Isolation, identification and molecular characterization of causative agent of bovine tuberculosis in Vojvodina region

Cilј istraživanja bio je da se izvrši izolacija, identifikacija i molekularna karakterizacija, uzročnika tuberkuloze goveda u Vojvodini, kao i ispitivanje osetlјivosti izolovanih sojeva na odabrane antituberkulozne antibiotike koji se koriste za terapiju tuberkuloze lјudi u našoj zemlјi. Pored toga cilj istraživanja bilo je i uporedno ispitivanje osetlјivosti i specifičnosti γ-IFN testa u dijagnostici tuberkuloze sa klasičnim mikrobiološkim metodama izolacije i identifikacije uzročnika. Konačno, na osnovu ispitivanja raširenosti, incidencije i prevalencije oboljenja u zapatima cilj je bila i izrada aktuelne epizootiološke karte raširenosti tuberkuloze goveda u Vojvodini. Za izolaciju bakterija MTB-kompleksa korišćeni su uzorci tkiva (pluća i limfni čvorovi) 49 goveda koja su prilikom izvođenja komparativnog tuberkulinskog ili γ-IFN testa imala pozitivnu reakciju. Kod 40 od ovih grla prilikom patomorfološkog pregleda na liniji klanja, bile su ustanovljene promene koje mogu ukazivati na tuberkulozu. Bakterije MTB-kompleksa uspešno su izolovane iz uzoraka tkiva 19 goveda, dok je nalaz acidorezistentnih bakterija ustanovljen direktnom mikroskopijom u uzorcima limfnih čvorova kod još 3 grla, ali izolacija nije uspela usled kontaminacije. Na osnovu klasičnih bakterioloških metoda, izgleda i rasta kolonija, kao i primenom standardnih biohemijskih testova identifikacije, svi izolati su tipizovani u vrstu M. bovis. Na osetljivost prema izoniazidu, streptomicinu, etambutolu i rifampicinu, antituberkuloticima prve linije koji se u našoj zemlji koriste za lečenje ljudi obolelih od tuberkuloze, standardnom metodom proporcija, ispitano je 5 reprezentativnih izolata. Izolati su bili poreklom od goveda iz pet različitih zapata lociranih u naseljima na teritoriji 5 opština, od kojih su 4 u Južnobačkom i 1 u Sremskom okrugu. Ustanovljena je osetljivost izolata na sve ispitivane antituberkulotike, odnosno ni u jednom slučaju nije utvrđena pojava rezistencije. Metodom PCR spoligotipizacije u cilju molekularne karakterizacije i genotipizacije bakterija M.tuberculosis kompleksa ispitano je 18 izolata mikobakterija kod kojih je proces ekstrakcije DNK bio uspešan. Rezultati molekularne karakterizacije odnosno spoligotipizacije izolovanih mikobakterija pokazuju odsustvo spejsera 1 i niza spejsera od 3 sve do 16, kao i spejsera 28 što je karakteristično za Mycobacterium caprae...The aim of this research was isolation, genotyping and molecular characterization of the causative agent of cattle tuberculosis in the province of Vojvodina, as well as evaluation of susceptibility for isolated zoonotic MTB-complex bacteria towards a panel of selected anti-tuberculosis drugs used in tuberculosis treatment for humans in our country. Additionally, the goal of the research was to estimate sensitivity of γ-IFN test for tuberculosis diagnosis in cattle compared to standard microbiological methods of the isolation or histopathological identification of lesions. Finally, based on the animal incidence and herd prevalence per year, the objective was to create the actual epizootiological map of disease occurrence in Vojvodina province. For the isolation of MTB-complex bacteria tissue samples (lung and lymph nodes) from 49 test positive cattle on single comparative tuberculin test were used. During the post mortem examination at the abattoir, visible lesions were present in 40 animals. MTB-complex bacteria were successfully isolated from the pathological material of 19 bovines, while direct microscopic examination of lymph node smears yielded positive result for acid fast bacteria, in additional three animals, but the isolation failed due to contamination or other reasons. Based on the classic bacteriological methods, growth characteristics, colony morphology and biochemical tests all isolates were designated as M. bovis species. PCR-based spoligotyping, was performed on 18 MTB-complex isolates following successful DNA extraction, and a single spoligotype pattern was identified. The results of the molecular characterization, revealed the absence of spacer 1, spacer region 3-16, as well as spacer 28, in all isolates, a characteristic pattern for Mycobacterium caprae. The absence of spacer 28 and spacer region 39-43 suggests that all isolates belong to single cluster. Automated analysis of the binary code of the submitted spoligotype patterns in two largest online databases came out with identifier number SB 0418 and ST 647 respectively, which represents the predominant Mycobacterium caprae cluster in Central-European countries..

