Rapid isolation and purification of mitochondria for transplantation using tissue dissociation and differential filtration

Researchers have identified several methods for treating acute myocardial infarction (AMI) patients affected by ischemia and reperfusion injury. Some of these therapies include thrombolysis, balloon angioplasty, and coronary arterial bypass graft (CAGB). This lab has previously demonstrated that transplantation of mitochondria into the ischemic zone of a rabbit heart during reperfusion significantly improved recovery as compared to current techniques. In order for this therapy to be translated into the clinic a rapid isolation method for producing highly pure and functional mitochondria will be required. Previously described mitochondrial isolation methods using differential centrifugation and/or Ficoll gradient centrifugation require 60 to 100 minutes to complete. Herein, a method for rapid isolation of mitochondria from mammalian tissue biopsies is described. In this protocol, manual homogenization is replaced with the tissue dissociator's standardized homogenization cycle. This allows for uniform and consistent homogenization of tissue that is not easily achieved with manual homogenization. Following tissue dissociation, the homogenate is filtered through nylon mesh filters which eliminates repetitive centrifugation steps. Mitochondrial isolation time is less than 30 minutes compared to 60-100 minutes using alternative methods. This isolation protocol yields approximately 2 x 10^10 viable and respiration competent mitochondria from 0.18 ± 0.04 g (wet weight) tissue sample

Learning the Right Lessons from Iraq

Foreign policy experts and policy analysts are misreading the lessons of Iraq. The emerging conventional wisdom holds that success could have been achieved in Iraq with more troops, more cooperation among U.S. government agencies, and better counterinsurgency doctrine. To analysts who share these views, Iraq is not an example of what not to do but of how not to do it. Their policy proposals aim to reform the national security bureaucracy so that we will get it right the next time. The near-consensus view is wrong and dangerous. What Iraq demonstrates is a need for a new national security strategy, not better tactics and tools to serve the current one. By insisting that Iraq was ours to remake were it not for the Bush administration's mismanagement, we ignore the limits on our power that the war exposes and in the process risk repeating our mistake. The popular contention that the Bush administration's failures and errors in judgment can be attributed to poor planning is also false. There was ample planning for the war, but it conflicted with the Bush administration's expectations. To the extent that planning failed, therefore, the lesson to draw is not that the United States national security establishment needs better planning, but that it needs better leaders. That problem is solved by elections, not bureaucratic tinkering. The military gives us the power to conquer foreign countries, but not the power to run them. Because there are few good reasons to take on missions meant to resuscitate failed governments, terrorism notwithstanding, the most important lesson from the war in Iraq should be a newfound appreciation for the limits of our power

Islamic Extremism in Northern Africa, Case Study: Nigeria and Boko Haram

This event will feature three panelists: Christopher Preble of the CATO Institute, and Kelechi Kalu and Ousman M. Kobo of The Ohio State University, who will discuss the rise of Islamic extremism in Northern Africa, with an emphasis on Nigeria and Boko Haram.Ohio State UniversityMershon Center for International Security StudiesAlexander Hamilton SocietyEvent Web page, event photo

On-Chip Quantum Interference from a Single Silicon Ring Resonator Source

Here we demonstrate quantum interference of photons on a Silicon chip produced from a single ring resonator photon source. The source is seamlessly integrated with a Mach-Zehnder interferometer, which path entangles degenerate bi-photons produced via spontaneous four wave mixing in the Silicon ring resonator. The resulting bi-photon N00N state is controlled by varying the relative phase of the integrated Mach-Zehnder interferometer, resulting in high two-photon interference visibilities of V~96%. Furthermore, we show that the interference can be produced using pump wavelengths tuned to all of the ring resonances accessible with our tunable lasers (C+L band). This work is a key demonstration towards the simplified integration of multiple photon sources and quantum circuits together on a monolithic chip, in turn, enabling quantum information chips with much greater complexity and functionality

Thermal surveillance of Cascade Range volcanoes using ERTS-1 multispectral scanner, aircraft imaging systems, and ground-based data communication platforms

A combination of infrared images depicting areas of thermal emission and ground calibration points have proved to be particularly useful in plotting time-dependent changes in surface temperatures and radiance and in delimiting areas of predominantly convective heat flow to the earth's surface in the Cascade Range and on Surtsey Volcano, Iceland. In an integrated experiment group using ERTS-1 multispectral scanner (MSS) and aircraft infrared imaging systems in conjunction with multiple thermistor arrays, volcano surface temperatures are relayed daily to Washington via data communication platform (DCP) transmitters and ERTS-1. ERTS-1 MSS imagery has revealed curvilinear structures at Lassen, the full extent of which have not been previously mapped. Interestingly, the major surface thermal manifestations at Lassen are aligned along these structures, particularly in the Warner Valley

Fully quantum mechanical dynamic analysis of single-photon transport in a single-mode waveguide coupled to a traveling-wave resonator

We analyze the dynamics of single photon transport in a single-mode waveguide coupled to a micro-optical resonator using a fully quantum mechanical model. We examine the propagation of a single-photon Gaussian packet through the system under various coupling conditions. We review the theory of single photon transport phenomena as applied to the system and we develop a discussion on the numerical technique we used to solve for dynamical behavior of the quantized field. To demonstrate our method and to establish robust single photon results, we study the process of adiabatically lowering or raising the energy of a single photon trapped in an optical resonator under active tuning of the resonator. We show that our fully quantum mechanical approach reproduces the semi-classical result in the appropriate limit and that the adiabatic invariant has the same form in each case. Finally, we explore the trapping of a single photon in a system of dynamically tuned, coupled optical cavities.Comment: 24 pages, 10 figure