Expression of mucoid induction factor MucE is dependent upon the alternate sigma factor AlgU in Pseudomonas aeruginosa

Background Alginate overproduction in P. aeruginosa, also referred to as mucoidy, is a poor prognostic marker for patients with cystic fibrosis (CF). We previously reported the construction of a unique mucoid strain which overexpresses a small envelope protein MucE leading to activation of the protease AlgW. AlgW then degrades the anti-sigma factor MucA thus releasing the alternative sigma factor AlgU/T (σ22) to initiate transcription of the alginate biosynthetic operon. Results In the current study, we mapped the mucE transcriptional start site, and determined that PmucE activity was dependent on AlgU. Additionally, the presence of triclosan and sodium dodecyl sulfate was shown to cause an increase in PmucE activity. It was observed that mucE-mediated mucoidy in CF isolates was dependent on both the size of MucA and the genotype of algU. We also performed shotgun proteomic analysis with cell lysates from the strains PAO1, VE2 (PAO1 with constitutive expression of mucE) and VE2ΔalgU (VE2 with in-frame deletion of algU). As a result, we identified nine algU-dependent and two algU-independent proteins that were affected by overexpression of MucE. Conclusions Our data indicates there is a positive feedback regulation between MucE and AlgU. Furthermore, it seems likely that MucE may be part of the signal transduction system that senses certain types of cell wall stress to P. aeruginosa

Invariant Two-Component Structure of the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS)

The Repeatable Battery for the Assessment of Neuropsychological Status is a brief neurocognitive instrument used to evaluate cognitive functioning in clinical settings. Prior investigations of the factor structure have revealed subtle differences across samples. It was hypothesized that these differences are primarily the result of methodological decisions made by researchers. The present study utilized empirically supported extraction criteria (parallel analysis; minimum average partial procedure) and uniformly investigated 5 samples. RBANS data from 4 previously published studies (Carlozzi, Horner, Yang, & Tilley, 2008; Duff, Hobson, Beglinger, & O\u27Bryant, 2010; Duff et al., 2006; Wilde, 2006) were reanalyzed, and a new clinical sample was obtained from the Gundersen Health System Memory Center. The congruence of factor structures was investigated by conducting orthogonal vector matrix comparisons (Barrett, 2005), and a robust 2-factor structure reliably emerged across samples. The invariant RBANS 2-factor structure primarily emphasized memory and visuospatial functioning. This finding offered further support for a 2-factor RBANS structure identified in previous studies and additionally provided empirical documentation of replication across diverse samples. Due to the expansive use of the RBANS, this psychometric knowledge has significant clinical implications. It should facilitate accurate interpretation of test data and allow clinicians to make more informed decisions regarding whether the instrument is appropriate to use in various clinical settings

The Grizzly, March 2, 1998

Ursinus Offers Free Practice GRE Exam to Students • Eating Disorder Screening Offered at Wellness • A Meeting Educates UC • Texas Winners Tell All • Letters to the Editor: Greeks Respond to Rudolfs Article; Response to New Bookstore • A Tribe Called Graffiti • Safety Issues Crossing Main Street • Gross Honored in Who\u27s Who • Sexual Assault Awareness Week • Outdoor Adventure Club Comes to Ursinus • Bears Run Into a Wall at Hopkins • Solid Performance at Championships for UC Swimming • Indoor Track Championships • Tribute to the Unsung Hero • USA D-III Mardi Gras All-Star Classic • UC Gymnasts Headed All the Way to the Tophttps://digitalcommons.ursinus.edu/grizzlynews/1416/thumbnail.jp

The Grizzly, April 28, 1998

Cornel West to Speak at Commencement • Ursinus Class of \u2748 to Meet Class of \u2798 • Ursinus Security Officer Survives Motorcycle Accident • Editors Say Farewell • Politics Department Bids Farewell • Reflections From the Class of 1998 • Opinion: On Behalf of International Students • Recognition of the Arts at UC • A Minister\u27s Homecoming • The Great Pennsylvania Workout Day Comes to Ursinus • New Honor For Ursinus • Plans for New Fieldhouse Underway • Spring Fling • Ursinus Students Escape Velocity • Newt Recognizes Students • 1998 Graduation Activities • Ursinus Lacrosse Defeats Haverford College • Women\u27s Tennis: One Last Try • Softball Looking Strong in the Home Stretch • Baseball Closes Out Centennial Conference Play • UC Has Field Day at Millersville • Ursinus\u27 Tribute to Our Senior Spring Athleteshttps://digitalcommons.ursinus.edu/grizzlynews/1421/thumbnail.jp

Correction and lengthening for deformities of the forearm in multiple cartilaginous exostoses