Dermatomycosis: A potential source of zoonotic infection in cities

Skin infections in animals and humans represent a complex syndrome that is, from etiological point of view, often hard to solve. Skin is one of the largest organs and plays an important role in the organism of mammals. Depending on the age, it presents 5-8% of total body mass in humans, 12% of adult animals, and even up to 24% in newly born animals. The population of pets and stray dogs in cities provides a possibility for contacts between animals and/or humans, which is a mode of transmission for some diseases. Fungal diseases in dogs caused by dermatophyte are zoonosis, contagious infections that affect dogs and cats, but also other animals, as well as humans. There has been an overall increase in the number of the cases caused by anthropophilic fungi from the strain of Microsporum spp. and Trichophyton spp. Our research was related to animals with different changes on skin manifested by hairless surfaces, dandruff, red skin and extensive pruritus. The objective of our examination was the analyses of scarified samples and swob from canine skin with the aim to determine whether the changes were caused by dermatophytosis. Our aim was to point out the possibility of transmission of mycotic infections from animals to humans in households. For this mycological examination swobs and skin scarifications from dogs, were used. The samples were streaked on Sabo and dextrose agar and incubated at 25°C for at least 21 days. In one year period 81 samples were collected out of which 11 (13.58%) were positive for dermatophyte. In all samples Microsporum canis was isolated. The findings of Microsporum canis in 13.58% of the examined samples indicate the importance of mycological skin disorder in pets. Having in mind that this is a zoonosis, the findings point to a possibility of exposing the humans to fungal infection, affecting almost all pet owners, especially in urban environment

