A NEW RECORD OF THE RHEOPHYTIC FERN Osmunda angustifolia (OSMUNDACEAE) FROM SUMATRA INCLUDING A NEW CYTOLOGICAL RECORD

Osmunda angustifolia Ching ex Ching et Wang (Osmundaceae) was, formerly, known to be distributed in China (Guandong), Hainan, Hongkong, and Taiwan. Now, O. angustifolia is reported as a newly recorded species in Sumatra. A complete morphological description with photographs is presented. It is defined as a rare species in Indonesia. A cytological record is also reported for the first time for the Sumatran species. The somatic chromosome number of root tip cells of O. angustifolia is 2n = 44 (diploid)

AKLIMATISASI DINI MASSA PROTALUS TUMBUHAN PAKU BAHAN OBAT (Cibotium barometz (L.) J. Sm.) HASIL KULTUR SPORA SECARA IN VITRO

The golden chicken fern, Cibotium barometz (L.) J. Sm (Cibotiaceae), is an important export commodity used in both traditional and modern medicines. The population of C. barometz in some countries has declined rapidly due to over-exploitation. Therefore, this species has been included in Appendix II of CITES (Convention on International Trade in Endangered Species) since 1976. The Center for Plant Conservation – Bogor Botanic Gardens-LIPI, is striving to conserve this species ex situ, and has started propagating it through in vitro spore culture. However, C. barometz sporophyte formation from in vitro cultured spores takes a long time. Thus, we sought to acclimatize prothalli masses (gametophytes) that had not yet developed sporophytes, in an attempt to accelerate sporophyte formation. Acclimatization experiments were carried out in two stages. The first experiment used prothalli masses aged 8 months after sowing, and tested 14 kinds of acclimatization media in a transparant plastic box. The second experiment tested prothalli masses aged 15 months with the four best media selected from the first experiment, namely: (1) minced roots of the tree fern Cyathea contaminans (APC); (2) APC: charcolaled rice husk (ASP) in a 1: 1 ratio; (3) APC: cocopeat (CP) in a 1:1 ratio, and (4) APC: ASP:CP (1:1:1), using a plastic box with a translucent plastic lid. Results of the second experiment showed that the best medium for acclimatization is a mixed media composed of minced roots of the tree fern Cyathea contaminans, charcoaled rice husk, and cocopeat (1:1:1). Up to 80% of the prothalli masses grew on the three ingredient mixed media, resulting in the formation of as many as 574 sporophytes

ANATOMI PARADERMAL DAUN ENAM JENIS TUMBUHAN PAKU MARGA Pteris

Pteris is a cosmopolitant fern genus inhabiting many kinds of habitats, mostly in forests. The genus grows as terrestrial or lithophyte. The high species diversity of Pteris was resulted from hybridization, apogamy or polyploidy, therefore complex species with continuous morphological characteristics often occur. This study was carried out to determine the role of paradermal anatomical characteristics as supporting data for species delimitation of Pteris. The study was done by observing anatomical characteristics of Pteris, namely stomatal size, epidermal shape and size, amplitude and wave length of sinousity, stomatal index and density, and the distribution of stomatal type. Six species of Pteris were observed, namely P. ensiformis Burm.f., P. fauriei Hieron., P. heteromorpha Fée, P. longipinnnula Wall. ex J. Agardh, P. tripartita Sw. and P. vittata L. The average of stomatal and epidermal size, as well as amplitude and wave length of anticlinal wall of the epidermal cells were relatively different for each species. The average of stomatal index and density were also different among species. Eight stomatal types were found to be distributed among the abaxial epidermis cells of Pteris. Six stomatal types were newly recorded for Pteris, namely anomocytic, pseudocopolocytic, hemiparacytic, cohemiparacytic, diacytic and brachyparacytic. The eight stomatal types were distributed among species with different combination and percentage of occurence. The quantitative characteristics of paradermal anatomy was not strong enough to support species delimitation in Pteris. However, the qualitative characteristics of paradermal anatomy seemed to be useful to support species delimitation of Pteris

