seedlings and Saplings where there are two vegetation species at TN. Sabangau and 4 vegetation species at KHDTK. For species dominance is indicated by a high INP value, the greater value of INP means the forest composition is good enough in terms of density and frequency of species presence. In Sebangau, the highest value of dominance is Tutup Kabali (Diospyros pseudomalabarica) for the tree level, Rambutan Hutan (Nephelium lappaceum) for pole and sapling levels, and Bangkinang (Elaeocarpus glaber Bl) for seedling level, while in KHDTK is Milas/Tumeh (Combretacarpus rotundatus) for the seedling and sapling levels, and Gerunggang (Cratoxylon arborescens) for pole and tree levels. The Analysis Number of species diversity, richness, and evenness species in the two research locations do not show a high value which from data processing result shows the range is low to moderate, nothing high.On burnt land, the succession process happens very slowly because it experienced the severe degradation of land causing the erosion/ subsidence of soil surface so that the forest floor was flooded as the impact of the open canopy and no shade from the bush that dominated

Perbedaan Kadar Glukosa, Kadar Insulin, Homa IR Dan Protein Scube2 Pada Penderita DM Tipe 2 Terkontrol Dan Tidak Terkontrol

Chronic diabetes mellitus is carried on by a rise in blood glucose levels as a result of decreased insulin sensitivity to manage blood sugar levels. The purpose of this study was to analyze participants with controlled and uncontrolled type 2 diabetes in relation of blood glucose, insulin, Homa-IR, and SCUBE2 protein levels. This study has a cross-sectional design and is descriptive analytic research. The sample for this study was stored biological fluid which was collected by total sampling from the Roemani Muhammadiyah Hospital Semarang Laboratory Installation in September 2022 to October 2022. The T-Independent test and the Mann-Whitney U-test used to the data analysis. The results of the T-Independent test results suggest that there was no significant variance between the insulin levels in DMT2 patients who were in control and those who unable (p=0.820, p>0.05). According to the results of the Mann-Whitney U-test analysis, there was no significant difference in the levels of scube2 protein between controlled and uncontrolled T2DM, and there was a significant difference in the glucose levels and Homa-IR values between controlled and uncontrolled T2DM, including both.but there was a significant effect of differences in glucose levels and Homa-IR values. The conclusion is that there are differences in glucose levels and Homa-IR values in controlled and uncontrolled DMT2 and there is no difference in insulin and scube 2 protein levels in controlled and uncontrolled DMT2.  Keywords: Glucose, Insulin, Homa-IR, Protein Scube

Potensi Simpanan Karbon pada Beberapa Tipologi Hutan Rawa Gambut di Kalimantan Tengah

Akurasi pendugaan simpanan karbon hutan rawa gambut dapat ditingkatkan melalui pengukuran masing-masing gudang/sumber karbon dan berbagai macam tipologi hutannya. Pengukuran tersebut berkaitan dengan besarnya kandungan dan fraksi simpanan karbon pada masing-masing gudang karbon. Penelitian ini bertujuan untuk mendapatkan kandungan dan potensi simpanan karbon pada masing-masing gudang karbon di tipologi gambut. Pengukuran simpanan karbon dilakukan pada lima gudang karbon yakni vegetasi (tingkat permudaan pohon), serasah, tumbuhan bawah, nekromasa dan tanah. Hasil penelitian menunjukkan kandungan karbon adalah 50% dari berat kering biomassa. Kandungan karbon tidak dipengaruhi oleh gudang karbon dan tipologi gambut. Pada tanah gambut, kedalaman gambut mempengaruhi besarnya kandungan karbon sehingga besarnya faktor konversi harus memperhatikan kedalaman masing-masing tipologi gambut. Potensi simpanan karbon terbesar pada tipologi hutan sekunder dengan kedalaman gambut antara 3-3,5 m sebesar 3.722,08 Mg/ha sedangkan potensi simpanan karbon terendah pada tipologi semak belukar dengan kedalaman gambut 3-3,5 m sebesar 2243,49 Mg/ha. Pada hutan gambut, gudang karbon tanah menyumbang >95% dari simpanan karbon total. Gudang karbon nekromasa memberikan sumbangan simpanan karbon terkecil. Fraksi simpanan karbon pada masing-masing gudang karbon berturut-turut adalah tanah> vegetasi> serasah> tumbuhan bawah> nekromasa.Carbon Stocks Potential of Peatland Forests Typologies in Central KalimantanAbstractAccuracy of carbon stocks estimation can be enhanced by measuring each carbon pools in various forest peatland typologies. The carbon stocks measurement is associated with the amount of contents and fractions of carbon stocks. The research objectives were to obtain the information of carbon contents and carbon stocks potentials in each carbon pool in the peat typologies. Carbon stocks measurement was conducted in five carbon pools which were: vegetation (tree stages), litter, understory, necromass, and soil. The results showed that the carbon contents reached more than 50% of its dry weight. The carbon contents were not affected by the carbon pools and peat typologies. In the soil carbon pools, peat depth affected the amount of carbon content so that the magnitude of the conversion factor should concentrate to the depth of each peat typology. The greatest potential of carbon stocks was found in the secondary forest (3,733.08 Mg/ha) with the peat depths between 3-3.5 m, while the lowest potential of carbon stocks found in the bush typology (2243.49 Mg/ha) with the peat depths between 3-3.5 m. In the peat typology, soil carbon stocks contributed more than 95% of total carbon stocks whereas necromass carbon stocks contributed the smallest amount of carbon. The fractions of carbon stocks in each carbon pools were soil> vegetation> litter> understorey> necromass, respectively

In Vitro Multiplication of <i>Lophostemon suaveolens</i> (Sol.ex Gaertn.) Peter G.Wilson & J.T. Waterh): Peatland Tree Species for Rehabilitation

