Antimicrobial Finish of Polyethersulfone Membranes: Sticking Photosensitizers‐Like Marine Mussels Would Do

Biofouling is one of the most serious problems in membrane filtration systems for water treatment. An easily applicable antimicrobial finish of polyethersulfone (PES) filter membranes is shown by derivatizing an approved chlorin photosensitizer drug with adhesive ortho-catechol functionalities as known from l-3,4-dihydroxyphenylalanin (Dopa) residues of marine mussel glues. The chemical structure of the second-generation photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)chlorin (m-THPC) is modified by 2-iodoxybenzoic acid (IBX) oxidation, transforming the peripheric meta-phenols of m-THPC into ortho-catechols, and thus improving the adsorption properties of m-THPC derivatives onto PES material. Stable coatings are formed that preserve chlorin's capability to generate singlet oxygen under visible light illumination. The modification of one or two phenol groups out of the four m-THPC substituents leads to an optimum in the generation of active singlet oxygen, and thus the finish significantly reduces the bacterial growth of gram-negative Escherichia coli and gram-positive Micrococcus luteus on the PES membranes.Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659Peer Reviewe

Flow cytometry-based FRET identifies binding intensities in PPAR[gamma]1 protein-protein interactions in living cells

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