Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil

The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Forty-nine strains (44.9%) exhibited the NTBF pattern III which possesses the flanking region required for pathogenicity island acquisition in which the bftgene is codified. These data reinforce the potential of B. fragilis as an emerging enteropathogen in our country

Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-07-17T17:30:30Z No. of bitstreams: 1 ilanat_balassiano_etal_IOC_2008.pdf: 315376 bytes, checksum: e702ff045eb09dfe8cbf523d59202094 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-07-17T17:52:59Z (GMT) No. of bitstreams: 1 ilanat_balassiano_etal_IOC_2008.pdf: 315376 bytes, checksum: e702ff045eb09dfe8cbf523d59202094 (MD5)Made available in DSpace on 2018-07-17T17:52:59Z (GMT). No. of bitstreams: 1 ilanat_balassiano_etal_IOC_2008.pdf: 315376 bytes, checksum: e702ff045eb09dfe8cbf523d59202094 (MD5) Previous issue date: 2008Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instittuto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Farmácia. Departamento de Tecnologia Farmacêutica. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Fortynine strains (44.9%) exhibited the NTBF pattern III which possesses the flanking region required for pathogenicity island acquisition in which the bft gene is codified. These data reinforce the potential of B. fragilis as an emerging enteropathogen in our country

Desempenho e digestibilidade in vivo de cordeiros alimentados com dietas contendo canola em grão integral em diferentes formas Performance and digestibility in vivo of lambs fed diets with whole canola grain in different forms

O desempenho e a digestibilidade dos nutrientes de cordeiros alimentados com concentrados formulados com farelo de soja (FS), canola integral (CI) canola quebrada (CQ) ou canola peletizada (CP) e feno de aveia, fornecidos na relação 30/70 (volumoso/conconcentrado, %MS) foram avaliados. Vinte oito cordeiros machos com idade inicial entre 60 e 90 dias e 17 kg PV foram distribuídos em delineamento inteiramente casualizado. As ingestões (g/d) de MS, PB, FDA, FDN e EB (Mcal/dia), o ganho médio diário e a conversão alimentar, foram semelhantes. Não houve diferenças para digestibilidade aparente da MS, PB e EB, exceto para digestibilidade de FDN (46,84; 60,11; 50,10; e 38,88%) e FDA (45,84; 54,19; 46,57; e 29,59%) para FS, CI, CQ e CP, respectivamente. Houve menor retenção de nitrogênio para CP (3,0 g/d) em comparação às outras dietas (entre 5,0 e 7,3 g/d). Os tratamentos não diferiram na concentração de propionato, mas reduziram as concentrações de butirato (7,08; 4,87; 4,08; e 4,29 &#956;M/mL de líquido ruminal) e N-amoniacal (12,17; 8,69; 8,40; e 7,66 mg/100 mL de líquido de rúmen). O uso de canola, nas diferentes formas, não influenciou a ingestão e a digestão, proporcionando desempenho semelhante entre os tratamentos.<br>The performance and digestibility of nutrients of lambs fed concentrates formulated with soybean meal (SM) and whole canola grain (WC), cracked canola grain (CC) or pelleted canola (PC) and oat hay, fed in a 30:70 (forage to concentrate ratio, %DM) were evaluated. Twenty-eight male lambs with initial age from 60 to 90 days and 17 kg LW were allotted to a completely randomized design. The intakes (g/d) of DM, CP, ADF, NDF and GE (Mcal/d), the average daily gain and feed: gain ratio, were similar. There were no differences for apparent digestibilities of MS, PB and GE, except for the NDF digestibility (46.84, 60.11, 50.10, and 38.88%) and ADF (45.84, 54.19, 46.57, and 29.59%) for SM, WC, CC and PC, respectively. There was lower nitrogen retention for CP (3.0 g/d) comparing to the other diets (between 5.0 and 7.3 g/d). The treatments did not differ on the propionate concentration, but reduced the concentrations of butyrate (7.08, 4.87, 4.08, and 4.29 &#956;M/mL of ruminal fluid) and ammonia-N (12.17, 8.69, 8.40, and 7.66 mg/100 mL of ruminal fluid). The use of canola, in the different forms, did not affect the intake and digestion, providing similar performance among the treatments

Evolutionary perspectives on bee mtDNA from mito-OMICS analyses of a solitary species

info:eu-repo/semantics/publishe

Diapause in a tropical oil-collecting bee: molecular basis unveiled by RNA-Seq

Abstract Background Diapause is a natural phenomenon characterized by an arrest in development that ensures the survival of organisms under extreme environmental conditions. The process has been well documented in arthropods. However, its molecular basis has been mainly studied in species from temperate zones, leaving a knowledge gap of this phenomenon in tropical species. In the present study, the Neotropical and solitary bee Tetrapedia diversipes was employed as a model for investigating diapause in species from tropical zones. Being a bivoltine insect, Tetrapedia diversipes produce two generations of offspring per year. The first generation, normally born during the wet season, develops faster than individuals from the second generation, born after the dry season. Furthermore, it has been shown that the development of the progeny, of the second generation, is halted at the 5th larval instar, and remains in larval diapause during the dry season. Towards the goal of gaining a better understanding of the diapause phenomenon we compared the global gene expression pattern, in larvae, from both reproductive generations and during diapause. The results demonstrate that there are similarities in the observed gene expression patterns to those already described for temperate climate models, and also identify diapause-related genes that have not been previously reported in the literature. Results The RNA-Seq analysis identified 2275 differentially expressed transcripts, of which 1167 were annotated. Of these genes, during diapause, 352 were upregulated and 815 were downregulated. According to their biological functions, these genes were categorized into the following groups: cellular detoxification, cytoskeleton, cuticle, sterol and lipid metabolism, cell cycle, heat shock proteins, immune response, circadian clock, and epigenetic control. Conclusion Many of the identified genes have already been described as being related to diapause; however, new genes were discovered, for the first time, in this study. Among those, we highlight: Niemann-Pick type C1, NPC2 and Acyl-CoA binding protein homolog (all involved in ecdysteroid synthesis); RhoBTB2 and SASH1 (associated with cell cycle regulation) and Histone acetyltransferase KAT7 (related to epigenetic transcriptional regulation). The results presented here add important findings to the understanding of diapause in tropical species, thus increasing the comprehension of diapause-related molecular mechanisms

Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

Abstract Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands and stroma from the invasive tumor front (ITF) and inner tumor to identify differentially expressed proteins. Potential signature proteins are prioritized and further investigated by immunohistochemistry (IHC) and targeted proteomics. IHC indicates low expression of cystatin-B in neoplastic islands from the ITF as an independent marker for local recurrence. Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. In summary, we identify a robust signature, which may enhance prognostic decisions in OSCC and better guide treatment to reduce tumor recurrence or lymph node metastasis