Examining the Impact of Climate Change Film as an Educational Tool

Purpose: The aim of this paper is to evaluate the effectiveness of film in communicating issues related to climate change. While previous studies demonstrate an immediate effect of a film post-screening, this study also considered if a film can inspire long-term effects, and if supplemental educational information plays a role on participant understanding. Design/methodology/approach: Using surveys, we assessed undergraduate students’ climate change responses pre-, immediately-post, and 9-weeks post watching the climate change documentary The Human Element (Prod. Earth Vision Institute, 2018). In the 9-week interim before the final survey, half of the participants received weekly information on climate change via a custom website, while the other half served as a control. Nonparametric statistical tests were completed in SPSS to determine significant changes across all three surveys. Findings: Friedman tests and Wilcoxon Signed Ranks tests demonstrate statistically significant self-reported impacts on climate change responses such as of motivation, concern, and understanding immediately post-screening. At 9-weeks, 3 × 2 Mixed ANOVAs demonstrate that the group that received the website reported statistically significantly higher understanding than those in the control group. However, the website has no statistically significant effect on other responses like motivation and concern. Originality/value: These results highlight the important power of film’s visual appeals in framing climate change. We also show that there is a long term effect of film on participant understanding. The study also prompts questions about current models of climate change education, which emphasize objective understanding, often without viable support structures to help students’ concern and motivation to act

Incomplete Charge Collection at Inter-Pixel Gap in Low-and High-Flux Cadmium Zinc Telluride Pixel Detectors

The success of cadmium zinc telluride (CZT) detectors in room-temperature spectroscopic X-ray imaging is now widely accepted. The most common CZT detectors are characterized by enhanced-charge transport properties of electrons, with mobility-lifetime products µeτe > 10−2 cm2/V and µhτh > 10−5 cm2/V. These materials, typically termed low-flux LF-CZT, are successfully used for thick electron-sensing detectors and in low-flux conditions. Recently, new CZT materials with hole mobility-lifetime product enhancements (µhτh > 10−4 cm2/V and µeτe > 10−3 cm2/V) have been fabricated for high-flux measurements (high-flux HF-CZT detectors). In this work, we will present the performance and charge-sharing properties of sub-millimeter CZT pixel detectors based on LF-CZT and HF-CZT crystals. Experimental results from the measurement of energy spectra after charge-sharing addition (CSA) and from 2D X-ray mapping highlight the better charge-collection properties of HF-CZT detectors near the inter-pixel gaps. The successful mitigation of the effects of incomplete charge collection after CSA was also performed through original charge-sharing correction techniques. These activities exist in the framework of international collaboration on the development of energy-resolved X-ray scanners for medical applications and non-destructive testing in the food industry

SpliceAid-F : a database of human splicing factors and their RNA-binding sites

A comprehensive knowledge of all the factors involved in splicing, both proteins and RNAs, and of their interaction network is crucial for reaching a better understanding of this process and its functions. A large part of relevant information is buried in the literature or collected in various different databases. By hand-curated screenings of literature and databases, we retrieved experimentally validated data on 71 human RNA-binding splicing regulatory proteins and organized them into a database called 'SpliceAid-F' (http://www.caspur.it/SpliceAidF/). For each splicing factor (SF), the database reports its functional domains, its protein and chemical interactors and its expression data. Furthermore, we collected experimentally validated RNA-SF interactions, including relevant information on the RNA-binding sites, such as the genes where these sites lie, their genomic coordinates, the splicing effects, the experimental procedures used, as well as the corresponding bibliographic references. We also collected information from experiments showing no RNA-SF binding, at least in the assayed conditions. In total, SpliceAid-F contains 4227 interactions, 2590 RNA-binding sites and 1141 'no-binding' sites, including information on cellular contexts and conditions where binding was tested. The data collected in SpliceAid-F can provide significant information to explain an observed splicing pattern as well as the effect of mutations in functional regulatory elements

TRAM (Transcriptome Mapper): database-driven creation and analysis of transcriptome maps from multiple sources

<p>Abstract</p> <p>Background</p> <p>Several tools have been developed to perform global gene expression profile data analysis, to search for specific chromosomal regions whose features meet defined criteria as well as to study neighbouring gene expression. However, most of these tools are tailored for a specific use in a particular context (e.g. they are species-specific, or limited to a particular data format) and they typically accept only gene lists as input.</p> <p>Results</p> <p>TRAM (Transcriptome Mapper) is a new general tool that allows the simple generation and analysis of quantitative transcriptome maps, starting from any source listing gene expression values for a given gene set (e.g. expression microarrays), implemented as a relational database. It includes a parser able to assign univocal and updated gene symbols to gene identifiers from different data sources. Moreover, TRAM is able to perform intra-sample and inter-sample data normalization, including an original variant of quantile normalization (scaled quantile), useful to normalize data from platforms with highly different numbers of investigated genes. When in 'Map' mode, the software generates a quantitative representation of the transcriptome of a sample (or of a pool of samples) and identifies if segments of defined lengths are over/under-expressed compared to the desired threshold. When in 'Cluster' mode, the software searches for a set of over/under-expressed consecutive genes. Statistical significance for all results is calculated with respect to genes localized on the same chromosome or to all genome genes. Transcriptome maps, showing differential expression between two sample groups, relative to two different biological conditions, may be easily generated. We present the results of a biological model test, based on a meta-analysis comparison between a sample pool of human CD34+ hematopoietic progenitor cells and a sample pool of megakaryocytic cells. Biologically relevant chromosomal segments and gene clusters with differential expression during the differentiation toward megakaryocyte were identified.</p> <p>Conclusions</p> <p>TRAM is designed to create, and statistically analyze, quantitative transcriptome maps, based on gene expression data from multiple sources. The release includes FileMaker Pro database management runtime application and it is freely available at <url>http://apollo11.isto.unibo.it/software/</url>, along with preconfigured implementations for mapping of human, mouse and zebrafish transcriptomes.</p

