9 research outputs found
Isothermal titration calorimetry as a complementary method for investigating nanoparticle-protein interactions
Controlling protein interactions in blood for effective liver immunosuppressive therapy by silica nanocapsules
Immunosuppression with glucocorticoids is a common treatment for autoimmune liver diseases and after liver transplant, which is however associated with severe side-effects. Targeted delivery of glucocorticoids to inflammatory cells, e.g. liver macrophages and Kupffer cells, is a promising approach for minimizing side effects. Herein, we prepare coreâshell silica nanocapsules (SiO2 NCs) via a solâgel process confined in nanodroplets for targeted delivery of dexamethasone (DXM) for liver immunosuppressive therapy. DXM with concentrations up to 100 mg mLâ1 in olive oil are encapsulated while encapsulation efficiency remains over 95% after 15 days. Internalization of NCs by non-parenchymal murine liver cells significantly reduces the release of inflammatory cytokines, indicating an effective suppression of inflammatory response of liver macrophages. Fluorescent and magnetic labeling of the NCs allows for monitoring their intracellular trafficking and biodegradation. Controlled interaction with blood proteins and good colloidal stability in blood plasma are achieved via PEGylation of the NCs. Specific proteins responsible for stealth effect, such as apolipoprotein A-I, apolipoprotein A-IV, and clusterin, are present in large amounts on the PEGylated NCs. In vivo biodistribution investigations prove an efficient accumulation of NCs in the liver, underlining the suitability of the SiO2 NCs as a dexamethasone carrier for treating inflammatory liver diseases.Fil: Jiang, Shuai. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Prozeller, Domenik. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Pereira, Jorge. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Simon, Johanna. Max-Planck-Institut fĂŒr Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Han, Shen. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Wirsching, Sebastian. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Fichter, Michael. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Mottola, Milagro. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - CĂłrdoba. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas. Universidad Nacional de CĂłrdoba. Facultad de Ciencias Exactas, FĂsicas y Naturales. Instituto de Investigaciones BiolĂłgicas y TecnolĂłgicas; Argentina. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Lieberwirth, Ingo. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: Morsbach, Svenja. Max-Planck-Institut fĂŒr Polymerforschung; AlemaniaFil: MailĂ€nder, Volker. Max-Planck-Institut fĂŒr Polymerforschung; Alemania. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Gehring, Stephan. Johannes Gutenberg Universitat Mainz; AlemaniaFil: Crespy, Daniel. Max-Planck-Institut fĂŒr Polymerforschung; Alemania. Vidyasirimedhi Institute of Science and Technology; TailandiaFil: Landfester, Katharina. Max-Planck-Institut fĂŒr Polymerforschung; Alemani
Coating nanoparticles with tunable surfactants facilitates control over the protein corona
Probing the Fluorescence Behavior of DNAâStabilized Silver Nanoclusters in the Presence of Biomolecules
Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020
The last 5 years have seen a series of advances in the application of isothermal titration microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in understanding multiprotein complex formation including proteolysis-targeting chimeras (PROTACS), and mitochondrial autophagy receptor Nix interaction with LC3 and GABARAP. It has also helped elucidate complex allosteric communication in protein complexes like trp RNA-binding attenuation protein (TRAP) complex. Advances in kinetics analysis have enabled the calculation of kinetic rate constants from pre-existing ITC data sets. Diverse strategies have also been developed to study enzyme kinetics and enzyme-inhibitor interactions. ITC has also been applied to study small molecule solvent and solute interactions involved in extraction, separation, and purification applications including liquid-liquid separation and extractive distillation. Diverse applications of ITC have been developed from the analysis of protein instability at different temperatures, determination of enzyme kinetics in suspensions of living cells to the adsorption of uremic toxins from aqueous streams