Relaparotomy after caesarean section: a retrospective observational study in a tertiary medical college

Background: Relaparotomy is the term which defines operations performed within 60days after the initial surgery. Ralaparotomy following caesarean section may happen and that’s why a study was done to evaluate the risk factors, indications, procedures done during relaparotomy after caesarean section.Methods: A retrospective observational study was conducted in the department of obstetrics and gynaecology in burdwan medical college and hospital for a period of 3years from 1June 2019 to 31 May 2022. Total 32 cases required relaparotomy following caesarean section.Results: In this study, there were 25,527 caesarean deliveries out of 56,145 total deliveries over 3years time period. Among caesarean deliveries 32 cases underwent relaparotomy (0.12%). In most of the cases relaparotomies were due to intraperitoneal haemorrhage, rectus sheath hematoma, postpartum haemorrhage. Indications of cesarean section were severe preeclampsia, non-progress of labour, abruption, post cesarean section with scar tenderness, meconium-stained liquor with fetal distress. Most of the women were in the age group of 20-30 years. Resuturing of the bleeding points were done in most of the cases.Conclusions: As a lifesaving procedure, decision of relaparotomy should be taken as soon as possible. Proper hemostasis need to be ensured before closure of abdomen, as intraperitoneal haemorrhage is the most common indications for relaparotomy

Maternal mortality-a three-year retrospective study in a rural medical college of India

Background: Maternal mortality is a tragic event as family revolves around a mother. The deadly obstetrical triad of hemorrhage, preeclampsia and infection has accounted for a third of all deaths. This study was conducted to assess maternal mortality ratio, demographic profile and causes of maternal death.Methods: This retrospective longitudinal study was conducted in the department of obstetrics and gynecology for a period of three years from 1st January 2018-31st December, 2020. Total no of death during this period was 134.Records of deaths and demographic profiles were retrieved from the medical record library of aforesaid hospital.Results: There were 134 maternal deaths amongst 56815 live births with MMR 235.85. The majority of deaths were in 20-29 year of age group and most of the deaths seen in multigravida. The 91.79% death was observed within the 24 hours and after 72 hours. Eclampsia, preeclampsia and hemorrhage were leading cause of maternal death seen in the study.Conclusions: Maternal mortality is higher than national MMR. Majority of maternal death were preventable by proper antenatal care, early detection of high-risk pregnancies and their timely referral to tertiary care centre

Assessment of genetic diversity using morphological and molecular markers in traditional cultivars of Mango (Mangifera indica L.)

404-413Mango (Mangifera indica L.) is an economically important tropical and subtropical fruit crop consumed worldwide. Alloploidy, nature of cross-pollination and a wide range of predominant agro-ecologies of the country have contributed large genetic diversity of mango in India. The present study assessed 16 traditional mango cultivars to get a unique insight on cultivars' diversity through deploying integration of both morphological and molecular markers. The cultivars were appraised for consecutive two years under the aegis of All India Coordinated Research Project on Fruits, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Gayeshpur, West Bengal regarding observation on 26 morphological and fruit quality parameters followed by assessing diversity at molecular level through deploying 20 SSR makers. Presence of adequate genetic variability was reflected for all the tested traits. Principal Component Analysis (PCA) ascertained seven PCs towards contribution of more than 84.25% genetic diversity harbored by the tested cultivars. Out of 20 SSRs, 8 microsatellites were amplified and produced 27 putative alleles in 16 cultivars. Genetic divergence through multivariate analysis, as well as through UPGMA dendrograms, classified 16 mango cultivars into five major clusters, though, the cluster composition was different. The dendrogram affirmed that the highest similarity (88%) was observed in between Ranipasand and Gulab Khas. Sharing of common gene pool coupled with exertion of similar selection pressure during domestication as well as selection of cultivars in this region exhibited similar tradition

Electron acoustic shock waves in nonisothermal dissipative plasmas

The propagation characteristics of weakly nonlinear electron acoustic waves in the presence of nonisothermal (trapped) hot electrons are investigated in collisional plasmas. The dynamics of the nonlinear waves are found to be governed by Schamel–Burgers and Schamel–Korteweg–de Vries–Burgers-type equations depending on the strength of the nonisothermal parameter. Burgers’ terms appear due to the anomalous dissipation introduced by the collisions between cold electrons and immobile ions in the presence of collective phenomena (plasma current). The derived nonlinear equations are solved analytically with the help of the Tanh method. The time-dependent computational results well agree with the analytical results and predict the possibility of the oscillatory and monotonic shock-like structures depending on the strength of the collisional drag and nonisothermality of hot electrons. The trapped electrons significantly modify the amplitude and width of the nonlinear pulse. The results may explain the shock formation and the particle acceleration mechanism in auroral plasma region

Assessment of genetic diversity using morphological and molecular markers in traditional cultivars of Mango (Mangifera indica L.)

