MODE OF ACTION OF STHANIK CHIKITSA IN COMMONLY USED IN STREE ROGA

Yoni Roga do not occur without vitiation of Apana Vata, thus first of all Vata should be normalized then treatment of other Doshas should be done. Sthanika Chikitsa (local therapies) prescribed by ancient Acharyas as Yoni Prakshalana (cleansing of vagina), Yoni Pichu (medication soaked tampon place in the vagina), Yoni Purana (vaginal packing), Yoni Lepa (semisolid drug applied in vaginal wall), Yoni Varti (vaginal suppository), Yoni Dhoopana (vaginal fumigation) & Uttarbasti (medicated oil/Ghrita pushed in the uterine cavity). For better result of this therapy the appropriate knowledge of mode of action of drug ought to be important. Our Acharyas very well know the mode of action of this Sthanika Chikitsa and describe the specific Sthanika Chikista according to different Yoni- Vyapada or vitiated Doshas. The reason behind for chosen the vaginal route because of the rugae of the vaginal epithelium create a invoulted surface and results in a large surface area provide, this large surface area allows the trans-epithelial absorption of medications via the vaginal route & the posterior fornix have rich blood supply so actively absorption of drug. The main objective of this literature to find out the probable mode of action of special drug in specific Sthanik Chikitsa

Ayurvedic Approach to Secondary Infertility

The God has blessed the female with most valuable gift of motherhood mother is also called as “Janani'' who give birth to child. A woman hood is never considered complete without achievement of motherhood. Infertility is a burning issue because today's changing lifestyle and stress increases metabolic and hormonal disturbance as a result diabetes, PCOS, infertility. Our focus is, if a woman having normal anatomy of pelvic organ than she should conceive by natural way by Ayurvedic treatment because more side effect of ovulation induction and hormonal therapy. In Ayurveda Bandhyatva (infertility) mainly occurs vitiation of Vata and Kapha Dosha, in some case Pitta may involve. Fertility also depends on mental condition of couple as Charak says sound mind couple gets conceived easily. In this patient had also metabolic disturbance and stress, and ineffective endometrial receptiveness, so we choose Ashwagandha (Withania somnifera) as a anti-stress and normalize Vata and Kapha and Shatavari (Asparagus racemosa) for endometrial tonicity and Sthanik (endometrial) Pitta Shaman and Chandraprabha for Apana Vata (pelvic) disorder, balancing Tridosha and for Dhatvagni Deepan (better metabolism)

A Study of Kapikachchhu Moola Kwatha for Yoni Prakshalana and Palasha Udumbara Sidhha Tail Pichu In Prasramsini/ Phalini/ Mahayoni w.s.r. to Uterovaginal Prolapse

Pelvic organ prolapse is the downward displacement of structures that are normally located adjacent to the vaginal vault. Aims & Objectives: To study the effect of Kapikachchhu Moola Kwatha for Yoni Prakshalana (vaginal douching by Mucuna prurience) and Palasha, Udumbara Siddha Tail Pichu (vaginal tampon by oil prepared with Butea monosperma and Ficus glomerata) in Prasramsini, Phalini & Maha Yoni (Uterovaginal prolapse). To evaluate the comparative effect of Kapikachchhu Moola Kwatha Yoni Prakshalana and Palasha Udumbara Tail Yoni Pichu. Materials & Methods: Randomised control trial was conducted on 37 patients and divided in three groups, 13 patients in group A and 12-12 patients in group B and C registered. In group A, administered Palasha Udumbara Tail Yoni Pichu. In group B, administered Kapikachchhu Moola Kwatha Yoni Prakshalana. In Group C, administered combined therapy. Observations and Results: Different parameters like something coming out per vaginum, Yoni Srava (white discharge per vaginum), Yoni Kandu (itching per vaginum), Yoni Vedna (dragging pain in vagina), Parva Vankshana Shoola (Midsacral discomfort), increase frequency of micturition, stress urinary incontinence, difficulty in emptying bladder, frequency of bowel movements, straining during defecation and objective parameters assessed by POP-Q test that is; Aa and Ba on anterior compartment, C and D on middle compartment, point Ap and Bp on posterior compartment were observed before, during and after the treatment. The overall effect of therapy, in group A; 60% patients got mild relief (25.1% to 50% relief), followed by 30% got moderate relief (50.1% to 75% relief). In group B; 80% patients got mild relief. In group C; 50% patients got moderate relief and 50% got mild improvement. Conclusion: Combined therapy is more effective than single therapy. In single therapy, Group A is better than Group B. Maximum patients of Phalini and Prasramsini Yoni (2nd degree uterovaginal descent) got moderate improvement and Mahayoni (3rd degree uterovaginal descent) patients got mild improvement

Multi-Knock—a multi-targeted genome-scale CRISPR toolbox to overcome functional redundancy in plants

International audiencePlant genomes are characterized by large and complex gene families that often result in similar and partially overlapping functions 1. This genetic redundancy severely hampers current efforts to uncover novel phenotypes, delaying basic genetic research and breeding programs 2. Here, we describe the development and validation of Multi-Knock, a genome-scale CRISPR toolbox that overcomes functional redundancy in Arabidopsis by simultaneously targeting multiple gene-family members, thus identifying genetically hidden components. We computationally designed 59,129 optimal single guide RNAs (sgRNAs) that each target 2 to 10 genes within a family at once. Furthermore, partitioning the library into ten sub-libraries directed towards a different functional group allows flexible and targeted genetic screens. From the 5,635 sgRNAs targeting the plant transportome, we generated over 3,500 independent Arabidopsis lines that allowed us to identify and characterize the first known cytokinin tonoplast-localized transporters in plants. With the ability to overcome functional redundancy in plants at the genome-scale level, the developed strategy can be readily deployed by scientists and breeders for basic research and to expedite breeding efforts