66 research outputs found
A mouse model that is immunologically tolerant to reporter and modifier proteins
Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8(+) T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains. Bresser and Dijkgraaf et al. develop the 'Tol' strain, a genetically modified mouse model that expresses a range of shuffled reporter and modifier proteins from a single open reading frame. This strain is immunologically tolerant to these reporter and modifier proteins, providing a valuable model system for cell transfer studies and virus-mediated gene transfer studies.Dermatology-oncolog
Contribuição ao conhecimento dos algas do gênero Euglena (Euglenophyceae) no município do Rio de Janeiro e arredores, Brasil
Effect of body colour of Oreochromis mossambicus (Peters) on predation by largemouth bass, Micropterus salmoides (Lacepède)
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