Mango (Mangifera indica L.) is an economically important tropical and subtropical fruit crop consumed worldwide. Alloploidy, nature of cross-pollination and a wide range of predominant agro-ecologies have contributed large genetic diversity of mango in India. In the present study attempt has been made with 16 traditional mango cultivars to get phenomenal insight on genetic variability and cultivars' diversity through deploying morphological and molecular markers. The cultivars were tested for consecutive two years under the aegis of All India Coordinated Research Project on Fruits, Bidhan Chandra Krishi Viswavidyalaya (BCKV), Gayeshpur, West Bengal regarding observation on 26 morphological and fruit quality parameters followed by assessing diversity at molecular level through deploying 20 SSR makers. Result showed that adequate genetic variability was existed among the tested genotypes with predominance of additive gene action regarding inheritance of most of the characters. Principal Component Analysis detected seven PCs towards contribution of more than 84.25% genetic diversity harboured by the tested genotypes. Out of 20 SSRs, 8 microsatellites were amplified and produced 27 putative alleles in 16 cultivars. Genetic Divergence through multivariate analysis, as well as through UPGMA dendrograms classified 16 mango cultivars in to five major clusters. The dendrogram revealed that Ranipasand and Gulab Khas were the most similar cultivars with 88 % similarity. Sharing of common gene pool as well as exertion of similar selection pressure during domestication as well as selection of cultivars in this region exhibited similar tradition

Studies on genetic variability and identification of sequence variations among cultivars and landraces of rice (Oryza sativa L.) for apparent amylose and amylopectin contents

Starch is the main component of cereals like rice. It is mainly composed of amylose and amylopectin. Apparent amylose content (AAC) in rice grain controlled by granule bound starch synthase enzyme (GBSS) is the key factor determining cooking and processing quality. The present study has been attempted to decipher the genetic variability among 36 cultivars of rice for agro-morphological parameters along with AAC and amylopectin content, to determine the extent of association among yield and its contributing characters and to detect the sequence variations among the cultivars having differential AAC. Results revealed the presence of adequate genetic variability among the tested rice cultivars for yield attributing traits, as well as their AAC and amylopectin content. Most of the yield attributing traits was under control of additive genes. Genetic diversity study of the tested rice cultivars grouped them into four clusters. The nucleotide sequence and derived amino acid sequence variations among the region spanning between the intron 7 and exon 10 of GBSSI allele from the rice cultivars differing in AAC detected the presence of several SNPs, deletions and insertions within the tested exon and intron regions. Presence of methionine (M) or isoleucine (I) at 308 aa position and presence of phenylalanine (F) at 425 or 434 aa position of GBSSI protein across the tested rice cultivars can be exploited in marker-assisted breeding programme for developing high yielding rice cultivars with proper grain quality as per consumer’s preference

Impact evaluation of a community engagement intervention in improving childhood immunization coverage: a cluster randomized controlled trial in Assam, India

