Cytomorphometric Analysis of Normal Exfoliative Cells from Buccal Mucosa in Different Age Groups

Oral exfoliative cytology is a simple and relatively pain free procedure which can be carried out repeatedly with minimum discomfort to the patients. Oral exfoliative cytology has sentivity of 89% and specificity of 89.5%, its accuracy could be enhanced by cytomorphometric analysis of the cells. On reviewing the literature we found numerous articles on cytomorphometric analysis of premalignant and malignant lesions and tobacco chewers-related lesions, but the articles available for the analysis of the normal epithelial cells are relatively few in number. The present study was carried out to assess the cytoplasmic diameter and nuclear diameters of normal buccal mucous membrane under various age groups.Materials and methods:The patients were divided into 3 groups, group-I consists of patients less than 25 years of age, Group – II consists of patients of 25 -50 years of age and Group –III of patients more than 50 years of age. Scrapings were made from the buccal mucosa and immediately fixed with 95% ethanol for a minimum of 15 minutes. In all the cases the Nuclear diameter (ND) and the Cell diameter (CD) were measured in both the horizontal and vertical axes in micrometer. The Nuclear Cytoplasmic(NC) Ratio was also calculated for each case.Statistical analysis:For each age group the mean ND, mean CD, and mean NC ratio were determined. One sample‘t’ test was carried out to find the significance of each sample.Results:The cytomorphometric analysis of the exfoliated normal buccal mucosal cells revealed that the ND varied from 7.4μ to 8.4μ, the cytoplasmic diameter from 46.77μ to 49.34μ and the nuclear cytoplasmic ratio from 0.17μ to 0.20μ. The increase in ND and decrease in CD are due to aging process and decreased cell metabolic activity respectively

Spontaneous heterotopic pregnancy: a rare possibility

Heterotopic pregnancy is defined as a condition when intrauterine and extrauterine pregnancy occur simultaneously. It is a life-threatening condition that requires immediate and accurate diagnostics and treatment. We present the case of a 34-year-old multigravida in her 7 weeks of gestation came to emergency ward with complains of pain in abdomen for 3 days. Ultrasonographic examination showed a live intrauterine pregnancy and mild to moderate free fluid in abdomen with moving internal echoes. An emergency exploratory laparotomy done and left sided tubal ectopic removed along with right salpingectomy as patient was willing for family planning. A high index of suspicion is required to diagnose Heterotopic pregnancy as clinical as well as radiological findings may be insufficient for diagnosis

Low-Temperature Spin Dynamics of Doped Manganites: roles of Mn-t2g and eg and O-2p states

The low-temperature spin dynamics of doped manganites have been analyzed within a tight-binding model, the parameters of which are estimated by mapping the results of ab initio density functional calculations onto the model. This approach is found to provide a good description of the spin dynamics of the doped manganites, observed earlier within the ab initio calculations. Our analysis not only provides some insight into the roles of the eg and the t2g states but also indicates that the oxygen p states play an important role in the spin dynamics. This may cast doubt on the adaptability of the conventional model Hamiltonian approaches to the analysis of spin dynamics of doped manganites.Comment: 12 pages; Includes 5 figure

Effect of Piperine on an Nrf2/Keap 1 Signalling Mechanism in Adipose Tissue of High Fat Diet and Sucrose-Induced Experimental Diabetic Rats

Piperine, an alkaloid compound found in black pepper has been shown to have various health benefits such as anti-oxidants, anti-inflammatory, and anticancer activities. But mechanisms underlying the anti-diabetic activity are unclear. Keap1-Nrf2 is an anti-oxidant stress signal pathway and it is considered to be an intracellular defence mechanism to countered oxidative stress. The study was aimed at assessing antidiabetic activity of piperine against high fat diet and sucrose-induced (HFD) type-2 diabetic rats by regulating the expression of Nrf2/Keap 1 signalling. Healthy adult male albino rats of wistar strain were grouped in to 5. Considering healthy control (group-1), HFD-induced type-2 diabetes (group2), Diabetic rats treated with piperine (group 3), diabetic rats metformin (group 4) and control +Piperine treated rats ( group 5) respectively. After 30 days of treatment, fasting blood glucose (FBG) checked and adipose tissue from control and treated groups was used to determine the role of piperine on the expression of NrF2/Keap 1 mNRA in adipose by Real Time-PCR analysis. Data were analysed by one-way ANOVA and p<0.01 was considered to be statistical significance among the groups. HFD-induced T2DM showed a significant increase in the levels of FBG and altered levels of Nrf-2 and Keap-1 gene expression (2 fold) compared to normal control animals. Piperine at a dose of 40mg, fascinatingly improved the glycemic control and normalised the mRNA expression of both Nrf-2 and Keap-1 whose effects were near to that of standard drug metformin level (p<0.05) proving its potential mechanism of action. Conclusion: Our current study clearly indicates that piperine controls hyperglycemia in type-2 diabetic rats by facilitating the expression of antioxidant signalling (Nrf-2/Keap-1) in the adipose. In addition, this is the first of its kind to show the role of piperine in reducing hyperglycemia against high fat diet and sucrose –induced type-2 diabetic rats as an invivo experimental model. Hence, piperine could be considered as an important health supplement and potential drug candidate for the treatment of diabetes

