Potentialités des bioélectrodes et des bioréacteurs à biomasse fixée pour l'estimation rapide de la DBO

La méthode standard de détermination de la Demande Biochimique en Oxygène (DBO-5 jours par dilutions) présente certains inconvénients bien connus dont celui de ne pas être exploitable pour la gestion en temps réel d'un procédé d'épuration. De ce fait, divers types d'appareils d'estimation rapide de la DBO, basés sur des méthodes respirométriques, ont été imaginés dans le passé et même commercialisés pour certains.Dans cette étude, des appareils d'estimation rapide de la DBO de types bioélectrode et réacteurs à biomasse fixée (écoulement piston et parfaitement mélangé) ont été mis en oeuvre, après avoir été développés ou modifiés sur base de concepts déjà existants. Une attention particulière a été portée sur la validité de ces appareils, la crédibilité de leurs mesures et la définition de leur champ d'action.De par leur principe de fonctionnement (injection en flux), la bioélectrode et le bioréacteur piston sont des appareils qui doivent être calibrés et dont la biomasse immobilisée doit être préalablement adaptée à l'échantillon à analyser. La solution de calibration est essentielle et doit être qualitativement la plus proche possible de l'échantillon à analyser.Le bioréacteur parfaitement mélangé se distingue des deux premières méthodes car il travaille théoriquement en consommation totale du substrat. La méthode ne nécessite pas de calibration mais implique, avant toute mesure de respirogramme, la détermination d'un paramètre supplémentaire, le coefficient de transfert de l'oxygène.De manière générale, la principale conclusion de l'étude réside dans la difficulté de ces appareils à fournir une DO (Demande en oxygène) corrélable à la DBO5 conventionnelle. Leur champ d'application reste en effet limité aux substrats rapidement biodégradables.Leurs potentialités restent cependant conformes aux besoins de contrôle rapide des charges organiques polluantes en entrée et sortie des stations d'épuration.BOD (Biochemical Oxygen Demand) is an important parameter to characterize organic pollution in aquatic environments and sewage. The five-day period required by the classical dilution method (BOD5) is incompatible with real-time control of a sewage treatment plant. Moreover, the assay procedure (closed respirometer, very diluted samples) is not only far from real growing conditions but also far from conditions in sewage treatment plants.Several devices for rapid BOD estimation, all based on respirometric methods, have been developed and tested. These devices can be grouped into 2 categories:- an immobilized biomass plug-flow reactor and a bioelectrode, both based on a flow injection principle; - an immobilized biomass perfectly mixed reactor, based on an open respirometer principle. We have focused mainly on validating the principles, checking the measurement reliability, and defining more precisely the scope of the various devices.The bioelectrodeThe BOD bioelectrode that we developed relies on a classical configuration that uses a yeast strain (Trichosporon cutaneum) as the biological receptor and a Clark probe as the transducer. The main changes made in this system are as follows:- a second biomembraneless Clark probe was added to the 16-ml measuring cell to serve as a reference probe in order to remove experimental disturbances (temperature, oxygen transfer coefficient, dissolved oxygen concentration, etc.). - the second change was to include the respirogram area among the data available for processing. The signal utilized in this set-up is the difference between the signals provided by the two probes. Our conclusions are as follows:- Concerning the signal processing, manufacturers are currently guided by the desire to develop devices able to estimate the BOD of a large range of substrates or effluents in a very short time (a few minutes for the newest devices). However, it seems useful, even necessary in many cases (complex mixtures of components that are oxidized at variable rates), to use information provided by the respirogram shape and area. This approach allows one to maximize the BOD bioelectrode's range for a given immobilized strain, although the trade-off is a longer total run time. - Concerning the adaptation period for microorganisms, it is impossible to correctly estimate BOD from various effluents without first adapting the biomaterial to the type of substrate to be analyzed. Therefore it is dangerous to consider a BOD bioelectrode as an analytical instrument, because an adaptation period is required after any change in the composition or, even more so, type of effluent. - Concerning the correspondence between rapid BOD and BOD5, the BOD sensor can detect only the BOD of soluble compounds that can diffuse through the biomembrane and that will be metabolized during the time of analysis. The difference observed between BOD sensor and BOD5 depends on the calibration solution but also and even more on the structure and size of the molecules constituting the sample to be analyzed. - Concerning the choice of calibration solution, choosing the right calibration solution is crucial. The calibration solution should therefore be qualitatively as close as possible to the test sample. The immobilized biomass bioreactorsThe plug-flow reactorThe plug-flow reactor design was validated for simple substrates; its working principle is similar to the bioelectrodes, since it relies on flow-injection analysis (FIA). In the case of the plug-flow reactor, the only usable information for BOD estimation is the respirogram area, as the peak height quickly reaches a rather constant value due to saturation of the immobilized microorganisms. However, the importance of the many physical and biological processes that occur concomitantly in the system (transfer, adsorption, substrate consumption, substrate saturation phenomena, dilution rate, etc.) makes a theoretical mathematical model of the reactor more difficult to establish. A long-term, more fundamental study of various natural or artificial substrates might ultimately enable us to reach such a goal.On the other hand, a variant of this reactor that recirculates the partially-degraded effluent until it is completely consumed yielded a linear correlation between system response (respirogram area) and substrate amount. In this system, oxidation of the rapidly biodegradable substrates is total under our operating conditions. This alternative reactor seems to have some very interesting possibilities, especially with regard to the automation of the system.The perfectly mixed reactorThe utilization of the perfectly mixed reactor for rapid BOD estimation is based on two sequential experiments - although this has the disadvantage of increasing the total run time - to obtain the respirogram area (S) and oxygen transfer coefficient (KL.a), as the units of the product (KL.a x S) of these parameters are equivalent to those of oxygen demand and their product is the variable that best correlates with the substrate injection volume. This correlation was observed for a large range of substrates. In the case of the perfectly mixed reactor, unlike bioelectrodes,- diffusion processes have no effect on measurement, as the substrate is consumed completely during the experiment; - the substrate consumption rate does not affect the measurements, thereby freeing the method from the influence of various experimental parameters (air flow-rate, the quantity of biomass, the liquid volume in the reactor, etc.), as our tests have shown. For a given substrate, the linearity of the correlation between the product of KL.a x S and the amount of substrate is generally excellent. The attempt to correlate the system's response with the BOD5 measurements for a variety of substrates proved to be difficult, however. Although the bioreactor's analytical range is wider than that of bioelectrodes, the system still fails to give any measurable responses for complex substrates such as starch, cellulose, proteins, etc., and the KL.a x S values estimated by the bioreactor remained much lower than the corresponding BOD5 values. KL.a x S is probably characteristic of the portion of the substrate that is degraded rapidly by the microorganisms to meet their immediate energy needs, whereas the remaining substrate is probably kept for reserve and biomass synthesis

