Rapid isolation and purification of mitochondria for transplantation using tissue dissociation and differential filtration

Researchers have identified several methods for treating acute myocardial infarction (AMI) patients affected by ischemia and reperfusion injury. Some of these therapies include thrombolysis, balloon angioplasty, and coronary arterial bypass graft (CAGB). This lab has previously demonstrated that transplantation of mitochondria into the ischemic zone of a rabbit heart during reperfusion significantly improved recovery as compared to current techniques. In order for this therapy to be translated into the clinic a rapid isolation method for producing highly pure and functional mitochondria will be required. Previously described mitochondrial isolation methods using differential centrifugation and/or Ficoll gradient centrifugation require 60 to 100 minutes to complete. Herein, a method for rapid isolation of mitochondria from mammalian tissue biopsies is described. In this protocol, manual homogenization is replaced with the tissue dissociator's standardized homogenization cycle. This allows for uniform and consistent homogenization of tissue that is not easily achieved with manual homogenization. Following tissue dissociation, the homogenate is filtered through nylon mesh filters which eliminates repetitive centrifugation steps. Mitochondrial isolation time is less than 30 minutes compared to 60-100 minutes using alternative methods. This isolation protocol yields approximately 2 x 10^10 viable and respiration competent mitochondria from 0.18 ± 0.04 g (wet weight) tissue sample

Learning the Right Lessons from Iraq

Foreign policy experts and policy analysts are misreading the lessons of Iraq. The emerging conventional wisdom holds that success could have been achieved in Iraq with more troops, more cooperation among U.S. government agencies, and better counterinsurgency doctrine. To analysts who share these views, Iraq is not an example of what not to do but of how not to do it. Their policy proposals aim to reform the national security bureaucracy so that we will get it right the next time. The near-consensus view is wrong and dangerous. What Iraq demonstrates is a need for a new national security strategy, not better tactics and tools to serve the current one. By insisting that Iraq was ours to remake were it not for the Bush administration's mismanagement, we ignore the limits on our power that the war exposes and in the process risk repeating our mistake. The popular contention that the Bush administration's failures and errors in judgment can be attributed to poor planning is also false. There was ample planning for the war, but it conflicted with the Bush administration's expectations. To the extent that planning failed, therefore, the lesson to draw is not that the United States national security establishment needs better planning, but that it needs better leaders. That problem is solved by elections, not bureaucratic tinkering. The military gives us the power to conquer foreign countries, but not the power to run them. Because there are few good reasons to take on missions meant to resuscitate failed governments, terrorism notwithstanding, the most important lesson from the war in Iraq should be a newfound appreciation for the limits of our power

Preble Stolz Letter

Professor Stolz is interested in an association of law teachers but also addresses concerns

Language as Aperture

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The Sunsat Act - Transforming our Energy, Economy and Environment

Our global economy depends on low cost energy. In reaction to peaking oil prices, our economy is in a shambles. We must rebuild our energy supply. Many energy alternatives have been explored and subsidized since the Arab Oil embargo shock of 1973, yet our oil, gas and energy dependency has grown. Our energy security is declining. Rebuilding our primary energy supply is hard. Fortunately, technology has opened the door to a clean new baseload energy player, Space Solar Power (SSP). The difference between communication satellites (comsats) now in use and the power satellites (sunsats) we need, is that sunsats would optimize for efficient power transfer, while comsats have optimized their signal to noise ratio. Just as the Comsat Act of 1962 created our robust commercial satellite communications industry, the key legislation that would enable SSP to become a major energy source is entitled the Sunsat Act. The Sunsat Act would create a commercial power satellite industry

The Shame Game: Montana\u27s Right To Privacy For Level 1 Sex Offenders

The Shame Game: Montana\u27s Right To Privacy For Level 1 Sex Offender

Obesity and Malnutrition

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