Functional outcome in displaced proximal humeral fractures in adults treated by proximal humeral locking plates

Background: The management of proximal humerus fractures (PHF) is a challenging task to any surgeon. Study was conducted to evaluate the clinical and functional outcome of the proximal humeral internal locking system in fixation of displaced proximal humeral fractures.Methods: Study was conducted in the Department of Orthopedics, GSL Medical College. Informed written consent was taken from the study participants. All skeletally mature patients aged >18 years, presenting with displaced PHF according to Neer two, three and four part fracture were included in the study. Either deltopectoral or deltoid splitting approach was used for surgery, post-operative rehabilitation was started on day one.Results: Twenty-five patients with PHF were enrolled in the study; five-holed proximal humerus locking plate (PHLP) was used for 18 patients, eight-holed PHLP for 05 and three-holed, ten-holed PHLP for 01 for one each. The Constant-Murley score was significantly improved (p=0.000) over each successive follow-up period with the average improvement of around 19 scores between 1st and 2nd follow-up and around 15 score improvement between 2nd and 3rd follow-up.Conclusions: The proximal humeral locking plate is an adequate device for the fixation of displaced two-part, three-part and four-part PHF. Patient can regain good shoulder function, resume normal activities much earlier

Primary Ovarian Insufficiency: Current Understanding and Diagnostic Approaches

Primary ovarian insufficiency (POI) is a medical condition where ovarian function stops prematurely, typically before the age of 40. This condition leads to infertility and produces symptoms similar to those experienced during menopause. Although the origins of POIs are diverse, genetic elements substantially influence their emergence. This assessment delves into the genetic facets of POI, covering genetic triggers, detection, and genetic consultation. We scrutinize the genes linked to POI and their function in ovarian activity, as well as the genetic deviations and mutations that foster POI onset. We also examine the challenges and limitations of genetic testing and counseling for POI and suggest ways to address these challenges. This review offers a thorough examination of the existing understanding of the genetic factors linked to Primary Ovarian Insufficiency (POI) emphasizing the critical need for further investigation in this field

Impact of perioperative period on disease-free survival among carcinoma ovary patients treated with the interval cyto-reductive surgery at a tertiary cancer centre in Kerala, India: a retrospective study

Background: Global incidence of ovarian malignancies is 300,000 as per GLOBOCAN 2018. The treatment protocol for advanced ovarian malignancies (stage IIIc and stage IV) includes neo-adjuvant chemotherapy and surgery followed by adjuvant chemotherapy. Aims of the study was to determine the effect of duration of chemo interruption on disease free survival of ovarian malignancies treated by interval cytoreduction followed by surgery.Methods: A total 48 patients were studied for events such as recurrence, death, patient’s status on last follow up, peri-operative period between 3rd cycle of chemo therapy and 4th cycle of chemo therapy. Based on the median duration of peri operative period patients was classified as early or delayed receivers of adjuvant chemo therapy. Difference in duration of over-all survival and disease-free survival was analysed through Kaplan Meier survival analysis using log-rank test. Hazard ratio adjusted for background characteristics such as staging, performance status, grade of tumour were analysed using cox proportional hazard model.Results: The two peri operative period categories based on mean value (85 days) didn’t show any significant association to disease free interval (minimum-21days, maximum-146 days, Hr = 1.3, p-value = 0.52). Other established factors like stage, extent of resection, response to chemotherapy, also didn’t show any significant association. Serum marker level showed a significant negative correlation with disease free survival (minimum-9 days, maximum-30659, p-value =.04, Hr = 3.19).Conclusions: The study could not establish any correlation between peri operative period and median disease-free survival. The small sample size is a limiting factor, well controlled randomized trials may needed for further clarification

Solitary Bone Plasmacytoma of the maxilla- A rare case report

Abstract:Aims and Objectives: Plasmacytoma is a discrete, uni-focal, monoclonal neoplastic proliferation of plasma cells. It can be either solitary or multiple and skeletal or extraskeletal. Solitary bone plasmacytoma accounts for 3% of all plasma cell neoplasms with 50% of cases transforming into multiple myeloma. Since plasmacytoma is a rare lesion in the oral cavity, this case presentation is an attempt to focus on the clinical, histopathological aspects of this lesion and the spectrum of plasma cell neoplasms.Case description: We report a case of solitary bone plasmacytoma in a young patient, presenting as a painless ulcero-proliferative growth in the maxillary region. OPG showed radiolucency with respect to non-healing extracted tooth socket (16) with slight bony erosion. Microscopy revealed mature and immature plasma cells with eccentrically placed nucleus, suggestive of solitary plasmacytoma.Conclusion: The purpose of this article is to report a rare case of solitary bone plasmacytoma with emphasis on diagnostic workup.&nbsp

Virtual screening of the inhibitors targeting at the viral protein 40 of Ebola virus

Multilingual abstracts in the six official working languages of the United Nations. (PDF 373 kb