FORMULATION AND CHARACTERIZATION OF CYCLODEXTRIN BASED CURCUMIN LOADED NANOSPONGE

Objective: The aim of proposed work is to develop and screen cyclodextrin based Nanosponge loaded with poorly soluble anticancer drug and to optimize most suitable Nanosystem with increased solubility and dissolution rate. Methods: Cyclodextrinnanosponge (CDNS) was prepared using pyromelliticdianhydride as a crosslinker for beta cyclodextrin monomer. Cyclodextrinnanosponge and curcumin were taken in 1:1 w/w proportion. The resultant curcumin loaded nanosponges were dried at 50±0.5 °C for 24 h. Results: The absorbance maxima for Curcumin was seen at 424.0 nm and for cyclodextrin was seen at 290.0 nm, The average melting point of pure drug is 181 °C which is complies with Stander melting point of drug and the aspect ratio of the nanosponge was found 1.037. Zeta potential noticed for CUR-CD-NS were more negative contrasted with separate plain CUR (−20.1±1.57) demonstrating solidness of the nanodispersion. Curcumin release from CUR-CDNS was upgraded to very nearly 10 folds toward the finish of 8 hour. Treatment with a combination of CUR-CDNS at 1:1 and 1:3 ratios resulted in an IC50 value was found 14.98 μg/ml. Conclusion: In vitro cytotoxicity study and combination index analysis showed the synergistic effect of CUR-CDNS against MCF-7 cells. The present study reveals that the combination of curcumin results in higher cytotoxicity against breast cancer cells

A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees

A revised protocol has been developed for in vitro propogation of papaya using explants from field-grown trees. Successful establishment of papaya in vitro using lateral buds could be obtained by treating the buds with Carbendazim (0.2%) and Streptomycin (0.1%) for 24h, followed by surface sterilization with mercuric chloride (0.1%) for 3 minutes and culturing on MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Established buds were proliferated on modified MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Modified MS medium supplemented with BAP (0.3 mg/l), NAA (0.1 mg/l) and GA3 (1 mg/l) caused extensive elongation of shoots. Elongated shootlets were rooted on half-strength MS medium supplemented with BAP (0.1mg/l), NAA (0.1 mg/l) and IBA (2 mg/l). Rooted plantlets were initially hardened on a potting mixture consisting of soilrite and later on a mixture of sand, soil and FYM (1:1:1)

Asymptomatic Proteinuria and Hematuria in School Going Children

Background: This study was undertaken because many cases of asymptomatic proteinuria and hematuria are present in school children.Aims and Objectives:The study was under taken to evaluate asymptomatic proteinuria and hematuria in 100 school children of both sexes from 6 to 15 years of age. Material and Methods: Samples were collected randomly from students of different classes at the Government Kannada Primary School, K H B Colony,Bijapur, Karnataka (India). The midstream urine sample was collected in a clean wide mouth jar,examined by dipsticks for proteinuria and hematuria by microscopy. Results:We found that asymptomatic proteinuria and hematuria was more prevalent amongst the children between 10 to 13 years of age group with female predominance i.e.16% proteinuria and 5% of microscopic hematuria respectively. Conclusion:By this study we conclude that it would be possible to screen a large population of children for asymptomatic proteinuria and hematuria at a relatively low cost

Integrating Fertilizer N Rates with Organics on Soil-Available Nutrients and Yield of Sapota under Semi-Arid Conditions of Karnataka

A field experiment was conducted for three consecutive years to study the effect of various combinations of nitrogenous fertilizer (in the form of urea), vermicompost and FYM on yield and soil-available nutrients in sapota. Largest number of fruits (4820 tree-1) and maximum fruit yield (31 tons ha-1) were recorded with 10kg vermicompost + 350:50:450g NPK tree-1, and was on par with application of 40kg FYM + 350:50:450g NPK tree-1. The net profit and yield trend over the years showed that application of 10kg vermicompost + 350:50:450g NPK tree-1 was more suitable for meeting nutrient requirement for enhanced yield in sapota. Application of organics (irrespective of source) showed positive, significant effect on organic matter content of the soil after three years. Highest build-up of organic matter in the soil was recorded with 10kg vermicompost alone (T10), which was at par with 40kg FYM alone (T5). Moreover, there was a clear trend of increasing total soil nitrogen content in plots supplied with increased levels of inorganic nitrogen with organic manures, and, this was subsequently reflected in potentially mineralized nitrogen, suggesting an improved labile pool of plant-available nitrogen. Therefore, there is an obvious need to include organic manures along with the inorganic nitrogenous fertilizer for optimizing the use-efficiency of soil and applied N to achieve sustainable yields in sapota for profit

