Development of dysbiosis in the organism of rats receiving a high-fat diet

Background. To determine the effect on the degree of dysbiosis in the organs and tissues of rats treated with high-fat diet (HFD) using fats with various fatty acid compositions.Methods. We used ordinary (high-linoleic) sunflower oil, high-oleic sunflower oil, butter, palm and coconut oils. Rats were fed with 15 % of each of the fats for 64 days. In the blood serum from v.cava and v. porta, in the liver, heart, brain, skeletal muscles and intestinal mucosa, urease activity (an indicator of bacterial insemination), lysozyme activity (a factor of non-specific immunity) were determined, and the degree of dysbiosis was calculated from the ratio of relative urease and lysozyme activities. Results. The activity of urease in the blood of v. porta increased in rats treated with HFD, and was significantly higher than in the blood of v.cava. In most of the studied tissues, urease activity increased after HFD, with the exception of rats receiving high-oleic sunflower oil (HOSO). In contrast, lysozyme activity was reduced in most tissues, with the exception of rats treated with HOSO. The degree of dysbiosis increased after HFD with the exception of rats treated with HOSO.Conclusion. HFD increases the translocation of bacteria from the intestine. The liver partially neutralizes the microflora coming from the intestines. In blood serum from v. cava and v. porta, urease activity (an indicator of bacterial contamination), lysozyme activity (nonspecific immunity factor) were determined in the liver, heart, brain, skeletal muscle and intestinal mucosa, and the degree of dysbiosis was calculated by the ratio of the relative activities of urease and lysozyme.Results. Blood urease activity v. porta was elevated in rats treated with HFD and was significantly higher than in blood v. cava. In most of the tissues studied, urease activity increased after HFD with the exception of rats treated with high oleic sunflower oil (HOSO), the development of dysbiosis in all tissues of the body. The exception is HOSO, which does not cause the development of dysbiosis and inflammation

Оцінка клінічного перебігу запальних захворювань тканин пародонта за вмістом середньомолекулярних пептидів у біологічних рідинах в працівників агропромислових виробництв, які працюють в умовах відкритого та закритого ґрунту при впливі пестицидів

The article adduces the dynamics of concentration changes of medium molecular peptides in oral liquid and blood serum in persons with inflammatory diseases of periodontal tissue who work in different conditions of agriculture manufacture at pesticides influence. It was proved that in this contingent the increase of the marker of endogenous intoxication in biological liquids with enlargement of intensification of inflammatory processes in paradontium occurs, moreover, in people who work on closed greenhouse grounds at pesticides influence this tendency has more expressed character than in almost healthy persons of control group in patients with inflammatory diseases of periodontal tissue who don’t suffer from negative influence of harmful factors of agriculture enterprises.В статье проанализирована динамика изменений концентрации среднемолекулярных пептидов в ротовой жидкости и сыворотки крови в лиц с воспалительными заболеваниями тканей пародонта, работающих в различных условиях агропромышленных производств при воздействии пестицидов. Доказано, что у данного контингента происходит увеличение данного маркера эндогенной интоксикации в биологических жидкостях с возростанием интенсификации воспалительных процессов в пародонте, причем у работающих на закрытых грунтах тепличных хозяйств при воздействии пестицидов, эта тенденция носит более выраженный характер, чем у практически здоровых людей контрольной группы и в лиц с воспалительными заболеваниями тканей пародонта, которые не испытывают негативного влияния вредных факторов агропромышленных предприятий.У статті проаналізовано динаміку змін концентрації середньомолекулярних пептидів у ротовій рідині та сироватки крові в осіб із запальними захворюваннями тканин пародонта, які працюють у різних умовах агропромислових виробництв при впливі пестицидів. Доведено, що у даного контингенту відбувається зростання даного маркера ендогенної інтоксикації в біологічних рідинах зі збільшенням інтенсифікації запальних процесів у пародонті, причому в осіб, які працюють на  закритих ґрунтах  тепличних господарств за впливу пестицидів, ця тенденція носить більш виражений характер, ніж у практично здорових людей контрольної групи та в осіб із запальними захворюваннями тканин пародонта, які не зазнають негативного впливу шкідливих чинників агропромислових підприємств

Chiral π\pi-exchange NN-potentials: Results for diagrams proportional to g_A^4 and g_A^6

