ELTROXIN LIKE MIMIC ACTION OF WITHANIA SOMNIFERA LEAF EXTRACT IN HYPOTHYROID-INDUCED RATS

Objective: The present study was designed to examine the anti-hypothyroid activity of Withania somnifera (Ashwagandha)'s leaf extract on 6-n-propyl- 2-thio-uracil (PTU) induced hypothyroid rats.Method: Ethanolic extracts of W. somnifera leaf and Eltroxin were administrated to PTU induced hypothyroid rats. The animals were divided into control, PTU treatment, W. somnifera leaf extract treatment, and Eltroxin treatment groups for 60 days. The serum T3 and T4 were estimated, and biochemical and hematological parameters of the blood serum were also evaluated.Results: PTU induction caused a significant decrease (p≤0.001) in T3 and T4 level when compared with the control group. Adverse effects of PTU were also observed in blood sugar, cholesterol, alkaline phosphate, protein, albumin, globulin, liver function test, and renal function test parameters. Non-significant changes in LFT,RFT and Other parameters were observed. W.Somnifera's leaf extract and Eltroxin treatment group recovered both of the thyroid hormone secretion as compare to control

WHEAT BRAN EXTRACT MIMICS STATIN LIKE ACTION IN HYPERLIPIDEMIC RABBITS: AN EXPERIMENTAL STUDY

 Objective: The study was aimed to evaluate the hypolipidemic and antioxidant effect of Triticum aestivum (wheat) bran extraction cholesterol-fed rabbits.Methods: The induced hyperlipidemic rabbits were treated with 70% ethanolic extract of wheat bran at a dose of 500 mg/kg body weight/day. The experimental protocol was designed for control, hyperlipidemic, wheat bran extract, and statin-treated groups for 60 days. The estimations for serum lipid profile, lipid peroxidation (LPO), and ferric reducing ability of plasma (FRAP) were done, and histopathological study of thoracic aorta was carried out.Results: Oral administration of wheat bran extract caused significant reductions in the serum lipid profile by 94.30% (total cholesterol), 96.73% (low-density lipoprotein cholesterol), and 58.28% (triglycerides). It significantly decreased the LPO and enhanced the antioxidant capacity (FRAP) activity as compared to the hyperlipidemic rabbits. Histopathology revealed that wheat bran extract treatment prevented the accumulation of cholesterol and regressed atheromatous plaques significantly in the aorta as compared to the hyperlipidemic group, and the results were comparable to that of the standard drug statin.Conclusion: The study exhibited that wheat bran extract controls atherosclerosis just like statin and can be used as a regular dietary supplement to manage cholesterol levels and prevent cardiovascular problems

PROTECTIVE EFFECT OF CUMIN (CUMINUM CYMINUM L.) SEED EXTRACT ON CARDIOVASCULAR SYSTEM, TOXICITY, AND HEMATOLOGY ON HYPERLIPIDEMIC RABBITS: AN EXPERIMENTAL STUDY

Objective: The present study is related with the assessment of anti-atherosclerotic efficacy of 70% EtOH extract of cumin seed in diet-induced hyperlipidemic rabbits.Methods: Rabbits were rendered hyperlipidemic by oral administration of cholesterol for 15 days. Then, the animals were treated with 70% EtOH (ethanolic extract) of cumin seed extract for 45 days (Group III). Another set of animals was treated with atorvastatin, the standard drug for 45 days. At the end of experimental period, the serum biochemical, hematological, and histological analysis of thoracic aorta was done.Results: The cumin seed extract showed contain hypolipidemic effect by reducing plasma cholesterol, low-density lipoproteins, and triglycerides level. While toxicological studies suggest no adverse effect on renal and liver function tests, hematological parameters were also observed in a normal range. Histological analysis showed that cholesterol administration caused a narrowing of the aortal lumen while treatment with 70% EtOH and atorvastatin decreased the plaque size and restored the luminal size of the aorta to normal.Conclusion: The present study suggests that commonly used culinary spice cumin seed possesses hypolipidemic and cardioprotective effect with a positive effect on serum biochemistry, histology, and hematology

CONTRACEPTIVE EFFECT OF NEEM SEED OIL AND ITS ACTIVE FRACTIONS ON FEMALE ALBINO RABBITS

Objective: In the present investigation, contraceptive efficacy of neem seed oil and its fractions have been examined in female albino rabbits to find out their contraceptive potential.Methods: The experimental protocol was designed for intact control, Neem seed oil alone and its six active fractions in eight groups for 30 days. The estimation of serum biochemistry, histometry of reproductive organs, and hematological parameters were done.Results: Oral administration of Neem seed oil alone and its Fraction IV and V causes significant reduction in weight of ovary and uterus which reflects antiestrogenic function of plant material. Significant increase in serum cholesterol and phospholipid in Neem seed oil alone and its Fraction IV and V-treated groups reflects that they may affect the intraovarian estrogen level either by inhibiting the ovarian cell function or by inhibiting follicle-stimulating hormone and luteinizing hormone secretion through hypothalamus-hypophysis axis and impaired estrogen synthesis.Conclusion: From the ongoing work, it can be concluded that Neem seed oil alone and its Fraction IV and V cause functional sterility in female albino rabbits without causing any side effect on general metabolism

GLUCOSE HOMEOSTATIC AND PANCREAS PROTECTIVE POTENTIAL OF TECOMELLA UNDULATA ROOT EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS .

Objective: The study was aimed to evaluate glucose homeostatic and pancreas protective potential of Tecomella undulata root extract in streptozotocin induced diabetic rats.Methods: The ethanolic root extract was prepared by following standard soxhlation methods. The experimental design was divided in to control and treated groups for 28 days of comparative experimental schedule. The body and organ weights, serum biochemistry, histo-pathology, hematology and toxicity profiles were assayed by following standard methods and protocols.Results: The treatment of ethanolic extract of root of T. undulata was significantly (p ≤ 0.001) reduced glucose levels at 7day, 14day, 21day and 28 days in comparison to standard drug of metformin. Correspondingly, lipid profile i.e. total cholesterol, HDL, VLDL, LDL and triglyceride were also altered significantly. Whereas, body and organs weight and hematological parameters were not shown significant changes. Subsequently, toxicity profile i.e. hepatic and renal parameters were remained under normal ranges. Corresponding, the treatment of ethanolic root extract caused normalcy of histoarchitecture of pancreas in comparison to standard drugs.Conclusion: The results of study illustrated that Tecomella undulata root extract possessing particular kind of phytocompounds which caused glucose homeostatic and pancreas protective potential in diabetic rats