KAONIC HELIUM-4 L-SERIES YIELD MEASUREMENT AT 2.25 g/l DENSITY BY SIDDHARTA-2 at DAΦNE

This article presents the results of the kaonic helium-4 measurement conducted by the SIDDHARTA-2 experiment, aiming to provide crucial insights into the low-energy strong interaction in the strangeness sector. High-precision X-ray spectroscopy is used to examine the interaction between negatively charged kaons and nuclei in atomic systems. The SIDDHARTA-2 setup was optimized through the kaonic helium-4 measurement in preparation for the challenging kaonic deuterium measurement. The kaonic helium-4 measurement at a new density of 2.25 g/l is reported, providing the absolute and relative yields for the L-series transitions, which are essential data for understanding kaonic atom cascade processes

Potentialities of CdZnTe Quasi-Hemispherical Detectors for Hard X-ray Spectroscopy of Kaonic Atoms at the DAΦNE Collider

Kaonic atom X-ray spectroscopy is a consolidated technique for investigations on the physics of strong kaon-nucleus/nucleon interaction. Several experiments have been conducted regarding the measurement of soft X-ray emission (&lt;20 keV) from light kaonic atoms (hydrogen, deuterium, and helium). Currently, there have been new research activities within the framework of the SIDDHARTA-2 experiment and EXCALIBUR proposal focusing on performing precise and accurate measurements of hard X-rays (&gt;20 keV) from intermediate kaonic atoms (carbon, aluminum, and sulfur). In this context, we investigated cadmium-zinc-telluride (CdZnTe or CZT) detectors, which have recently demonstrated high-resolution capabilities for hard X-ray and gamma-ray detection. A demonstrator prototype based on a new cadmium-zinc-telluride quasi-hemispherical detector and custom digital pulse processing electronics was developed. The detector covered a detection area of 1 cm2 with a single readout channel and interesting room-temperature performance with energy resolution of 4.4% (2.6 keV), 3% (3.7 keV), and 1.4% (9.3 keV) FWHM at 59.5, 122.1, and 662 keV, respectively. The results from X-ray measurements at the DAΦNE collider at the INFN National Laboratories of Frascati (Italy) are also presented with particular attention to the effects and rejection of electromagnetic and hadronic background

CdZnTe detectors tested at the DA{\Phi}NE collider for future kaonic atoms measurements

The SIDDHARTA-2 collaboration at the INFN Laboratories of Frascati (LNF) aims to perform groundbreaking measurements on kaonic atoms. In parallel and beyond the ongoing kaonic deuterium, presently running on the DA$\Phi$NE collider at LNF, we plan to install additional detectors to perform further kaonic atoms' studies, taking advantage of the unique low energy and low momentum spread $K^-$ beam delivered by the at-rest decay of the $\phi$ meson. CdZnTe devices are ideal for detecting transitions toward both the upper and lower levels of intermediate-mass kaonic atoms, like kaonic carbon and aluminium, which have an important impact on the strangeness sector of nuclear physics. We present the results obtained in a set of preliminary tests conducted on DA$\Phi$NE, in view of measurements foreseen in 2024, with the twofold aim to tune the timing window required to reject the extremely high electromagnetic background, and to quantify the readout electronics saturation effect due to the high rate, when placed close to the Interaction Region (IR). In the first test we used commercial devices and electronics, while for the second one both were customized at the IMEM-CNR of Parma and the University of Palermo. The results confirmed the possibility of finding and matching a proper timing window where to identify the signal events and proved better performances, in terms of energy resolution, of the custom system. In both cases, strong saturation effects were confirmed, accounting for a loss of almost 90\% of the events, which will be overcome by a dedicated shielding structure foreseen for the final experimental setup

New opportunities for kaonic atoms measurements from CdZnTe detectors

We present the tests performed by the SIDDHARTA-2 collaboration at the DA Φ NE collider with a quasi-hemispherical CdZnTe detector. The very good room-temperature energy resolution and efficiency in a wide energy range show that this detector technology is ideal for studying radiative transitions in intermediate and heavy mass kaonic atoms. The CdZnTe detector was installed for the first time in an accelerator environment to perform tests on the background rejection capabilities, which were achieved by exploiting the SIDDHARTA-2 Luminosity Monitor. A spectrum with an 241Am source has been acquired, with beams circulating in the main rings, and peak resolutions of 6% at 60 keV and of 2.2% at 511 keV have been achieved. The background suppression factor, which turned out to be of the order of ≃ 10 5 - 6 , opens the possibility to plan for future kaonic atom measurements with CdZnTe detectors