Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues

<p>Abstract</p> <p>Background</p> <p>Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification of proteins resembling the tumor biology can improve the diagnosis, prediction, treatment selection, and targeting of therapy. Since the beginning of the post-genomic era, the focus of molecular biology gradually moved from genomes to proteins and proteomes and to their functionality. Proteomics can potentially capture dynamic changes in protein expression integrating both genetic and epigenetic influences.</p> <p>Methods</p> <p>We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis. Seven patients developed distant metastases within three-year follow-up. These samples were included into a metastase-positive group, the others formed a metastase-negative group. Two-dimensional electrophoretical (2-DE) gels in pH range 4–7 were prepared. Spot densities in 2-DE protein maps were subjected to statistical analyses (R/maanova package) and data-mining analysis (GUHA). For identification of proteins in selected spots, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.</p> <p>Results</p> <p>Three protein spots were significantly altered between the metastatic and non-metastatic groups. The correlations were proven at the 0.05 significance level. Nucleophosmin was increased in the group with metastases. The levels of 2,3-trans-enoyl-CoA isomerase and glutathione peroxidase 1 were decreased.</p> <p>Conclusion</p> <p>We have performed an extensive proteomic study of mammary epithelial cells from breast cancer patients. We have found differentially expressed proteins between the samples from metastase-positive and metastase-negative patient groups.</p

Acoustic study of fish and invertebrate behavior in a tropical reservoir

The fish and invertebrate behavior of the Ubol Ratana Reservoir, Thailand, were monitored using up- and downlooking split beam sonar located at a fixed location. In the same area and period, ichthyoplankton nets and multimesh gillnets were used. The bulk of targets, recorded by acoustics and direct capture, consisted both of fish 3–4 cm long and insect larvae 0.2–1 cm long. Diurnal patterns of behavior were very distinct: during the daytime, invertebrates were hidden in the bottom and most fish stayed in compact shoals. Time course of acoustic fish biomass and abundance was very variable due to shoaling. Only the largest fish were recorded as solitary targets. At night, the whole acoustic range was filled with targets and the time course of fish biomass (5–15 kg ha–1) and abundance (20–45 thousand individuals ha–1) were more constant. The biomass increased mostly at surface layers. Fish appeared in the evening in the water column 1 h earlier and stayed there in the morning 1 h longer than invertebrates. Dawn and dusk are good periods for studying fish before invertebrates outnumber them. Apart from fish, according to the target strength, swimming speed and depth distribution, at least four groups of water invertebrates were distinguished acoustically, some with extremely fast vertical movement (7–9 cm s–1 vertical speed). Comparison of up- and downlooking observations gave comparable results in midwater layer outside the near-field of the transducer. The uplooking approach can be more suitable for night records; downlooking for the day [KEYWORDS: Acoustics; Echospecies; Target strength; Acoustic tracking and identification; Behavioral pattern]

Tracking aquatic animals to understand a world increasingly shaped by a changing climate and extreme weather events

Despite great promise for understanding the impacts and extent of climate change on aquatic animals, their species, and ecological communities, it is surprising that tracking tools, like biotelemetry and biologging devices, have not been extensively used to understand climate change or develop and evaluate potential interventions that may forestall or mitigate its effects. In this review, we provide an overview of methodologies and study designs that leverage available tracking tools to investigate aspects of climate change on aquatic ecosystems. Key interventions to protect aquatic life from the impacts of climate change, including habitat restoration, protected areas, conservation translocations, mitigations against interactive effects of climate change, and simulation of future scenarios can all be greatly facilitated by using electronic tagging and tracking. We anticipate that adapting study designs (e.g. use of replicated ponds, randomized control trials, physiologging) to effectively use tracking will greatly enhance our understanding of climate change and its impacts on aquatic ecosystems, hopefully also facilitating research into effective solutions and interventions against the most extreme and acute impacts