Wearable Technology and Extended Applications

We are living in the turbulent flow of technology, which increases exponentially with every second we live. Technology has made our life ease, effective and enables us to do something we always dreamed of. Wearable Technology is the next step that is taken towards the next generation interaction with new modules and future designed gadgets that works with much efficiency, flexibility, accuracy and can perform various complex operation with ease. Wearable technologies are adding more layers to our life by increasing the ways we communicate and share information with each other. The continuity of these devices creates a continuous link between people that they can relate to each other. Wearable Technology is aimed to provide the real time interaction with new evolving mobile widgets and gadgets. We would see how this technology will overcome the limitations of the existing mobile gadgets and bring it to the new extended level in compare to current scenario. Wearable Technology is new technological revolution that would create a world faster reliable and secure data transmission network. We would analysis the capability, expandability and limitations of it

Stionic effects on leaf mineral nutrient contents in Pummelo (Citrus maxima Merr.) grafted on different rootstocks

A study was conducted to determine the mineral nutrients concentration in the index leaf of pummelo accessions. Index leaf samples from 25 pummelo accessions grafted on pummelo and 12 pummelo clones grafted on Rangpur lime rootstocks were collected for assessing leaf mineral nutrient status. The results revealed that pummelo plants grafted on pummelo, the concentration of leaf N (1.43-2.49 %), P (0.17-0.22 %), K (0.75-4.45 %), Ca (2.37-6.29 %), Mg (0.60-1.04 %), S (0.06-0.22 %), Fe (124-245.45 mg kg-1), Mn (9.85-50.05 mg kg-1), Zn (17-69 mg kg-1) and Cu (8.8-25.15 mg kg-1) showed significant variation with different accessions. Out of 25 pummelo accessions, twenty-four accessions were deficient in N and S, fourteen were deficient in K, four were deficient in Mn and five were deficient in Zn and all accessions were sufficient in P, Ca, Mg, Fe and Cu. The observed trends in the leaf nutrient concentration of pummelo accessions clearly indicated the significance of the genotypic variation when chemical analysis is used for diagnosing the leaf nutrient status of pummelo trees. Similarly, leaf N, P, K, Ca, Mn, Cu and Zn varied significantly among twelve pummelo clones grafted on Rangpur lime. Among the clones grafted on Rangpur lime, 18-3 and 18-5 found to have higher and lower leaf nutrient content in most of the mineral nutrients, respectively. The leaf nutrient content of pummelo varies among genotypes, but there is no genotype that stands out in all macro and micronutrients evaluated. The N, P, K, Ca, S, Fe, Mn and Cu leaf contents in pummelo were always higher in plants grafted on Rangpur lime. However, the foliar Mg and Zn contents were continually higher in plants grafted on ‘pummelo’ compared to Rangpur lime which eventually reduces leaf yellowing/chlorosis in pummelo. Pummelo rootstocks were found to respond well in terms of Mg and Zn nutrient uptake and tolerance to Phytophthora as compared to Rangpur lime. Therefore, it is concluded that pummelo can be an ideal rootstock for commercial pummelo cultivatio

Studies on Suitability of High Grade Manganese Ore of Bahadurgatta area, Chitradurga District, Karnataka in the manufacture of Electrolytic Manganese Dioxide

Manganese is one of the most widely distributed elements in the earth's crust. Manganese does not occur in nature in metallic form, but only in combination, usually in the form oxide, hydroxide, carbonate and silicate. Besides, it is important raw material in iron and steel industry, finds use in the manufacture of chem-icals and dry cells in the form of manganese dioxide. The Indian manganese dioxide ores are reported to be mostly of pyrolusite and cryptomelane and devoid of gamma or delta form

The Monoclonal Antibody Cocktail in SARS-CoV 2: A Bonanza for Patients With Chronic Lymphocytic Leukemia?

Monoclonal antibody cocktail is currently one of the most promising approaches being studied in the management of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2). We present a case of an elderly patient with coronavirus disease (COVID-19) and Chronic lymphocytic leukaemia (CLL) who had recurrent episodes of desaturation and admission in intensive care unit (ICU) despite receiving the treatment for moderate to severe COVID-19. After careful selection, weighing the benefits and risks, the patient was started on the combination of the two monoclonal antibodies, casirivimab and imdevimab. The results suggest that this could be a game changer in COVID-19 with a focused approach of management of COVID-19 positive patients especially in the vulnerable population

Bio-nanotechnology application in wastewater treatment

The nanoparticles have received high interest in the field of medicine and water purification, however, the nanomaterials produced by chemical and physical methods are considered hazardous, expensive, and leave behind harmful substances to the environment. This chapter aimed to focus on green-synthesized nanoparticles and their medical applications. Moreover, the chapter highlighted the applicability of the metallic nanoparticles (MNPs) in the inactivation of microbial cells due to their high surface and small particle size. Modifying nanomaterials produced by green-methods is safe, inexpensive, and easy. Therefore, the control and modification of nanoparticles and their properties were also discussed