Biomimetic Sonar for Electrical Activation of the Auditory Pathway

Relying on the mechanism of bat’s echolocation system, a bioinspired electronic device has been developed to investigate the cortical activity of mammals in response to auditory sensorial stimuli. By means of implanted electrodes, acoustical information about the external environment generated by a biomimetic system and converted in electrical signals was delivered to anatomically selected structures of the auditory pathway. Electrocorticographic recordings showed that cerebral activity response is highly dependent on the information carried out by ultrasounds and is frequency-locked with the signal repetition rate. Frequency analysis reveals that delta and beta rhythm content increases, suggesting that sensorial information is successfully transferred and integrated. In addition, principal component analysis highlights how all the stimuli generate patterns of neural activity which can be clearly classified. The results show that brain response is modulated by echo signal features suggesting that spatial information sent by biomimetic sonar is efficiently interpreted and encoded by the auditory system. Consequently, these results give new perspective in artificial environmental perception, which could be used for developing new techniques useful in treating pathological conditions or influencing our perception of the surroundings

IDH2 inhibition enhances proteasome inhibitor responsiveness in hematological malignancies

Proteasome inhibitors (PIs) are extensively used for the therapy of multiple myeloma (MM) and mantle-cell lymphoma (MCL). However, patients continuously relapse or are intrinsically resistant to this class of drugs. Here, to identify targets that synergize with PIs, we carried out a functional screening in MM cell lines using a short hairpin RNA library against cancer driver genes. Isocitrate dehydrogenase 2 (IDH2) was identified as a top candidate, showing a synthetic lethal activity with the PI carfilzomib (CFZ). Combinations of FDA approved PIs with a pharmacological IDH2 inhibitor (AGI-6780) triggered synergistic cytotoxicity in MM, MCL, and Burkitt's lymphoma (BL) cell lines. CFZ/AGI-6780 treatment increased death of primary CD138+ cells from MM patients and exhibited a favorable cytotoxicity profile towards peripheral blood mononuclear cells and bone marrow-derived stromal cells. Mechanistically, CFZ/AGI-6780 combination significantly decreased tricarboxylic acid (TCA) cycle activity and ATP levels, as a consequence of enhanced IDH2 enzymatic inhibition. Specifically, CFZ treatment reduced the expression of nicotinamide phosphoribosyltransferase (NAMPT), thus limiting IDH2 activation through the NAD+-dependent deacetylase SIRT3. Consistently, combination of CFZ with either NAMPT or SIRT3 inhibitors impaired IDH2 activity and increased MM cell death. Finally, inducible IDH2 knockdown enhanced the therapeutic efficacy of CFZ in a subcutaneous xenograft model of MM, resulting in inhibition of tumor progression and extended survival. Taken together, these findings indicate that NAMPT/SIRT3/IDH2 pathway inhibition enhances the therapeutic efficacy of PIs, thus providing compelling evidence for treatments with lower and less toxic doses and broadening the application of PIs to other malignancies

Influence of the fabrication accuracy of hot-embossed PCL scaffolds on cell growths

Polycaprolactone (PCL) is a biocompatible and biodegradable polymer widely used for the realization of 3D scaffold for tissue engineering applications. The hot embossing technique (HE) allows the obtainment of PCL scaffolds with a regular array of micro pillars on their surface. The main drawback affecting this kind of micro fabrication process is that such structural superficial details can be damaged when detaching the replica from the mold. Therefore, the present study has focused on the optimization of the HE processes through the development of an analytical model for the prediction of the demolding force as a function of temperature. This model allowed calculating the minimum demolding force to obtain regular micropillars without defects. We demonstrated that the results obtained by the analytical model agree with the experimental data. To address the importance of controlling accurately the fabricated microstructures, we seeded on the PCL scaffolds human stromal cell line (HS-5) and monocytic leukemia cell line (THP-1) to evaluate how the presence of regular or deformed pillars affect cells viability. In vitro viability results, scanning electron and fluorescence microscope imaging analysis show that the HS-5 preferentially grows on regular microstructured surfaces, while the THP-1 on irregular microstructured ones

PVDF Based Sonar for a Remote Web System to Control Mobile Robots

Wide-band ultrasound transducers, based on ferroelectric polymer technology, were used for the measurement of distances in unstructured environments. Piezo-polymer based sonar was mounted aboard mobile robot to emulate the function of bio-sonar, according to strategies observed in the flight of bats. A complete electronic system was designed and assembled, with reduced dimensions, weight, and power consumption, boasting easy assembly onto small mobile devices. Different techniques for distance measurement (threshold method and cross-correlation function together with a denoising method based on wavelet transform) are discussed and compared with results reported in literature, emphasizing the advantages of the polymer transducer in terms of versatility, efficiency, and work modalities. The Web Publishing Tool implemented the remote control features in LabVIEW to manage and modify the signal parameters during testing and acquire data