A low-energy rate-adaptive bit-interleaved passive optical network

Energy consumption of customer premises equipment (CPE) has become a serious issue in the new generations of time-division multiplexing passive optical networks, which operate at 10 Gb/s or higher. It is becoming a major factor in global network energy consumption, and it poses problems during emergencies when CPE is battery-operated. In this paper, a low-energy passive optical network (PON) that uses a novel bit-interleaving downstream protocol is proposed. The details about the network architecture, protocol, and the key enabling implementation aspects, including dynamic traffic interleaving, rate-adaptive descrambling of decimated traffic, and the design and implementation of a downsampling clock and data recovery circuit, are described. The proposed concept is shown to reduce the energy consumption for protocol processing by a factor of 30. A detailed analysis of the energy consumption in the CPE shows that the interleaving protocol reduces the total energy consumption of the CPE significantly in comparison to the standard 10 Gb/s PON CPE. Experimental results obtained from measurements on the implemented CPE prototype confirm that the CPE consumes significantly less energy than the standard 10 Gb/s PON CPE

Neutrinos and duality

A phenomenological study of Bloom-Gilman duality is performed in electron and neutrino scattering on nuclei. In the resonance region the structure functions are calculated within the phenomenological models of Ghent and Giessen groups, where only the resonance contribution is taken into account, and the background one is neglected. Structure functions F2 in the resonance region are compared with the DIS ones, extracted directly from the experimental data. The results show, that within the models considered the Bloom-Gilman duality does not work well for nuclei: the integrated strength in the resonance region is considerably lower than in the DIS one

Influence of fractional flow reserve on grafts patency: Systematic review and patient-level meta-analysis.

