203 research outputs found
Soft Listeria: actin-based propulsion of liquid drops
We study the motion of oil drops propelled by actin polymerization in cell
extracts. Drops deform and acquire a pear-like shape under the action of the
elastic stresses exerted by the actin comet. We solve this free boundary
problem and calculate the drop shape taking into account the elasticity of the
actin gel and the variation of the polymerization velocity with normal stress.
The pressure balance on the liquid drop imposes a zero propulsive force if
gradients in surface tension or internal pressure are not taken into account.
Quantitative parameters of actin polymerization are obtained by fitting theory
to experiment.Comment: 5 pages, 4 figure
Coordination of Kinesin Motors Pulling on Fluid Membranes
AbstractIntracellular transport relies on the action of motor proteins, which work collectively to either carry small vesicles or pull membranes tubes along cytoskeletal filaments. Although the individual properties of kinesin-1 motors have been extensively studied, little is known on how several motors coordinate their action and spatially organize on the microtubule when pulling on fluid membranes. Here we address these questions by studying, both experimentally and numerically, the growth of membrane tubes pulled by molecular motors. Our in vitro setup allows us to simultaneously control the parameters monitoring tube growth and measure its characteristics. We perform numerical simulations of membrane tube growth, using the experimentally measured values of all parameters, and analyze the growth properties of the tube considering various motor cooperation schemes. The comparison of the numerical results and the experimental data shows that motors use simultaneously several protofilaments of a microtubule to pull a single tube, as motors moving along a single protofilament cannot generate the forces required for tube extraction. In our experimental conditions, we estimate the average number of motors pulling the tube to be approximately nine, distributed over three contiguous protofilaments. Our results also indicate that the motors pulling the tube do not step synchronously
Stress Clamp Experiments on Multicellular Tumor Spheroids
The precise role of the microenvironment on tumor growth is poorly
understood. Whereas the tumor is in constant competition with the surrounding
tissue, little is known about the mechanics of this interaction. Using a novel
experimental procedure, we study quantitatively the effect of an applied
mechanical stress on the long-term growth of a spheroid cell aggregate. We
observe that a stress of 10 kPa is sufficient to drastically reduce growth by
inhibition of cell proliferation mainly in the core of the spheroid. We compare
the results to a simple numerical model developed to describe the role of
mechanics in cancer progression.Comment: 5 pages, 4 figure
Undulation Instability of Epithelial Tissues
Treating the epithelium as an incompressible fluid adjacent to a viscoelastic
stroma, we find a novel hydrodynamic instability that leads to the formation of
protrusions of the epithelium into the stroma. This instability is a candidate
for epithelial fingering observed in vivo. It occurs for sufficiently large
viscosity, cell-division rate and thickness of the dividing region in the
epithelium. Our work provides physical insight into a potential mechanism by
which interfaces between epithelia and stromas undulate, and potentially by
which tissue dysplasia leads to cancerous invasion.Comment: 4 pages, 3 figure
Mechanical Instabilities of Biological Tubes
We study theoretically the shapes of biological tubes affected by various
pathologies. When epithelial cells grow at an uncontrolled rate, the negative
tension produced by their division provokes a buckling instability. Several
shapes are investigated : varicose, enlarged, sinusoidal or sausage-like, all
of which are found in pathologies of tracheal, renal tubes or arteries. The
final shape depends crucially on the mechanical parameters of the tissues :
Young modulus, wall-to-lumen ratio, homeostatic pressure. We argue that since
tissues must be in quasistatic mechanical equilibrium, abnormal shapes convey
information as to what causes the pathology. We calculate a phase diagram of
tubular instabilities which could be a helpful guide for investigating the
underlying genetic regulation
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