Development of an innovative superconducting magnetic energy storage system

The present work is focused on the demonstration of an innovative approach to a superconducting magnetic energy storage system by means of next generation superconducting wires. The device is thought to be integrated in a more complex biomass plant for green energy production which includes an anaerobic digester and a cogenerator for biogas and electrical energy production. Presented technology allows the storage of the green energy produced with a very high efficiency and with a better power quality respect to traditional counterparts

Anti-Reflective Zeolite Coating for Implantable Bioelectronic Devices

Since sunlight is one of the most easily available and clean energy supplies, solar cell development and the improvement of its conversion efficiency represent a highly interesting topic. Superficial light reflection is one of the limiting factors of the photovoltaic cells (PV) efficiency. To this end, interfacial layer with anti-reflective properties reduces this phenomenon, improving the energy potentially available for transduction. Nanoporous materials, because of the correlation between the refractive index and the porosity, allow low reflection, improving light transmission through the coating. In this work, anti-reflective coatings (ARCs) deposited on commercial PV cells, which were fabricated using two different Linde Type A (LTA) zeolites (type 3A and 4A), have been investigated. The proposed technique allows an easier deposition of a zeolite-based mixture, avoiding the use of chemicals and elevated temperature calcination processes. Results using radiation in the range 470–610 nm evidenced substantial enhancement of the fill factor, with maximum achieved values of over 40%. At 590 and 610 nm, which are the most interesting bands for implantable devices, FF is improved, with a maximum of 22% and 10%, respectively. ARCs differences are mostly related to the morphology of the zeolite powder used, which resulted in thicker and rougher coatings using zeolite 3A. The proposed approach allows a simple and reliable deposition technique, which can be of interest for implantable medical devices

Infrared saliva analysis of psoriatic and diabetic patient.: similarities in protein components

Psoriasis is a chronic skin disease which is very common in the population and requires frequent clinical and pharmacological treatment. In the following, a study based on Fourier transform infrared spectroscopy analyzing saliva proteomic components in psoriatic patients against diabetic patients and a control group is presented. Clinical analysis showed a prominent amide II band, at around 1545 cm(-1), and the composition of the amide I band, at around 1647 cm(-1), allowing us to distinguish the infrared salivary signature of psoriatic and diabetic patients from the control group and even from patients with different kinds of psoriasis. Moreover, results highlighted existing differences in the secondary structure composition of proteins between psoriatic and diabetic patients as compared to the control group. In fact, the saliva spectra of the control group and that of the palmoplantar psoriatic patients differ from plaque psoriasis and diabetic patient spectra because of the absence of the amide II band and the presence of different secondary protein-structure conformations

Development of Non-Invasive Ventilator for Homecare and Patient Monitoring System

Recently, the incidence of, and interest in, respiratory diseases has been amplified by severe acute respiratory syndrome coronavirus (SARS-CoV-2) and other respiratory diseases with a high prevalence. Most of these diseases require mechanical ventilation for homecare and clinical therapy. Herein, we propose a portable and non-invasive mechanical fan (NIV) for home and clinical applications. The NIV’s core is a turbine for airflow generation, which can provide and monitor a positive two-level pressure of up to approximately 500 lpm at 50 cmH2O according to the inspiration/expiration phase. After calibration, the proposed NIV can precisely set the airflow with a pressure between 4 cmH2O and 20 cmH2O, providing a versatile device that can be used for continuous positive airway pressure (CPAP) or bilevel positive airway pressure (BiPAP). The airflow is generated by a turbine monitored using a mass flow sensor. The whole NIV is monitored with a 16 MHz clock microcontroller. An analog-to-digital converter is used as the input for analog signals, while a digital-to-analog converter is used to drive the turbine. I2C protocol signals are used to manage the display. Moreover, a Wi-Fi system is interfaced for the transmission/reception of clinical and technical information via a smartphone, achieving a remote-controlled NIV

FT-IR saliva analysis for the diagnosis of psoriasis. a pilot study

Psoriasis is a chronic, autoimmune disease with multiple interplaying risk factors. Saliva has gained growing interest as an excellent biological fluid exhibiting a strong diagnostic potential in dermopathies. Saliva profiling through Fourier Transform Infrared Spectroscopy in attenuated Total Reflection (FT-IR ATR) was investigated for the diagnosis of psoriasis. Particularly, multivariate analysis was carried out after a suitable pre-processing, applying unsupervised principal component analysis (PCA) for feature extraction in the Amide I/II, Thiocyanate and within Thiocyanate and bio fingerprint bands. Further, linear discriminant analysis (LDA) and support vector machine (SVM) were trained to establish discrimination models between psoriatic subjects and healthy controls. PCA-LDA evidenced a classification performance in the bio fingerprint region (2150–900 cm− 1 ) of 93.75% accuracy, and a sensitivity and specificity of 87.5% if compared to SVM (87.5% accuracy, with a sensitivity and specificity of 75%). Saliva profiling and multivariate analysis provide a powerful approach in diagnosis and follow-up of inflammatory dermatopathies. FT-IR saliva profiling, signal processing and machine learning algorithms evidenced the possibility of automatic classification of psoriatic patients, with a potentially interesting insight in mass screening and preliminary diagnosi