Peatlands in Indonesia are one of the world’s largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a relatively unexplored plant found in Papua’s endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland rehabilitation. Seed is one of materials for the reproduction of L. suaveolens. However, the difficulty in seed collection and the limitation in seed production has become a current problem for its cultivation. Seed multiplication by using an in vitro method would be one of the mechanisms to overcome the problem. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. After 3 months of the culture initiation stage, the elongated axillary shoots were separated from the clumps and further multiplied using Murashige and Skoog (MS) media supplemented with (1) BAP (0.5 mL/L) as single PGR, (2) NAA (0.1 mL/L) as a single PGR, and (3) a combination of two types of PGR BAP (0.5 mL/L) and NAA (0.1 mL/L). Up to an incubation period of 6 months, the efficiency of leaf axillary shoot propagation was determined by counting the number of nodule multiplication coefficient (NMC), shoot length, root length, and number of leaves (six consecutive subcultures). The higher the NMC, the higher the plantlets obtained, increasing shoot regeneration from nodules physiologically increasing evapotranspiration in vitro. The highest of NMC (8.4) was observed in MS medium with a combination of 0.5 mL/L BAP and 0.1 mL/L NAA (double PGRs), with the longest shoots (5.91 cm), the longest root length (8.83 cm), and the most leaves (32). When a combination of BAP and NAA were used simultaneously, the plantlets during acclimatization were the highest survived. It was concluded that MS in combination with 0.5 mL/L BAP and 0.1 mL/L NAA is the most appropriate protocol for the success of in vitro multiplication of L. suaveolens. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale. The authors acknowledge the limitations of the experimental work and recommend further work to increase the sample size and complete the field-testing phase to help verify the initial findings presented in this paper

Tissue culture of gerunggang (Cratoxylum arborescens (Vahl) Blume): multipurpose native species of Indonesian peatland

AbstractGerunggang (Cratoxylum arborescens (Vahl) Blume) is a tree native to Indonesian peatlands that has great potential as an alternative fiber-producing species for the pulp and paper industry because it is adaptable and does not have the potential to disrupt local ecosystems. The species also contains important anti-cancer compounds for the pharmaceutical industry. These industries require large quantities of raw materials to meet their needs, which cannot be met by the supply of conventional plant seedlings. Tissue culture is one of the propagation biotechnologies that can be used as an alternative to provide more efficient germplasm. In this study, we developed a protocol for gerunggang propagation using tissue culture technique. The results showed that the combination of 1 mg/l 6-benzylamino purine (BAP) and 0.5 mg/l naphthalene acetic acid (NAA) in Murashige & Skoog media provided the fastest bud break time (2 weeks before the first subculture), with the highest shoot initiation (3.0 ± 0.2 cm), and after 4 weeks of incubation, the highest auxiliary shoot elongation (7.4 ± 0.1 cm). The significant effect of this hormone concentration also increases the nodule multiplication coefficient (NMC), which is 7.5. In vitro root response on the same propagation medium showed good growth with an average root length of 4.8 ± 0.2 cm. Acclimatization of plantlets was successful under ex vitro conditions and resulted in good growth after 2 and 3 months in the greenhouse. The advantage of applying the tissue culture method to gerunggang is that it can produce about seven times more seedlings from one explant in about 4 weeks. These results will be very useful for further research in the field and require a more intensive and comprehensive study

In Vitro Multiplication of Lophostemon suaveolens (Sol.ex Gaertn.) Peter G.Wilson &amp; J.T. Waterh): Peatland Tree Species for Rehabilitation

Peatlands in Indonesia are one of the world&rsquo;s largest carbon sinks, helping to regulate greenhouse gas emissions and global climate change. Lophostemon suaveolens is a relatively unexplored plant found in Papua&rsquo;s endemic peat ecosystem that grows well in wet areas with low fertility. It is geographically dispersed and has the potential for peatland rehabilitation. Seed is one of materials for the reproduction of L. suaveolens. However, the difficulty in seed collection and the limitation in seed production has become a current problem for its cultivation. Seed multiplication by using an in vitro method would be one of the mechanisms to overcome the problem. We present an efficient and reproducible protocol for in vitro multiplication of plantlets using nodal segments and shoot apices collected from plantlets. After 3 months of the culture initiation stage, the elongated axillary shoots were separated from the clumps and further multiplied using Murashige and Skoog (MS) media supplemented with (1) BAP (0.5 mL/L) as single PGR, (2) NAA (0.1 mL/L) as a single PGR, and (3) a combination of two types of PGR BAP (0.5 mL/L) and NAA (0.1 mL/L). Up to an incubation period of 6 months, the efficiency of leaf axillary shoot propagation was determined by counting the number of nodule multiplication coefficient (NMC), shoot length, root length, and number of leaves (six consecutive subcultures). The higher the NMC, the higher the plantlets obtained, increasing shoot regeneration from nodules physiologically increasing evapotranspiration in vitro. The highest of NMC (8.4) was observed in MS medium with a combination of 0.5 mL/L BAP and 0.1 mL/L NAA (double PGRs), with the longest shoots (5.91 cm), the longest root length (8.83 cm), and the most leaves (32). When a combination of BAP and NAA were used simultaneously, the plantlets during acclimatization were the highest survived. It was concluded that MS in combination with 0.5 mL/L BAP and 0.1 mL/L NAA is the most appropriate protocol for the success of in vitro multiplication of L. suaveolens. This is the first report of L. suaveolens in vitro multiplication, and the protocol could be used to propagate this peatland species on a large scale. The authors acknowledge the limitations of the experimental work and recommend further work to increase the sample size and complete the field-testing phase to help verify the initial findings presented in this paper