Uticaj različitih mikotoksina na zdravlje svinja - zapažanja s terena

Mycotoxins are structurally diverse fungal metabolites that can contaminate a variety of dietary components consumed by animals and humans. The aim of this paper was to present the field observations of clinical and pathological consequences on swine health in the cases when Fusarium mycotoxins were detected in swine feed. The material for research included the samples from swine farms located in the region of Vojvodina, where health disorders resembling intestinal problems in different swine categories were detected. The applied research methods included: epidemiological and clinical evaluation, gross pathology examination, bacteriological tissue testing originating from diseased dead animals. The presence of deoxynivalenol (DON), T-2 toxin and zearalenone (ZEA) in thirteen complete swine feed mixtures were analyzed by enzyme-linked immunosorbent assay methods, using Ridascreen®FAST DON, Ridascreen®FAST T2, and Ridascreen®FAST Zearalenon test kits (R-Biopharm, Germany). By clinical and pathological examination, the lesions predominantly located in digestive tract were observed in different swine categories. The problem of persistent enteric infections in suckling piglets and alteration of growth performance were notified in weaners and fatteners. In adult categories, reduced feed consumption, sometimes distinct feed refusal and vomiting were observed. In all examined samples of complete feed mixtures for different swine categories the concentration of DON exceeded the maximum permitted levels, but also the presence of other Fusarium mycotoxins was detected. The obtained results indicate the existence of feed mixtures contamination with low levels of Fusarium mycotoxins and their possible positive interaction with etiological agents present in swine farms.Mikotoksini su strukturno različiti metaboliti plesni koji mogu da kontaminiraju komponente koje se koriste u ishrani životinja i ljudi. Cilj rada bio je da se prikažu terenska zapažanja kliničkih i patoloških posledica po zdravlje svinja u slučajevima kada su Fusarium mikotoksini utvrđeni u hrani za ovu vrstu. Materijal za ispitivanje je obuhvatao uzorke poreklom s farmi svinja u Vojvodini, na kojima su registrovani zdravstveni problemi intestinalnog trakta kod različitih kategorija. Primenjene metode ispitivanja su obuhvatale: epizootiološka i klinička ispitivanja, makropatomorfološki pregled uginulih jedinki, bakteriološko i molekularno ispitivanje uzoraka organa i tkiva poreklom od obolelih, uginulih jedinki i mikrobiološko ispitivanje hrane u cilju kontrole prisustva plesni. Prisustvo deoksinivalenola, T-2 toksina i zearalenona je ispitivano u 14 uzoraka različitih kompletnih smeša za svinje primenom imunoenzimske tehnike (Ridascreen®FAST DON, Rida­ screen®FAST T2, Ridascreen®FAST Zearalenon, R-Biopharm, Germany). Kliničkim i patološkim pregledom, kod različitih kategorija svinja, utvrđene su lezije dominantno u digestivnom traktu. Problem upornih enteralnih infekcija kod prasadi na sisi i promene u porastu su zabeležene kod zalučene prasadi i tovljenika. Kod odraslih kategorija svinja, utvrđena je umanjena konzumacija hrane, ponekad izraženo odbijanje hrane i povraćanje. U svim ispitanim uzorcima kompletnih smeša za različite kategorije svinja koncentracija mikotoksina DON bila je veća od maksimalno dozvoljenih vrednosti. U ispitanim uzorcima hrane utvrđeno je i prisustvo drugih Fusarium mikotoksina. Postignuti rezultati ukazuju na kontaminaciju hraniva s niskim vrednostima Fusarium mikotoksina i na njihovu moguću pozitivnu interreakciju s uzročnicima bolesti prisutnim na farmama svinja

Isolation, identification and molecular characterization of causative agent of bovine tuberculosis in Vojvodina region