To investigate the impact of invasive functional guidance for coronary artery bypass graft surgery (CABG) on graft failure. Data on the impact of fractional flow reserve (FFR) in guiding CABG are still limited. Systematic review and individual patient data meta-analysis were performed. Primary objective was the risk of graft failure, stratified by FFR. Risk estimates are reported as odds ratios (ORs) derived from the aggregated data using random-effects models. Individual patient data were analyzed using mixed effect model to assess relationship between FFR and graft failure. This meta-analysis is registered in PROSPERO (CRD42020180444). Four prospective studies comprising 503 patients referred for CABG, with 1471 coronaries, assessed by FFR were included. Graft status was available for 1039 conduits at median of 12.0 [IQR 6.6; 12.0] months. Risk of graft failure was higher in vessels with preserved FFR (OR 5.74, 95% CI 1.71-19.29). Every 0.10 FFR units decrease in the coronaries was associated with 56% risk reduction of graft failure (OR 0.44, 95% CI 0.34 to 0.59). FFR cut-off to predict graft failure was 0.79. Surgical grafting of coronaries with functionally nonsignificant stenoses was associated with higher risk of graft failure

Beyond 'Trimming the fat': the sub-editing stage of newswriting

Thus far, professional editing has not been researched extensively in writing research. This article zooms in on sub-editing in newswriting as a form of professional editing, addressing three research questions: (a) What are the ways in which a news article's text is altered?, (b) Are some types of news article altered more significantly than others?, and (c) Are certain news article sections more prone to alterations? Merging the contextualized insights of fieldwork with a corpus-based discourse analytic research perspective, we trace the differences (viz. additions, deletions, translocations, replacements) between the initial (right before sub-editing) and final (published) version of six different types of news article, (frontpage, headline, long, medium, short, and news wire article) in a corpus sample of 30 broadsheet articles. Our findings are first thatcontrary to popular belief that sub-editors mainly hack away at news stories, or merely trim the fatadditions prevail. Second, we found that most interventions occur in high-stakes articles. Third, we discovered the largest number of interventions in the entry points of an article, that is, whereaccording to eye-tracking researchreaders stop scanning and start reading. We discuss our findings in the light of training for professional newswriters

Histopathological cutaneous alterations in systemic sclerosis: a clinicopathological study

Introduction: The aims of the present study were to identify histopathological parameters which are linked to local clinical skin disease at two distinct anatomical sites in systemic sclerosis (SSc) patients with skin involvement (limited cutaneous systemic sclerosis (lcSSc) or diffuse cutaneous systemic sclerosis (dcSSc)) and to determine the sensitivity of SSc specific histological alterations, focusing on SSc patients without clinical skin involvement (limited SSc (lSSc)). Methods: Histopathological alterations were systematically scored in skin biopsies of 53 consecutive SSc patients (dorsal forearm and upper inner arm) and 18 controls (upper inner arm). Clinical skin involvement was evaluated using the modified Rodnan skin score. In patients with lcSSc or dcSSc, associations of histopathological parameters with local clinical skin involvement were determined by generalised estimation equation modelling. Results: The hyalinised collagen score, the myofibroblast score, the mean epidermal thickness, the mononuclear cellular infiltration and the frequency of focal exocytosis differed significantly between biopsies with and without local clinical skin involvement. Except for mononuclear cellular infiltration, all of the continuous parameters correlated with the local clinical skin score at the dorsal forearm. Parakeratosis, myofibroblasts and intima proliferation were present in a minority of the SSc biopsies, but not in controls. No differences were found between lSSc and controls. Conclusions: Several histopathological parameters are linked to local clinical skin disease. SSc-specific histological alterations have a low diagnostic sensitivity

Methodological framework for World Health Organization estimates of the global burden of foodborne disease

Background: The Foodborne Disease Burden Epidemiology Reference Group (FERG) was established in 2007 by the World Health Organization to estimate the global burden of foodborne diseases (FBDs). This paper describes the methodological framework developed by FERG's Computational Task Force to transform epidemiological information into FBD burden estimates. Methods and Findings: The global and regional burden of 31 FBDs was quantified, along with limited estimates for 5 other FBDs, using Disability-Adjusted Life Years in a hazard- and incidence-based approach. To accomplish this task, the following workflow was defined: outline of disease models and collection of epidemiological data; design and completion of a database template; development of an imputation model; identification of disability weights; probabilistic burden assessment; and estimating the proportion of the disease burden by each hazard that is attributable to exposure by food (i.e., source attribution). All computations were performed in R and the different functions were compiled in the R package 'FERG'. Traceability and transparency were ensured by sharing results and methods in an interactive way with all FERG members throughout the process. Conclusions: We developed a comprehensive framework for estimating the global burden of FBDs, in which methodological simplicity and transparency were key elements. All the tools developed have been made available and can be translated into a user-friendly national toolkit for studying and monitoring food safety at the local level