We calculate in (two-loop) chiral perturbation theory the local NN-potentials generated by the three-pion exchange diagrams proportional to g_A^4 and g_A^6. Surprisingly, we find that the total isoscalar central $3\pi$-exchange potential vanishes identically. The individually largest $3\pi$-exchange potentials are of isoscalar spin-spin, isovector central and isoscalar tensor type. For these potentials simple analytical expressions can be given. The strength of these dominant $3\pi$-exchange potentials at r=1.0 fm is 4.6 MeV, 2.9 MeV and 1.4 MeV, respectively. Furthermore, we observe that the spin-spin and tensor potentials due to the diagrams proportional to g_A^6 do not exist in the infinite nucleon mass limit.Comment: 8 pages, 5 figure

Bioinformatics tools for the discovery of new lipopeptides with biocontrol applications

As conventional or chemical pesticides have negative impact on environment and health of both farmer and consumers, it becomes relevant to develop alternative solutions to limit their use. In this context, innovative strategies to accelerate the development of biocontrol agents are welcome. For a decade of years, it has been demonstrated that lipopeptides are very efficient weapons against fungi responsible for crop diseases. Lipopeptides are secondary metabolites, produced by many microorganisms including beneficial rhizobacteria. The lipopeptide biosynthetic pathways include nonribosomal peptide synthetases. These modular enzymatic complexes work as assembly lines to build the peptides step by step, leading to the production of original peptide compounds with specific features as the presence of non proteinogenic monomers and cyclic and branched structures. In this paper, Florine and Norine bioinformatics tools, especially dedicated to non-ribosomal synthetases and their products are presented. Their use is mainly focused on the discovery of lipopeptides produced by Bacillus or Pseudomonas because they seem to represent a versatile reservoir of active secondary metabolites with promising activities for applications in phytosanitary area. © 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging.Peer reviewe

Norine, the knowledgebase dedicated to nonribosomal peptides, is now open to crowdsourcing

International audienceSince its creation in 2006, Norine remains the unique knowledgebase dedicated to non-ribosomal pep-tides (NRPs). These secondary metabolites, produced by bacteria and fungi, harbor diverse interesting biological activities (such as antibiotic, anti-tumor, siderophore or surfactant) directly related to the diversity of their structures. The Norine team goal is to collect the NRPs and provide tools to analyze them efficiently. We have developed a user-friendly interface and dedicated tools to provide a complete bioinformatics platform. The knowledgebase gathers abundant and valuable annotations on more than 1100 NRPs. To increase the quantity of described NRPs and improve the quality of associated annotations , we are now opening Norine to crowdsourc-ing. We believe that contributors from the scientific community are the best experts to annotate the NRPs they work on. We have developed MyNorine to facilitate the submission of new NRPs or modifications of stored ones. This article presents MyNorine and other novelties of Norine interface released since the first publication. Norine is freely accessible from the following URL: http://bioinfo.lifl.fr/NRP

Chiral 3π\pi-exchange NN-potentials: Results for dominant next-to-leading order contributions

We calculate in (two-loop) chiral perturbation theory the local NN-potentials generated by the three-pion exchange diagrams with one insertion from the second order chiral effective pion-nucleon Lagrangian proportional to the low-energy constants $c_{1,2,3,4}$. The resulting isoscalar central potential vanishes identically. In most cases these $3\pi$-exchange potentials are larger than the ones generated by the diagrams involving only leading order vertices due to the large values of $c_{3,4}$ (which mainly represent virtual $\Delta$-excitation). A similar feature has been observed for the chiral $2\pi$-exchange. We also give suitable (double-integral) representations for the spin-spin and tensor potentials generated by the leading-order diagrams proportional to $g_A^6$ involving four nucleon propagators. In these cases the Cutkosky rule cannot be used to calculate the spectral-functions in the infinite nucleon mass limit since the corresponding mass-spectra start with a non-vanishing value at the $3\pi$-threshold. Altogether, one finds that chiral $3\pi$-exchange leads to small corrections in the region $r\geq 1.4$ fm where $1\pi$- and chiral $2\pi$-exchange alone provide a very good strong NN-force as shown in a recent analysis of the low-energy pp-scattering data-base.Comment: 11 pages, 7 figures, to be published in The Physical Review

Teor de nitrogênio em sementes de Araucaria angustifolia.

Edição dos resumos do 18º Congresso Brasileiro de Sementes, 2013, Florianópolis

Three-pion exchange: a gap in the nucleon-nucleon potential

The leading contribution to the three-pion exchange nucleon-nucleon potential is calculated in the framework of chiral symmetry. It has pseudoscalar and axial components and is dominated by the former, which has a range of about 1.5 fm and tends to enhance the OPEP. The strength of this force does not depend on the pion mass and hence it survives in the chiral limit.Comment: 20 pages, 5 figures, 1 tabl

Estimativa de parâmetros genéticos em progênies de Cordia thricotoma.

Resum