Cilј istraživanja bio je da se izvrši izolacija, identifikacija i molekularna karakterizacija, uzročnika tuberkuloze goveda u Vojvodini, kao i ispitivanje osetlјivosti izolovanih sojeva na odabrane antituberkulozne antibiotike koji se koriste za terapiju tuberkuloze lјudi u našoj zemlјi. Pored toga cilj istraživanja bilo je i uporedno ispitivanje osetlјivosti i specifičnosti γ-IFN testa u dijagnostici tuberkuloze sa klasičnim mikrobiološkim metodama izolacije i identifikacije uzročnika. Konačno, na osnovu ispitivanja raširenosti, incidencije i prevalencije oboljenja u zapatima cilj je bila i izrada aktuelne epizootiološke karte raširenosti tuberkuloze goveda u Vojvodini. Za izolaciju bakterija MTB-kompleksa korišćeni su uzorci tkiva (pluća i limfni čvorovi) 49 goveda koja su prilikom izvođenja komparativnog tuberkulinskog ili γ-IFN testa imala pozitivnu reakciju. Kod 40 od ovih grla prilikom patomorfološkog pregleda na liniji klanja, bile su ustanovljene promene koje mogu ukazivati na tuberkulozu. Bakterije MTB-kompleksa uspešno su izolovane iz uzoraka tkiva 19 goveda, dok je nalaz acidorezistentnih bakterija ustanovljen direktnom mikroskopijom u uzorcima limfnih čvorova kod još 3 grla, ali izolacija nije uspela usled kontaminacije. Na osnovu klasičnih bakterioloških metoda, izgleda i rasta kolonija, kao i primenom standardnih biohemijskih testova identifikacije, svi izolati su tipizovani u vrstu M. bovis. Na osetljivost prema izoniazidu, streptomicinu, etambutolu i rifampicinu, antituberkuloticima prve linije koji se u našoj zemlji koriste za lečenje ljudi obolelih od tuberkuloze, standardnom metodom proporcija, ispitano je 5 reprezentativnih izolata. Izolati su bili poreklom od goveda iz pet različitih zapata lociranih u naseljima na teritoriji 5 opština, od kojih su 4 u Južnobačkom i 1 u Sremskom okrugu. Ustanovljena je osetljivost izolata na sve ispitivane antituberkulotike, odnosno ni u jednom slučaju nije utvrđena pojava rezistencije. Metodom PCR spoligotipizacije u cilju molekularne karakterizacije i genotipizacije bakterija M.tuberculosis kompleksa ispitano je 18 izolata mikobakterija kod kojih je proces ekstrakcije DNK bio uspešan. Rezultati molekularne karakterizacije odnosno spoligotipizacije izolovanih mikobakterija pokazuju odsustvo spejsera 1 i niza spejsera od 3 sve do 16, kao i spejsera 28 što je karakteristično za Mycobacterium caprae...The aim of this research was isolation, genotyping and molecular characterization of the causative agent of cattle tuberculosis in the province of Vojvodina, as well as evaluation of susceptibility for isolated zoonotic MTB-complex bacteria towards a panel of selected anti-tuberculosis drugs used in tuberculosis treatment for humans in our country. Additionally, the goal of the research was to estimate sensitivity of γ-IFN test for tuberculosis diagnosis in cattle compared to standard microbiological methods of the isolation or histopathological identification of lesions. Finally, based on the animal incidence and herd prevalence per year, the objective was to create the actual epizootiological map of disease occurrence in Vojvodina province. For the isolation of MTB-complex bacteria tissue samples (lung and lymph nodes) from 49 test positive cattle on single comparative tuberculin test were used. During the post mortem examination at the abattoir, visible lesions were present in 40 animals. MTB-complex bacteria were successfully isolated from the pathological material of 19 bovines, while direct microscopic examination of lymph node smears yielded positive result for acid fast bacteria, in additional three animals, but the isolation failed due to contamination or other reasons. Based on the classic bacteriological methods, growth characteristics, colony morphology and biochemical tests all isolates were designated as M. bovis species. PCR-based spoligotyping, was performed on 18 MTB-complex isolates following successful DNA extraction, and a single spoligotype pattern was identified. The results of the molecular characterization, revealed the absence of spacer 1, spacer region 3-16, as well as spacer 28, in all isolates, a characteristic pattern for Mycobacterium caprae. The absence of spacer 28 and spacer region 39-43 suggests that all isolates belong to single cluster. Automated analysis of the binary code of the submitted spoligotype patterns in two largest online databases came out with identifier number SB 0418 and ST 647 respectively, which represents the predominant Mycobacterium caprae cluster in Central-European countries..

Isolation, identification and molecular characterization of causative agent of bovine tuberculosis in Vojvodina region