Abstract Background To improve immunization coverage, most interventions that are part of the national immunization program in India address supply-side challenges. But, there is growing evidence that addressing demand-side factors can potentially contribute to improvement in childhood vaccination coverage in low- and middle-income countries. Participatory engagement of communities can address demand-side barriers while also mobilizing the community to advocate for better service delivery. The objective of this study is to evaluate the impact of a novel community engagement approach in improving immunization coverage. In our proposed intervention, we go a step beyond merely engaging the community and strive towards increasing ‘ownership’ by the communities. Methods/Design We adopt a cluster randomized design with two groups to evaluate the intervention in Assam, a state in the northeast region of India. To recruit villages and participants at baseline, we used a two-stage stratified random sampling method. We stratified villages; our unit of randomization, based on census data and randomly selected villages from each of the four strata. At the second-stage, we selected random sub-sample of eligible households (having children in the age group of 6–23 months) from each selected village. The study uses a repeated cross sectional design where we track the same sampled villages but draw independent random samples of households at baseline and endline. Total number of villages required for the study is 180 with 15 eligible HHs from each village. Post-baseline survey, we adopt a stratified randomization strategy to achieve better balance in intervention and control groups, leveraging information from the extensive baseline survey. Discussion The proposed intervention can help identify barriers to vaccination at the local level and potentially lead to more sustainable solutions over the long term. Our sampling design, sample size calculation, and randomization strategy address internal validity of our evaluation design. We believe that it would allow us to causally relate any observed changes in immunization coverage to the intervention. Trial registration The trial has been registered on 7th February, 2017 under the Clinical Trials Registry- India (CTRI), hosted at the ICMR’s National Institute of Medical Statistics, having registration number CTRI/2017/02/007792. This is the original study protocol

Metagenomic Insights into Rhizospheric Microbiome Profiling in Lentil Cultivars Unveils Differential Microbial Nitrogen and Phosphorus Metabolism under Rice-Fallow Ecology

The plant rhizosphere interfaces an array of microbiomes related to plant growth and development. Cultivar-specific soil microbial communities with respect to their taxonomic structure and specific function have not been investigated explicitly in improving the adaptation of lentil cultivars under rice-fallow ecology. The present study was carried out to decipher the rhizosphere microbiome assembly of two lentil cultivars under rice-fallow ecology for discerning the diversity of microbial communities and for predicting the function of microbiome genes related to nitrogen (N) and phosphorus (P) cycling processes deploying high-throughput whole (meta) genome sequencing. The metagenome profile of two cultivars detected variable microbiome composition with discrete metabolic activity. Cyanobacteria, Bacteroidetes, Proteobacteria, Gemmatimonadetes, and Thaumarchaeota were abundant phyla in the “Farmer-2” rhizosphere, whereas Actinobacteria, Acidobacteria, Firmicutes, Planctomycetes, Chloroflexi, and some incompletely described procaryotes of the “Candidatus” category were found to be robustly enriched the rhizosphere of “Moitree”. Functional prediction profiles of the microbial metagenomes between two cultivars revealed mostly house keeping genes with general metabolism. Additionally, the rhizosphere of “Moitree” had a high abundance of genes related to denitrification processes. Significant difference was observed regarding P cycling genes between the cultivars. “Moitree” with a profuse root system exhibited better N fixation and translocation ability due to a good “foraging strategy” for improving acquisition of native P under the nutrient depleted rice-fallow ecology. However, “Farmer-2” revealed a better “mining strategy” for enhancing P solubilization and further transportation to sinks. This study warrants comprehensive research for explaining the role of microbiome diversity and cultivar–microbe interactions towards stimulating microbiome-derived soil reactions regarding nutrient availability under rice-fallow ecology

Assessing the Genetic Divergence of Onion (<i>Allium Cepa</i> L.) through Morpho-Physiological and Molecular Markers

Onion is one of the most widely cultivated high-value vegetables on the globe. The study of variability and genetic divergence in this crop will facilitate judicious parent selection for future breeding. The present study incorporated 20 onion genotypes to determine the genetic variability and diversity through morpho-physiological and molecular markers. The genotypes were tested for two consecutive years to determine the effects on yield-attributing and bulb-quality traits. Diversity at a molecular level with 31 simple sequence repeat (SSR) markers was determined. Adequate genetic variability existed among the genotypes, with a predominance of additive gene action regarding the inheritance of most of the characteristics. Principal component (PC) analysis detected seven PCs contributing to more than 87% of the genetic diversity in the genotypes. Total yield (kg/plot), Grade ‘C’ bulb (equatorial diameter of the bulb between 3.5 and 4.5 cm), individual bulb weight (g) and phenol content were detected as characteristics positively associated with the first two PCs. A total of 16 primers were amplified, and they produced 32 alleles in the genotypes. Genetic diversity at the morphological and molecular levels indicated moderate diversity among the tested genotypes, with the dissolution of the genotypes into six and three clusters, respectively. The present study on onion facilitates the selection of diverse parents that can generate desirable segregants in future breeding programmes