金沢大学医学部附属病院整形外科Background. Multiple cartilaginous exostoses cause various deformities of the epiphysis. In exostoses of the ulna, the ulna is shortened and the radius acquires varus deformity, which may lead to dislocation of the radial head. In this study, we present the results of exostoses resection, with correction and lengthening with external fixators for functional and cosmetic improvement, and prevention of radial head dislocation. Methods. We retrospectively reviewed seven forearms of seven patients who had deformities of the forearm associated with multiple cartilaginous exostoses. One patient had dislocation of the radial head. Operative technique was excision of osteochondromas from the distal ulna, correction of the radius, and ulnar lengthening with external fixation up to 5 mm plus variance. We evaluated radiographs and the range of pronation and supination. Furthermore, we conducted a follow-up of ulnar length after the operation. Results. Dislocation of the radial head of one patient was naturally reduced without any operative intervention. At the most recent follow-up, six of the seven patients showed full improvement in pronation-supination. Ulnar shortening recurred with skeletal growth of four skeletally immature patients; however, it did not recur in one skeletally mature patient. Overlength of 5 mm was negated by the recurrence of ulnar shortening about 1.5 years after the operation. Conclusions. We treated seven forearms of seven patients by excision of osteochondromas, correction of radii, and gradual lengthening of ulnas with external fixators. The results of the procedure were satisfactory, especially for function of the elbow and wrist. However, we must consider the possible recurrence of ulnar shortening within about 1.5 years during skeletal growth periods in immature patients. © 2006 The Japanese Orthopaedic Association

Using surveillance data to determine treatment rates and outcomes for patients with chronic hepatitis C virus infection

The aim of this work was to develop and validate an algorithm to monitor rates of, and response to, treatment of patients infected with hepatitis C virus (HCV) across England using routine laboratory HCV RNA testing data. HCV testing activity between January 2002 and December 2011 was extracted from the local laboratory information systems of a sentinel network of 23 laboratories across England. An algorithm based on frequency of HCV RNA testing within a defined time period was designed to identify treated patients. Validation of the algorithm was undertaken for one center by comparison with treatment data recorded in a clinical database managed by the Trent HCV Study Group. In total, 267,887 HCV RNA test results from 100,640 individuals were extracted. Of these, 78.9% (79,360) tested positive for viral RNA, indicating an active infection, 20.8% (16,538) of whom had a repeat pattern of HCV RNA testing suggestive of treatment monitoring. Annual numbers of individuals treated increased rapidly from 468 in 2002 to 3,295 in 2009, but decreased to 3,110 in 2010. Approximately two thirds (63.3%; 10,468) of those treated had results consistent with a sustained virological response, including 55.3% and 67.1% of those with a genotype 1 and non-1 virus, respectively. Validation against the Trent clinical database demonstrated that the algorithm was 95% sensitive and 93% specific in detecting treatment and 100% sensitive and 93% specific for detecting treatment outcome. Conclusions: Laboratory testing activity, collected through a sentinel surveillance program, has enabled the first country-wide analysis of treatment and response among HCV-infected individuals. Our approach provides a sensitive, robust, and sustainable method for monitoring service provision across Englan

Real and Monetary Policy Convergence: EMU Crisis to the CFA Zone

A major lesson of the EMU crisis is that serious disequilibria result from regional monetary arrangements not designed to be robust to a variety of shocks. The purpose of this paper is to assess these disequilibria within the CEMAC, UEMOA and CFA zones. In the assessments, monetary policy targets inflation and financial dynamics of depth, efficiency, activity and size while real sector policy targets economic performance in terms of GDP growth. We also provide the speed of convergence and time required to achieve a 100% convergence. But for financial intermediary size within the CFA zone, findings for the most part support only unconditional convergence. There is no form of convergence within the CEMAC zone. The broad insignificance of conditional convergence results have substantial policy implications. Monetary and real policies which are often homogenous for member states are thwarted by heterogeneous structural and institutional characteristics which give rise to different levels and patterns of financial intermediary development. Therefore member states should work towards harmonizing cross-country differences in structural and institutional characteristics that hamper the effectiveness of monetary policies

Are Proposed African Monetary Unions Optimal Currency Areas? Real, Monetary and Fiscal Policy Convergence Analysis

Purpose – A spectre is hunting embryonic African monetary zones: the EMU crisis. This paper assesses real, monetary and fiscal policy convergence within the proposed WAM and EAM zones. The introduction of common currencies in West and East Africa is facing stiff challenges in the timing of monetary convergence, the imperative of central bankers to apply common modeling and forecasting methods of monetary policy transmission, as well as the requirements of common structural and institutional characteristics among candidate states. Design/methodology/approach – In the analysis: monetary policy targets inflation and financial dynamics of depth, efficiency, activity and size; real sector policy targets economic performance in terms of GDP growth at macro and micro levels; while, fiscal policy targets debt-to-GDP and deficit-to-GDP ratios. A dynamic panel GMM estimation with data from different non-overlapping intervals is employed. The implied rate of convergence and the time required to achieve full (100%) convergence are then computed from the estimations. Findings – Findings suggest overwhelming lack of convergence: (1) initial conditions for financial development are different across countries; (2) fundamental characteristics as common monetary policy initiatives and IMF backed financial reform programs are implemented differently across countries; (3) there is remarkable evidence of cross-country variations in structural characteristics of macroeconomic performance; (4) institutional cross-country differences could also be responsible for the deficiency in convergence within the potential monetary zones; (5) absence of fiscal policy convergence and no potential for eliminating idiosyncratic fiscal shocks due to business cycle incoherence. Practical implications – As a policy implication, heterogeneous structural and institutional characteristics across countries are giving rise to different levels and patterns of financial intermediary development. Thus, member states should work towards harmonizing cross-country differences in structural and institutional characteristics that hamper the effectiveness of convergence in monetary, real and fiscal policies. This could be done by stringently monitoring the implementation of existing common initiatives and/or the adoption of new reforms programs. Originality/value – It is one of the few attempts to investigate the issue of convergence within the proposed WAM and EAM unions