Inventorying of the tree fern Genus Cibotium of Sumatra: Ecology, population size and distribution in North Sumatra

Praptosuwiryo TNg, Pribadi DO, Puspitaningtyas DM, Hartini S (2011) Inventorying the tree fern Genus Cibotium of Sumatra: Ecology, population size and distribution in North Sumatra. Biodiversitas 12: 204-211. Cibotium is one tree fern belongs to the family Cibotiaceae which is easily differentiated from the other genus by the long slender golden yellowish-brown smooth hairs covered its rhizome and basal stipe with marginal sori at the ends of veins protected by two indusia forming a small cup round the receptacle of the sorus. It has been recognized as material for both traditional and modern medicines in China, Europe, Japan and Southeast Asia. Population of Cibotium species in several countries have decreased rapidly because of over exploitation and there is no artificial cultivation until now. The aims of this study were: (i) To re-inventory the species of Cibotiun in North Sumatra, (ii) to record the ecology and distribution of each species, and (iii) to assess the population size of each species. Field study was carried out by using random search with belt transect. Two species were recorded, namely C. arachnoideum dan C. barometz. The geographical distribution of the two species in North Sumatra are presented. Cibotium is commonly growing terrestrially on opened or rather opened areas in secondary forets and primary forest at hills or lower mountains with a relatively high humidity at 30-90º slopes. C. arachnoideum has a strict distribution and only found at 1740-1770 m a.s.l. in primary forest, whereas C. barometz has a broad distribution in secondary forest at elevation range from 650-1200 m

Spore germination and early gametophyte development of Platycerium wandae (Polypodiaceae) from Papua, Indonesia

Abstract. Praptosuwiryo TNg. 2017. Spore germination and early gametophyte development of Platycerium wandae (Polypodiaceae) from Papua, Indonesia. Biodiversitas 18: 175-182. Studies on gametophyte development in ferns are very important, as the data generated is often useful for supporting taxa delimitation in ferns and fern-allies. This data is also very important for understanding the ecology, reproductive biology, evolution and distribution of ferns. The study reported here aimed to develop a deeper understand of morphogenesis in the fern Platycerium wandae Racib. by investigating the process of spore germination and early gametophyte development of the species on natural media. Fresh spores of P. wandae were germinated in a mixed media consisting of minced roots of Cyathea contaminans and charcoaled rice hulls (1: 1) under green house condition. Spores of P. wandae are monolete, ellipsoid, non-chlorophyllous, dark brown, and lacking perine. Spores germinated between 7 to 14 days after sowing (DAS). Spore germination of P. wandae is of the Vittaria-type and the prothallial development is of the Aspidium-type, characterized by early development of unicelullar trichomes (30-40 DAS). Only unisexual gametophytes were observed at 60-80 DAS. New data is provided concerning the morphogenesis of P. wandae from Papua, Indonesia, on natural media. The findings are relevant to reproductive biology, would contribute to establish an efficient ex situ propagation strategies for the conservation of epiphyitic ferns and to facilitate further in situ studies of gametophyte ecology. Keywords: Gametophyte development, Platycerium wandae, spore germination, staghorn fer

A New Record Of The Rheophytic Fern Osmunda Angustifolia (Osmundaceae) From Sumatra Including A New Cytological Record

Osmunda angustifolia Ching ex Ching et Wang (Osmundaceae) was, formerly, known to be distributed in China (Guandong), Hainan, Hongkong, and Taiwan. Now, O. angustifolia is reported as a newly recorded species in Sumatra. A complete morphological description with photographs is presented. It is defined as a rare species in Indonesia. A cytological record is also reported for the first time for the Sumatran species. The somatic chromosome number of root tip cells of O. angustifolia is 2n = 44 (diploid)

Mengenal Microsorum scolopendria (Burm. F.) Copel (Polypodiaceae) Paku Liar di Kebun Raya Bogor

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