Cilј istraživanja bio je da se izvrši izolacija, identifikacija i molekularna karakterizacija, uzročnika tuberkuloze goveda u Vojvodini, kao i ispitivanje osetlјivosti izolovanih sojeva na odabrane antituberkulozne antibiotike koji se koriste za terapiju tuberkuloze lјudi u našoj zemlјi. Pored toga cilj istraživanja bilo je i uporedno ispitivanje osetlјivosti i specifičnosti γ-IFN testa u dijagnostici tuberkuloze sa klasičnim mikrobiološkim metodama izolacije i identifikacije uzročnika. Konačno, na osnovu ispitivanja raširenosti, incidencije i prevalencije oboljenja u zapatima cilj je bila i izrada aktuelne epizootiološke karte raširenosti tuberkuloze goveda u Vojvodini. Za izolaciju bakterija MTB-kompleksa korišćeni su uzorci tkiva (pluća i limfni čvorovi) 49 goveda koja su prilikom izvođenja komparativnog tuberkulinskog ili γ-IFN testa imala pozitivnu reakciju. Kod 40 od ovih grla prilikom patomorfološkog pregleda na liniji klanja, bile su ustanovljene promene koje mogu ukazivati na tuberkulozu. Bakterije MTB-kompleksa uspešno su izolovane iz uzoraka tkiva 19 goveda, dok je nalaz acidorezistentnih bakterija ustanovljen direktnom mikroskopijom u uzorcima limfnih čvorova kod još 3 grla, ali izolacija nije uspela usled kontaminacije. Na osnovu klasičnih bakterioloških metoda, izgleda i rasta kolonija, kao i primenom standardnih biohemijskih testova identifikacije, svi izolati su tipizovani u vrstu M. bovis. Na osetljivost prema izoniazidu, streptomicinu, etambutolu i rifampicinu, antituberkuloticima prve linije koji se u našoj zemlji koriste za lečenje ljudi obolelih od tuberkuloze, standardnom metodom proporcija, ispitano je 5 reprezentativnih izolata. Izolati su bili poreklom od goveda iz pet različitih zapata lociranih u naseljima na teritoriji 5 opština, od kojih su 4 u Južnobačkom i 1 u Sremskom okrugu. Ustanovljena je osetljivost izolata na sve ispitivane antituberkulotike, odnosno ni u jednom slučaju nije utvrđena pojava rezistencije. Metodom PCR spoligotipizacije u cilju molekularne karakterizacije i genotipizacije bakterija M.tuberculosis kompleksa ispitano je 18 izolata mikobakterija kod kojih je proces ekstrakcije DNK bio uspešan. Rezultati molekularne karakterizacije odnosno spoligotipizacije izolovanih mikobakterija pokazuju odsustvo spejsera 1 i niza spejsera od 3 sve do 16, kao i spejsera 28 što je karakteristično za Mycobacterium caprae...The aim of this research was isolation, genotyping and molecular characterization of the causative agent of cattle tuberculosis in the province of Vojvodina, as well as evaluation of susceptibility for isolated zoonotic MTB-complex bacteria towards a panel of selected anti-tuberculosis drugs used in tuberculosis treatment for humans in our country. Additionally, the goal of the research was to estimate sensitivity of γ-IFN test for tuberculosis diagnosis in cattle compared to standard microbiological methods of the isolation or histopathological identification of lesions. Finally, based on the animal incidence and herd prevalence per year, the objective was to create the actual epizootiological map of disease occurrence in Vojvodina province. For the isolation of MTB-complex bacteria tissue samples (lung and lymph nodes) from 49 test positive cattle on single comparative tuberculin test were used. During the post mortem examination at the abattoir, visible lesions were present in 40 animals. MTB-complex bacteria were successfully isolated from the pathological material of 19 bovines, while direct microscopic examination of lymph node smears yielded positive result for acid fast bacteria, in additional three animals, but the isolation failed due to contamination or other reasons. Based on the classic bacteriological methods, growth characteristics, colony morphology and biochemical tests all isolates were designated as M. bovis species. PCR-based spoligotyping, was performed on 18 MTB-complex isolates following successful DNA extraction, and a single spoligotype pattern was identified. The results of the molecular characterization, revealed the absence of spacer 1, spacer region 3-16, as well as spacer 28, in all isolates, a characteristic pattern for Mycobacterium caprae. The absence of spacer 28 and spacer region 39-43 suggests that all isolates belong to single cluster. Automated analysis of the binary code of the submitted spoligotype patterns in two largest online databases came out with identifier number SB 0418 and ST 647 respectively, which represents the predominant Mycobacterium caprae cluster in Central-European countries..

Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja

The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05).Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05)

Dermatomycosis: Conditions that contribute to the disease development

Skin lesions caused by dermatophytes are classified depending on the infected skin structure: surface layer of cutis, fur layer, clutches or nails. Surface mycoses are caused by dermatophytes: Epidermophyton, Microsporum and Trichophyton species (also important are Malassezia spp., Candida spp., and Trichosporon). Skin is the target tissue for fungal infections if the epithelial layer is damaged and immune system cannot cope with the infection, or if the conditions are favorable for dermatophytes, which spread in the cutis due to the enzyme activities. Dermatophytes can be found on skin surface if they contaminate or colonize epidermis or hair follicles. However, clinical symptoms of lesion on the skin are sometimes absent. According to the literature data 6-9% of skin lesions are caused by dermatophyte in human medicine. Similar situation is in veterinary medicine. Fungus that cause dermatomycosis are widespread in the nature and could be divided into: zoophilic, geophilic and anthrophilic. The goal of this paper is to present the latest knowledge in pathogenesis on dermatomycosis, predisposing factors important for the outcome of the disease, and immunological reaction of organism to the fungal infection. Our intention is to summarize the subject and present the facts related to specific problems in dermatomycosis

Impact of fusarium mycotoxins on swine health - field observations

Mycotoxins are structurally diverse fungal metabolites that can contaminate a variety of dietary components consumed by animals and humans. The aim of this paper was to present the field observations of clinical and pathological consequences on swine health in the cases when Fusarium mycotoxins were detected in swine feed. The material for research included the samples from swine farms located in the region of Vojvodina, where health disorders resembling intestinal problems in different swine categories were detected. The applied research methods included: epidemiological and clinical evaluation, gross pathology examination, bacteriological tissue testing originating from diseased dead animals. The presence of deoxynivalenol (DON), T-2 toxin and zearalenone (ZEA) in thirteen complete swine feed mixtures were analyzed by enzyme-linked immunosorbent assay methods, using Ridascreen®FAST DON, Ridascreen®FAST T2, and Ridascreen®FAST Zearalenon test kits (R-Biopharm, Germany). By clinical and pathological examination, the lesions predominantly located in digestive tract were observed in different swine categories. The problem of persistent enteric infections in suckling piglets and alteration of growth performance were notified in weaners and fatteners. In adult categories, reduced feed consumption, sometimes distinct feed refusal and vomiting were observed. In all examined samples of complete feed mixtures for different swine categories the concentration of DON exceeded the maximum permitted levels, but also the presence of other Fusarium mycotoxins was detected. The obtained results indicate the existence of feed mixtures contamination with low levels of Fusarium mycotoxins and their possible positive interaction with etiological agents present in swine farms. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR31071

Farm water as a possible source of fungal infections

The quality of drinking water depends on the water sources, but also on the quality of the water distribution system which supplies the water on to the final user. In addition, the possibility of contamination of water used for watering animals in the farm buildings depends on the hygienic conditions on farms. Microbiological quality of water on farms in Serbia has not been one of the main focuses of animal breeders, although according to the Food Safety Law water is considered as food. As feed safety for the animals, which includes microbiological analyses, is an important concern of breeder farmers, it is also important to control the water safety in order not to become a cause of the animal health problems. Change of the water quality is not important only from the sanitary epidemiological point of view, but the presence of different microorganisms, especially fungi, can cause changes in taste and smell, as organoleptic properties of water. According to legal regulations, there is no difference between the quality requirements for drinking water relative to the water supply intended for animals. For the aforementioned reasons, the subject of this study is microbiological control of water samples from the drinkers for animals at farms. The aim of the work is to examine which fungi are possibly present in the water and what their number is. In total, 35 samples of water from pig and poultry farms were tested. The method of direct seeding and filtration was used. The presence of different types of mold (Aspergillus sp., Penicillium sp., Alternaria sp., Mucor sp. and Rhizopus sp., and Candida sp.) was determined. The results indicate the necessity of microbiological control of water for watering of farm animals, which implies the analysis for the presence of molds. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR31071